Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,549,179 A→G 9.8% intergenic (+51/‑85) pydB → / → pydX bifunctional D‑hydantoinase/dihydropyrimidinase/NADP‑dependent dihydropyrimidine dehydrogenase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,549,1790AG 9.8% 140.2 / 5.5 51intergenic (+51/‑85)pydB/pydXbifunctional D‑hydantoinase/dihydropyrimidinase/NADP‑dependent dihydropyrimidine dehydrogenase subunit
Reads supporting (aligned to +/- strand):  ref base A (23/23);  new base G (2/3);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

GCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGCCGAGC  >  NC_002947/4549031‑4549324
                                                                                                                                                    |                                                                                                                                                   
gcgcGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACaa                                                                                                                                                     <  2:194693/149‑1 (MQ=255)
    ggggCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAg                                                                                                                                                    >  8:34304/1‑146 (MQ=255)
    ggggCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAg                                                                                                                                                    <  7:34304/146‑1 (MQ=255)
    ggggCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GAc                                                                                                                                                >  3:57697/1‑149 (MQ=255)
    ggggCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCAGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GAc                                                                                                                                                >  6:249271/1‑149 (MQ=255)
                  aaCGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCtgtg                                                                                                                                  <  3:211870/149‑1 (MQ=255)
                        cggcGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAGGCGATCGCGGTC‑CTTGTGGGAGCg                                                                                                                            <  6:194059/149‑1 (MQ=38)
                            gTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGccc                                                                                                                        <  3:186470/149‑1 (MQ=255)
                                ccGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAg                                                                                                                     >  7:194500/1‑148 (MQ=255)
                                       tgtATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGAcc                                                                                                             <  4:57697/149‑1 (MQ=255)
                                           tGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCaa                                                                                                         >  8:101980/1‑149 (MQ=255)
                                                  cTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGt                                                                                                  >  8:226274/1‑149 (MQ=255)
                                                     gggCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGc                                                                                               <  2:31264/149‑1 (MQ=255)
                                                      ggCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGcc                                                                                              >  8:148970/1‑149 (MQ=255)
                                                         gcCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGc                                                                                           <  7:80924/149‑1 (MQ=255)
                                                           cGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCAc                                                                                         >  2:197977/1‑149 (MQ=255)
                                                              gCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCa                                                                                       >  2:135944/1‑148 (MQ=255)
                                                               ccGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAGGCGATCGCGGTC‑CTTGTGGGAGCGGc                                                                                                                          >  8:205949/1‑112 (MQ=25)
                                                               ccGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAGGCGATCGCGGTC‑CTTGTGGGAGCGGc                                                                                                                          <  7:205949/112‑1 (MQ=25)
                                                                         gCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATAACTGTCGCCTGCACCAAAAAAAATa                                                                              >  6:164671/1‑146 (MQ=255)
                                                                            gCCGATCCCGGTTCCGCGCTGACGTCATTGGGGCTGCGGTGCAGCCCAGCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAgg                                                                        <  4:169720/149‑1 (MQ=39)
                                                                               gATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTa                                                                     <  7:101980/149‑1 (MQ=255)
                                                                                 tGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATa                                                                              <  2:204009/138‑1 (MQ=255)
                                                                                 tGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATa                                                                              >  1:204009/1‑138 (MQ=255)
                                                                                    cTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATagaga                                                                          >  5:245123/1‑139 (MQ=255)
                                                                                    cTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATagaga                                                                          <  6:245123/139‑1 (MQ=255)
                                                                                          cgcgCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGAc                                                          <  5:164671/148‑1 (MQ=255)
                                                                                          cgcgCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGATAC‑AACCGTGAc                                                          >  6:213843/1‑148 (MQ=255)
                                                                                             gcTGAAGTCATTGGGGCTGCTGTGCAGCCAATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTTTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAAc                                                      <  1:124196/149‑1 (MQ=255)
                                                                                               tGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCAGTCGCCTGCACCAAAAAAAATATAGAGGCTAC‑AAACGTGACCAACGc                                                    >  8:56695/1‑149 (MQ=255)
                                                                                                gAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATag                                                                             <  8:60622/124‑1 (MQ=255)
                                                                                                 aaGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGccc                                                  >  6:203332/1‑149 (MQ=255)
                                                                                                  aGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCt                                                 <  5:249271/149‑1 (MQ=255)
                                                                                                     cATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCCCCAAAAAAAATAGAGAGGCTAC‑AACCGTGCCCAACGCCCTGac                                              >  3:22860/1‑148 (MQ=255)
                                                                                                      aTTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATa                                                                              <  7:97981/117‑1 (MQ=255)
                                                                                                      aTTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATa                                                                              >  8:97981/1‑117 (MQ=255)
                                                                                                      aTTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGa                                               <  7:148970/147‑1 (MQ=255)
                                                                                                      aTTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAAc                                             >  2:192198/1‑149 (MQ=255)
                                                                                                          gtgctgctGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCAt                                          <  7:56695/146‑1 (MQ=255)
                                                                                                           ggCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCAttt                                        <  1:197977/149‑1 (MQ=255)
                                                                                                                    gCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACC‑AAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGTCGCGcccc                              >  4:251810/1‑149 (MQ=255)
                                                                                                                        cccATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACC‑AAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGTCGCGCCCCCggg                          <  3:251810/149‑1 (MQ=255)
                                                                                                                           aTCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGcc                        >  6:171349/1‑149 (MQ=255)
                                                                                                                            tCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTTGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGATCCATTTGCCGCGCCCCCGGGCCg                       <  7:161928/149‑1 (MQ=255)
                                                                                                                              gcgACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCgg                      <  7:226274/148‑1 (MQ=255)
                                                                                                                               cgACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTACAAACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGgc                     <  1:192198/149‑1 (MQ=255)
                                                                                                                                     aaGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGAc               >  3:97561/1‑148 (MQ=255)
                                                                                                                                        gCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGt           <  1:135944/149‑1 (MQ=255)
                                                                                                                                           gCTCCCACAGGCGATCGCGGTC‑CTTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGAc               <  7:43984/142‑1 (MQ=38)
                                                                                                                                           gCTCCCACAGGCGATCGCGGTC‑CTTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGAc               >  8:43984/1‑142 (MQ=38)
                                                                                                                                              cccACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGcc      >  4:227274/1‑148 (MQ=255)
                                                                                                                                                 acaAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGCCCAGTTGGCCGAgc  >  4:5855/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGCCGAGC  >  NC_002947/4549031‑4549324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑