Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,522,243	C→A	13.7%	T31N (ACC→AAC)	idh →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,522,243	0	C	A	13.7%	105.6 / 6.9	42	T31N (ACC→AAC)	idh	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (17/19);  new base A (3/3);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

CCTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCTGAAGCCAA  >  NC_002947/4522101‑4522390                                                                                                                                                |                                                                                                                                                    ccTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTc                                                                                                                                               <  5:65669/149‑1 (MQ=255) ccTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGAAGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTc                                                                                                                                               >  2:102219/1‑149 (MQ=255)     cgcACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGCATCATCGAAGCGGTGAACGCTTCgat                                                                                                                                            <  1:140047/149‑3 (MQ=255)     cgcACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCgg                                                                                                                                             <  5:37274/147‑1 (MQ=255)      gcACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTg                                                                                                                                          <  1:175240/149‑1 (MQ=255)            cGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCg                                                                                                                                    <  1:145416/149‑1 (MQ=255)               gaCTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGGTGAACGCTTCga                                                                                                                                             >  5:116695/1‑136 (MQ=255)               gaCTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGGTGAACGCTTCga                                                                                                                                             <  6:116695/137‑2 (MQ=255)                aCTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGGTGAACGGTTCga                                                                                                                                             <  3:174658/136‑2 (MQ=255)                aCTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGGTGAACGCTTCga                                                                                                                                             >  4:174658/1‑135 (MQ=255)                       cACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCAGTCGAAAca                                                                                                                         >  8:140269/1‑148 (MQ=255)                                     agagagTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCct                                                                                                           >  4:225496/1‑149 (MQ=255)                                     agagagTTAACCCGCATGCACACCCGTTACAAGATCATATATACCATCACCGACAAAGCCCCAGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAACCGTTCACCGCTTAGGCTGACATCGCTGTCGAAACTCGCGACATTTACct                                                                                                           >  5:81180/1‑149 (MQ=255)                                             aaCCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGcc                                                                                                   >  1:42895/1‑149 (MQ=255)                                              aCCCGCATGCCCAGCCGTTCCAATATCATCTATACCTTGACTGACAAAGCCCCCGCCCTCGCCACTTACTCTCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGTCTGGcc                                                                                                   <  6:81180/148‑1 (MQ=255)                                               cccGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCg                                                                                                  >  3:26029/1‑148 (MQ=255)                                                ccGCATGCCAACCCTTTCCAATATAATATATAACTTCACCTACGAAGCCACCGCCCTCGCCACTTACTCGCTTCTGACG‑ATCATCTAAGCGTTCAACGCTTCGGCTCACATCCCTGTCGAAACTCGCGACAATTCCCTGGCTGCCCCTa                                                                                                >  8:198451/1‑149 (MQ=255)                                                        ccACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTgcc                                                                                        <  4:221955/149‑1 (MQ=255)                                                          aCCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACATACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTgccgc                                                                                      >  4:3144/1‑149 (MQ=255)                                                              gTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTc                                                                                  >  1:114933/1‑149 (MQ=255)                                                                     gATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGc                                                                           <  7:198451/149‑1 (MQ=255)                                                                       tcatcTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACATACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCaa                                                                         <  3:3144/149‑1 (MQ=255)                                                                          tcTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTg                                                                      >  6:156217/1‑149 (MQ=255)                                                                             ataCCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGa                                                                             <  4:34177/139‑1 (MQ=255)                                                                             ataCCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGa                                                                             >  3:34177/1‑139 (MQ=255)                                                                                 cTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCT‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGgcg                                                                  >  4:133892/1‑146 (MQ=255)                                                                                 cTTCACCGACGAAGCCCCCGCCATCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGgcg                                                                  >  5:209420/1‑146 (MQ=255)                                                                                     aCCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGaa                                                           <  1:102219/149‑1 (MQ=255)                                                                                     aCCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGaa                                                           <  4:26029/149‑1 (MQ=255)                                                                                     aCCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGaa                                                           <  5:232735/149‑1 (MQ=255)                                                                                                ccccGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCTTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGc                                                 >  8:238531/1‑148 (MQ=255)                                                                                                        tCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTg                                        >  5:187219/1‑149 (MQ=255)                                                                                                          gCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTgg                                       <  5:156217/148‑1 (MQ=255)                                                                                                           ccACTTACTCGCTGCTGCCG‑TTCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCg                                     <  7:140269/149‑1 (MQ=255)                                                                                                                            ccG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGc                    <  6:187219/149‑1 (MQ=255)                                                                                                                                   atcGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTaccac              <  2:42895/149‑1 (MQ=255)                                                                                                                                           gTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCTGAAg      <  3:225496/149‑1 (MQ=255)                                                                                                                                             tCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAg                                                      >  4:229642/1‑99 (MQ=255)                                                                                                                                             tCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAg                                                      <  3:229642/99‑1 (MQ=255)                                                                                                                                               aCCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGc                                      <  7:27321/113‑1 (MQ=255)                                                                                                                                               aCCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGc                                      >  8:27321/1‑113 (MQ=255)                                                                                                                                               aCCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCTGAAGCCaa  >  7:178807/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                    CCTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCG‑ATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCTGAAGCCAA  >  NC_002947/4522101‑4522390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑