Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,467,813 T→C 9.7% W20R (TGG→CGG)  PP_3959 → voltage‑gated chloride channel family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,467,8130TC 9.7% 108.8 / 3.4 41W20R (TGG→CGG) PP_3959voltage‑gated chloride channel family protein
Reads supporting (aligned to +/- strand):  ref base T (19/18);  new base C (2/2);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

ACTCCCGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACCTCTTCTCATCACTCCGCTG  >  NC_002947/4467666‑4467959
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aCTCCCGGTGCGGCTACAACCTGTGTCGTGGAGGCCTGGGCGGGAAAACAAGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATg                                                                                                                                                   <  4:180028/149‑1 (MQ=255)
    ccGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCATTCTCGCCAGCGTGAACGGCgg                                                                                                                                               <  5:141626/149‑2 (MQ=255)
       gTGCGGCTACAACCTGTTTCGGGTAGGCCTGTGCTGGAAAACACGCCATACTCACGGATTTCCAAGTCAGCCATGTGTTGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAg                                                                                                                                            <  3:166839/149‑1 (MQ=255)
        tGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGc                                                                                                                                           <  8:81503/149‑1 (MQ=255)
                cAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCg                                                                                                                                   >  4:197957/1‑149 (MQ=255)
                 aaCCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGt                                                                                                                                  >  4:226777/1‑149 (MQ=255)
                      gtgtCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTg                                                                                                                              >  7:25739/1‑148 (MQ=255)
                           gggTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCgg                                                                                                                        <  3:197957/149‑1 (MQ=255)
                             gTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCgggg                                                                                                                      <  1:1550/149‑1 (MQ=255)
                                           gAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTgggg                                                                                                        >  2:70746/1‑149 (MQ=255)
                                               acacGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCg                                                                                                    >  3:220730/1‑149 (MQ=255)
                                                 acGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATAGGGGCATTGCTGGTGGGGCTCGtt                                                                                                  >  5:255582/1‑149 (MQ=255)
                                                   gCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTgc                                                                                                >  6:212518/1‑149 (MQ=255)
                                                      aTACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGTCGTCAGCGACTGGCGTTCTGGATCGTGGCATTGCTGGTGGGGCTCGTTGCGCt                                                                                             <  8:25739/149‑1 (MQ=255)
                                                          tCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCt                                                                                         >  4:125157/1‑149 (MQ=255)
                                                                cTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGcc                                                                                   <  1:70746/149‑1 (MQ=255)
                                                                     cAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCATTCTCGCCAGCGTGAACGGCgg                                                                                                                                               >  3:208262/1‑83 (MQ=37)
                                                                     cAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCATTCTCGCCAGCGTGAACGGCgg                                                                                                                                               <  4:208262/84‑2 (MQ=37)
                                                                      aaGTCAGCCATGTGTGGAGAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTGCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCt                                                                                  <  6:57226/144‑1 (MQ=255)
                                                                      aaGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCACGTTGCGCTGGCTTTCGCCTgagaa                                                                             >  5:57226/1‑145 (MQ=255)
                                                                                    tgGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGtt                                                               >  1:95140/1‑149 (MQ=255)
                                                                                      gACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCAGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCTCCTGGCTAGCCGACGCGGCGTTCg                                                             >  8:128524/1‑149 (MQ=255)
                                                                                         aaTGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCtt                                                          >  7:38924/1‑149 (MQ=255)
                                                                                         aaTGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCtt                                                          >  1:163158/1‑149 (MQ=255)
                                                                                                gACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCaa                                                   <  4:185921/149‑1 (MQ=255)
                                                                                                gACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCaa                                                   <  3:226777/149‑1 (MQ=255)
                                                                                                   ccTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCaa                                                   <  6:96129/146‑1 (MQ=255)
                                                                                                   ccTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCaa                                                   >  5:96129/1‑146 (MQ=255)
                                                                                                   ccTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAgcg                                                >  6:1017/1‑149 (MQ=255)
                                                                                                          gACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTg                                         <  2:95140/149‑1 (MQ=255)
                                                                                                              tGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAAt                                     >  6:169351/1‑149 (MQ=255)
                                                                                                                   cTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAgcg                                                <  7:219604/133‑1 (MQ=255)
                                                                                                                   cTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAgcg                                                >  8:219604/1‑133 (MQ=255)
                                                                                                                      cACTCCCCCGCCGTTCACGCTGGCTAGAATGGCGTCATCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCAc                               <  6:255582/147‑1 (MQ=255)
                                                                                                                         tCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTgg                          >  6:256434/1‑149 (MQ=255)
                                                                                                                          ctgacgccaTTCTCGCCAGCGTGAACGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGt                         >  7:221877/5‑149 (MQ=255)
                                                                                                                                   ttCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACCtcttt                >  2:241432/1‑148 (MQ=255)
                                                                                                                                   ttCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACCtcttc                <  7:128524/149‑1 (MQ=255)
                                                                                                                                     cACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACCtctttt               <  1:241432/148‑3 (MQ=255)
                                                                                                                                      aCGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACCTCTtctc              <  8:38924/148‑1 (MQ=255)
                                                                                                                                                 aaTGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACCTCTTCTCATCACTCCGCTg  <  2:163158/149‑1 (MQ=255)
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ACTCCCGGTGCGGCTACAACCTGTGTCGGGTAGGCCTGTGCGGGAAAACACGCCATACTCACGGCTTTCCAAGTCAGCCATGTGTGGACAATGTCCGACCCTACCCGACCTGCACCTTCACTCCCCCGCCGTTCACGCTGGCGAGAATGGCGTCAGCGACTGGCGTTCTGGATCGGGGCATTGCTGGTGGGGCTCGTTGCGCTGGCTTTCGCCTGGCTAGCCGACGCGGCGTTCGCTTCGTTCAAGCGCTTGCTGGAATGGGCACCCTGGTCACCTCTTCTCATCACTCCGCTG  >  NC_002947/4467666‑4467959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑