Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,474,259 A→G 7.2% L426P (CTG→CCG)  PP_3964 ← transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,474,2590AG 7.2% 165.0 / 2.3 55L426P (CTG→CCG) PP_3964transposase
Reads supporting (aligned to +/- strand):  ref base A (22/29);  new base G (2/2);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

GCCGACTGGATCAAACTCATGATTGCCGCAGCCCGCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTCATGCAGTTTTTTGAGGATCGGCACCGCCAATTCTTGTC  >  NC_002947/4474115‑4474397
                                                                                                                                                |                                                                                                                                          
gcCGACTGGATCAAACTCATGATTGCCGCAGCCCGCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAgg                                                                                                                                          >  6:212193/1‑147 (MQ=255)
gcCGACTGGATCAAACTCATGATTGCCGCAGCCCGCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTa                                                                                                                                        <  8:33839/149‑1 (MQ=255)
        gATCAAACTCATGATTGCCGCAGCCCGCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGt                                                                                                                                  >  3:69263/1‑147 (MQ=255)
            aaaCTCATGATTGCCGCAGCCCGCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAgcgc                                                                                                                            <  4:250464/149‑1 (MQ=255)
                tCATGATTGCCGCAGCCCGCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCTGACGCGCTAcc                                                                                                                        >  3:71631/1‑149 (MQ=255)
                tCATGATTGCCGCAGCCCGCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTAcc                                                                                                                        >  8:34816/1‑149 (MQ=255)
                     aTTGCCGCAGCCCGCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCTGACGCGCTAcctgga                                                                                                                    <  4:71631/148‑5 (MQ=18)
                     aTTGCCGCAGCCCGCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCg                                                                                                                   >  2:163818/1‑149 (MQ=255)
                     aTTGCCGCAGCCCGCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCg                                                                                                                   <  5:33590/149‑1 (MQ=255)
                          cGCAGCCCGCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCa                                                                                                              <  5:212193/149‑1 (MQ=255)
                           gCAGCCCGCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGTCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAg                                                                                                             <  7:192412/149‑1 (MQ=255)
                           gCAGCCCGCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAgg                                                                                                                                          >  6:269244/1‑120 (MQ=255)
                           gCAGCCCGCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAgg                                                                                                                                          <  5:269244/120‑1 (MQ=255)
                                ccGCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCGGGTAGCGCTGACGCGCTAcctgga                                                                                                                    >  7:116761/1‑133 (MQ=18)
                                ccGCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCGGGTAGCGCTGACGCGCTAcctgga                                                                                                                    <  8:116761/137‑5 (MQ=18)
                                  gCTTACCGCTGCGCGGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTaa                                                                                                      <  3:146850/149‑1 (MQ=255)
                                  gCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAgg                                                                                                                                          >  7:19859/1‑113 (MQ=255)
                                  gCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAgg                                                                                                                                          <  8:19859/113‑1 (MQ=255)
                                      aCCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGAGCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATc                                                                                                    <  2:171489/147‑1 (MQ=255)
                                       ccGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGa                                                                                                  <  1:163818/148‑1 (MQ=255)
                                       ccGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAg                                                                                                 <  6:165154/149‑1 (MQ=255)
                                           tGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTAGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCt                                                                                             >  6:268906/1‑149 (MQ=255)
                                                   gATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGt                                                                                     <  1:34876/149‑1 (MQ=255)
                                                       ccGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCGGGTAGCGCTGACGCGCTAcctgga                                                                                                                    <  7:228526/114‑5 (MQ=14)
                                                       ccGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCGGGTAGCGCTGACGCGCTAcctgga                                                                                                                    >  8:228526/1‑110 (MQ=14)
                                                         gCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGa                                                                               >  5:196930/1‑149 (MQ=255)
                                                         gCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGa                                                                               <  8:158664/149‑1 (MQ=255)
                                                          acaacaaccaggtcgaaaaccagatacggccatgggcactcgggcgctcgaactggttGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTg                                                                                    <  4:14783‑M2/85‑1 (MQ=255)
                                                          acaacaaccaggtcgaaaaccagatacggccatgggcactcgggcgctcgaactggttGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTg                                                                                    >  3:14783‑M2/59‑143 (MQ=255)
                                                           aaaTAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTCGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATc                                                                             <  5:268906/149‑1 (MQ=255)
                                                             aTAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCa                                                                           >  2:100014/1‑149 (MQ=255)
                                                              caaccaggtcgaaaaccagatacggccatgggcactcgggcgctcgaactggttGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCAt                                                                          >  4:17623‑M2/55‑149 (MQ=255)
                                                                ACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCAtt                                                                         >  4:69263‑M1/1‑148 (MQ=255)
                                                                 CCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGt                                                                                                                                  <  3:236404‑M1/90‑1 (MQ=255)
                                                                 CCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGt                                                                                                                                  >  4:236404‑M1/1‑90 (MQ=255)
                                                                                   tGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAg                                                     <  7:34816/149‑1 (MQ=255)
                                                                                      ccATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGcc                                                   >  7:71768/1‑148 (MQ=255)
                                                                                        aTGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAAc                                                <  1:100014/149‑1 (MQ=255)
                                                                                             cGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATc                                             >  3:20760/1‑147 (MQ=255)
                                                                                                     gTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTCATg                                     <  4:20760/147‑1 (MQ=255)
                                                                                                            aCCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTCATGCAGtttttt                            <  8:71768/149‑1 (MQ=255)
                                                                                                              ctggttGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTCATGCAGTTTTTTGa                          <  6:196930‑M2/143‑1 (MQ=255)
                                                                                                              ctggttGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTCATGCAGTTTTTTGa                          <  1:249590‑M2/143‑1 (MQ=255)
                                                                                                                   tGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTCATGCAGTTTTTTGAGGATc                     >  5:6270‑M2/2‑149 (MQ=255)
                                                                                                                     ttgtCGATGGGCACAGGCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTCATGCAGTTTTTTGAGGATCg                    <  7:229544/148‑1 (MQ=255)
                                                                                                                        tCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTCATGCAGtttttt                            >  6:73290/1‑137 (MQ=255)
                                                                                                                        tCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTCATGCAGtttttt                            <  5:73290/137‑1 (MQ=255)
                                                                                                                          gATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTCATGCAGTTTTTTGAGGATCGGCAcc               <  8:82684/148‑1 (MQ=255)
                                                                                                                          gATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTCATGCAGTTTTTTGAGGATCGGCACCg              >  3:209745/1‑149 (MQ=255)
                                                                                                                            tGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTCATGCAGTTTTTTGAGGATCGGCACCGc             <  4:209745/148‑1 (MQ=255)
                                                                                                                                 acaGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTCATGCAGTTTTTTGAGGATCGGCACCGCCAATTc       >  7:161987/1‑149 (MQ=255)
                                                                                                                                 acaGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTCATGCAGTTTTTTGAGGATCGGCACCGCCAATTc       >  3:49560/1‑149 (MQ=255)
                                                                                                                                      cccATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTCATGCAGTTTTTTGAGGATCGGCACCGCCAATTCTTGTc  <  4:49560/149‑1 (MQ=255)
                                                                                                                                               cAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTc                                        <  8:225345/102‑1 (MQ=255)
                                                                                                                                               cAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTc                                        >  7:225345/1‑102 (MQ=255)
                                                                                                                                                |                                                                                                                                          
GCCGACTGGATCAAACTCATGATTGCCGCAGCCCGCTTACCGCTGCGCAGGGATCCCGCAAATAACCAGTTCGAGCGCCCGAGTGCCCATGGCCGTATCTGGTTTTCGACCTGGTTGTTGTCGATGGGCACAGCCCCATCATCCAGGTAGCGCGTCAGCGCTACCCAGCGTTTCAGGCTGTAATCGAGGGCTTTGGCCGTGGCTGATCCATTGGGCACCAGGTCTCGCTGAGCCAACATCCAGTCATGCAGTTTTTTGAGGATCGGCACCGCCAATTCTTGTC  >  NC_002947/4474115‑4474397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑