BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,522,239	T→G	13.5%	F30V (TTC→GTC) ‡	idh →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,522,239	0	T	G	13.5%	87.8 / 7.6	37	F30V (TTC→GTC) ‡	idh	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (15/17); new base G (2/3); total (17/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

CCTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCTGAAG  >  NC_002947/4522101‑4522386

ccTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTc                                                                                                                                           <  5:65669/149‑1 (MQ=255)

ccTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGAAGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTc                                                                                                                                           >  2:102219/1‑149 (MQ=255)

    cgcACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGCATCATCGAAGCGGTGAACGCTTCgat                                                                                                                                        <  1:140047/149‑3 (MQ=255)

    cgcACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCgg                                                                                                                                         <  5:37274/147‑1 (MQ=255)

     gcACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTg                                                                                                                                      <  1:175240/149‑1 (MQ=255)

           cGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCg                                                                                                                                <  1:145416/149‑1 (MQ=255)

              gaCTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGGTGAACGCTTCga                                                                                                                                         >  5:116695/1‑136 (MQ=255)

              gaCTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGGTGAACGCTTCga                                                                                                                                         <  6:116695/137‑2 (MQ=255)

               aCTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGGTGAACGGTTCga                                                                                                                                         <  3:174658/136‑2 (MQ=255)

               aCTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGGTGAACGCTTCga                                                                                                                                         >  4:174658/1‑135 (MQ=255)

                      cACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCAGTCGAAAca                                                                                                                     >  8:140269/1‑148 (MQ=255)

                                    agagagTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCct                                                                                                       >  4:225496/1‑149 (MQ=255)

                                    agagagTTAACCCGCATGCACACCCGTTACAAGATCATATATACCATCACCGACAAAGCCCCAGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAACCGTTCACCGCTTAGGCTGACATCGCTGTCGAAACTCGCGACATTTACct                                                                                                       >  5:81180/1‑149 (MQ=255)

                                            aaCCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGcc                                                                                               >  1:42895/1‑149 (MQ=255)

                                             aCCCGCATGCCCAGCCGTTCCAATATCATCTATACCTTGACTGACAAAGCCCCCGCCCTCGCCACTTACTCTCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGTCTGGcc                                                                                               <  6:81180/148‑1 (MQ=255)

                                              cccGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCg                                                                                              >  3:26029/1‑148 (MQ=255)

                                               ccGCATGCCAACCCTTTCCAATATAATATATAACTTCACCTACGAAGCCACCGCCCTCGCCACTTACTCGCTTCTGACG‑ATCATCTAAGCGTTCAACGCTTCGGCTCACATCCCTGTCGAAACTCGCGACAATTCCCTGGCTGCCCCTa                                                                                            >  8:198451/1‑149 (MQ=255)

                                                       ccACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTgcc                                                                                    <  4:221955/149‑1 (MQ=255)

                                                         aCCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACATACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTgccgc                                                                                  >  4:3144/1‑149 (MQ=255)

                                                             gTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTc                                                                              >  1:114933/1‑149 (MQ=255)

                                                                    gATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGc                                                                       <  7:198451/149‑1 (MQ=255)

                                                                      tcatcTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACATACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCaa                                                                     <  3:3144/149‑1 (MQ=255)

                                                                         tcTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTg                                                                  >  6:156217/1‑149 (MQ=255)

                                                                            ataCCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGa                                                                         <  4:34177/139‑1 (MQ=255)

                                                                            ataCCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGa                                                                         >  3:34177/1‑139 (MQ=255)

                                                                                cTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCT‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGgcg                                                              >  4:133892/1‑146 (MQ=255)

                                                                                cTTCACCGACGAAGCCCCCGCCATCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGgcg                                                              >  5:209420/1‑146 (MQ=255)

                                                                                    aCCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGaa                                                       <  1:102219/149‑1 (MQ=255)

                                                                                    aCCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGaa                                                       <  5:232735/149‑1 (MQ=255)

                                                                                    aCCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGaa                                                       <  4:26029/149‑1 (MQ=255)

                                                                                               ccccGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCTTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGc                                             >  8:238531/1‑148 (MQ=255)

                                                                                                       tCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTg                                    >  5:187219/1‑149 (MQ=255)

                                                                                                         gCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTgg                                   <  5:156217/148‑1 (MQ=255)

                                                                                                          ccACTTACTCGCTGCTGCCG‑TTCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCg                                 <  7:140269/149‑1 (MQ=255)

                                                                                                                           ccG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGc                <  6:187219/149‑1 (MQ=255)

                                                                                                                                  atcGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTaccac          <  2:42895/149‑1 (MQ=255)

                                                                                                                                          gTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCTGAAg  <  3:225496/149‑1 (MQ=255)

CCTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCTGAAG  >  NC_002947/4522101‑4522386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`