Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,477,817 G→C 6.9% H59D (CAT→GAT)  PP_3968 ← sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,477,8170GC 6.9% 176.1 / 4.0 58H59D (CAT→GAT) PP_3968sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (25/29);  new base C (2/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

TCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAA  >  NC_002947/4477679‑4477951
                                                                                                                                          |                                                                                                                                      
tCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCt                                                                                                                                >  6:4157/1‑147 (MQ=255)
tCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTACGAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCt                                                                                                                                >  2:255331/1‑147 (MQ=255)
  gAGCTCAGCGCTCGGTACTCAGAGACCTATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGc                                                                                                                            <  5:137018/149‑1 (MQ=255)
  gAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTgcg                                                                                                                             >  8:102848/1‑146 (MQ=255)
  gAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAg                                                                                                                             <  3:52142/148‑1 (MQ=255)
  gAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCGCATCAGCAGTCTGa                                                                                                                              <  7:1414/147‑1 (MQ=255)
   agcgcagcgcTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCa                                                                                                                           <  7:100379/149‑1 (MQ=255)
    ccgcagcgcTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAg                                                                                                                          <  5:202951/148‑1 (MQ=255)
      gcagcgcTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTACGAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTa                                                                                                                        <  1:255331/149‑1 (MQ=255)
       cagcgcTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTAc                                                                                                                       <  2:90486/149‑1 (MQ=255)
       cagcgcTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTAc                                                                                                                       <  3:100170/149‑1 (MQ=255)
                   cTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCg                                                                                                            >  6:236483/1‑148 (MQ=255)
                   cTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCg                                                                                                            <  3:228168/148‑1 (MQ=255)
                   cTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGGCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCg                                                                                                            <  8:195604/148‑1 (MQ=255)
                     cAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGc                                                                                                          >  6:240791/1‑148 (MQ=255)
                           ccAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTg                                                                                                   <  4:96255/149‑1 (MQ=255)
                              aTTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGGCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTgcg                                                                                                 >  1:87345/1‑148 (MQ=255)
                                   tCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTTCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAAt                                                                                           <  1:262017/149‑1 (MQ=255)
                                   tCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATAAGCGGCAACGGGGCGCAGAAt                                                                                           >  7:104380/1‑149 (MQ=255)
                                      aTCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAAtctt                                                                                        <  5:94631/149‑1 (MQ=255)
                                       tCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAAtcttc                                                                                       <  2:198910/149‑1 (MQ=255)
                                         gAAAAGCCAGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTtctc                                                                                     <  5:264341/149‑1 (MQ=255)
                                                 cGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAg                                                                                                                             <  3:198134/101‑1 (MQ=255)
                                                 cGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAg                                                                                                                             >  4:198134/1‑101 (MQ=255)
                                                     aTCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCt                                                                         >  3:151500/1‑149 (MQ=255)
                                                      tCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCt                                                                         <  5:4157/148‑1 (MQ=255)
                                                             aGTAATGGCTTGGGGCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAAGCTTCTCAGAGTGATTCCTGAGGCCgg                                                                 <  4:139231/149‑1 (MQ=255)
                                                             aGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCa                                                                                    <  8:103841/130‑1 (MQ=255)
                                                             aGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCa                                                                                    >  7:103841/1‑130 (MQ=255)
                                                             aGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCgg                                                                 >  2:42381/1‑149 (MQ=255)
                                                                  tGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCt                                                               >  4:80984/1‑146 (MQ=255)
                                                                  tGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCt                                                               <  3:80984/146‑1 (MQ=255)
                                                                          gTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGt                                                    <  5:240791/149‑1 (MQ=255)
                                                                                gccgccGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTaa                                              >  2:16278/1‑149 (MQ=255)
                                                                                         tCGCTGCTCATTAGAGGGGCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCtt                                     <  2:87345/149‑1 (MQ=255)
                                                                                           gctgctCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGa                                   <  5:236483/149‑1 (MQ=255)
                                                                                           gctgctCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGACTGAATCCTGAGTGGGTAAGTCCGTCTTGa                                   >  7:195124/1‑149 (MQ=255)
                                                                                              gctCATTAGAGTGCCTCTGCCTTGCGGCTTCTCAGACTTCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGTGTAagtcagtc                                       <  4:179185/142‑1 (MQ=255)
                                                                                              gctCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTc                                       >  3:179185/1‑142 (MQ=255)
                                                                                              gctCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGAcc                                 >  3:58035/1‑148 (MQ=255)
                                                                                                  aTTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACg                            >  1:130419/1‑149 (MQ=255)
                                                                                                     agagGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGc                         >  6:104025/1‑149 (MQ=255)
                                                                                                         ggCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGaaa                     >  3:224430/1‑149 (MQ=255)
                                                                                                            ctctGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAAtcc                  >  4:178935/1‑149 (MQ=255)
                                                                                                                 ccTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAAtcctc                <  5:104025/146‑1 (MQ=255)
                                                                                                                 ccTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAAtcctc                <  4:151500/146‑1 (MQ=255)
                                                                                                                   ttGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAg           >  1:35574/1‑149 (MQ=255)
                                                                                                                       gcCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTg       <  4:224430/149‑1 (MQ=255)
                                                                                                                         cTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGt                           <  7:21212/127‑1 (MQ=255)
                                                                                                                         cTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGt                           >  8:21212/1‑127 (MQ=255)
                                                                                                                           tctcAGACTGCTGATGCGCACTCTGAGGAGTACGCATATCAGCGGCAAAGCTGGGCAGAATCTTCTAAGAGTGATTCCTGAGGCCGGGTGAATCCTGAGTGGGAAAGTCCTTCTTGACCGAACGTGCGAAATCCTCACAAGCCTGgag    >  6:76124/1‑148 (MQ=255)
                                                                                                                           actcAGACTGCGCATCAGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAAtc                   >  4:48197/2‑133 (MQ=255)
                                                                                                                           actcAGACTGCGCATCAGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAAtc                   <  3:48197/132‑1 (MQ=255)
                                                                                                                            ctcAGACTGCGCATCAGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCCGCTGAATCCTGAGTGGGGAAGTCCGTCTTGACCGACCGTGCGAAATCCTCCCAAGCCTGGAGaa  >  8:197846/1‑149 (MQ=255)
                                                                                                                               aGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAAtcctc                <  4:146269/132‑1 (MQ=255)
                                                                                                                               aGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAAtcctc                >  3:146269/1‑132 (MQ=255)
                                                                                                                                   tgctgATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGaa                      >  2:167253/1‑122 (MQ=255)
                                                                                                                                   tgctgATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGaa                      <  1:167253/122‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                      
TCGAGCGCAGCGCTCGGTACTCAGAGACCAATTGATCCATCGAAAAGCCCGCTATCAAACGAGTAATGGCATGGGTCTGAGCCGCCGTATCGCTGCTCATTAGAGGGCCTCTGCCTTGCGCCTTCTCAGACTGCTGATGCGCAGTCTGAGCAGTACGCATATCAGCGGCAACGGTGCGCAGAATCTTCTCAGAGTGATTCCTGAGGCCGGCTGAATCCTGAGTGGGTAAGTCCGTCTTGACCGAACGTGCGAAATCCTCCCAAGCCTGGAGAA  >  NC_002947/4477679‑4477951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑