| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,549,183 | C→T | 10.2% | intergenic (+55/‑81) | pydB → / → pydX | bifunctional D‑hydantoinase/dihydropyrimidinase/NADP‑dependent dihydropyrimidine dehydrogenase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,549,183 | 0 | C | T | 10.2% | 132.4 / 5.1 | 49 | intergenic (+55/‑81) | pydB/pydX | bifunctional D‑hydantoinase/dihydropyrimidinase/NADP‑dependent dihydropyrimidine dehydrogenase subunit |
| Reads supporting (aligned to +/- strand): ref base C (22/22); new base T (2/3); total (24/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
GGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGCCGAGCGCTT > NC_002947/4549035‑4549328 | ggggCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GAc > 3:57697/1‑149 (MQ=255)ggggCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCAGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GAc > 6:249271/1‑149 (MQ=255) aaCGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCtgtg < 3:211870/149‑1 (MQ=255) cggcGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAGGCGATCGCGGTC‑CTTGTGGGAGCg < 6:194059/149‑1 (MQ=38) gTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGccc < 3:186470/149‑1 (MQ=255) ccGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAg > 7:194500/1‑148 (MQ=255) tgtATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGAcc < 4:57697/149‑1 (MQ=255) tGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCaa > 8:101980/1‑149 (MQ=255) cTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGt > 8:226274/1‑149 (MQ=255) gggCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGc < 2:31264/149‑1 (MQ=255) ggCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGcc > 8:148970/1‑149 (MQ=255) gcCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGc < 7:80924/149‑1 (MQ=255) cGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCAc > 2:197977/1‑149 (MQ=255) gCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCa > 2:135944/1‑148 (MQ=255) ccGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAGGCGATCGCGGTC‑CTTGTGGGAGCGGc < 7:205949/112‑1 (MQ=25) ccGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAGGCGATCGCGGTC‑CTTGTGGGAGCGGc > 8:205949/1‑112 (MQ=25) gCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATAACTGTCGCCTGCACCAAAAAAAATa > 6:164671/1‑146 (MQ=255) gCCGATCCCGGTTCCGCGCTGACGTCATTGGGGCTGCGGTGCAGCCCAGCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAgg < 4:169720/149‑1 (MQ=39) gATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTa < 7:101980/149‑1 (MQ=255) tGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATa < 2:204009/138‑1 (MQ=255) tGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATa > 1:204009/1‑138 (MQ=255) cTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATagaga > 5:245123/1‑139 (MQ=255) cTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATagaga < 6:245123/139‑1 (MQ=255) cgcgCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGAc < 5:164671/148‑1 (MQ=255) cgcgCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGATAC‑AACCGTGAc > 6:213843/1‑148 (MQ=255) gcTGAAGTCATTGGGGCTGCTGTGCAGCCAATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTTTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAAc < 1:124196/149‑1 (MQ=255) tGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCAGTCGCCTGCACCAAAAAAAATATAGAGGCTAC‑AAACGTGACCAACGc > 8:56695/1‑149 (MQ=255) gAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATag < 8:60622/124‑1 (MQ=255) aaGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGccc > 6:203332/1‑149 (MQ=255) aGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCt < 5:249271/149‑1 (MQ=255) cATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCCCCAAAAAAAATAGAGAGGCTAC‑AACCGTGCCCAACGCCCTGac > 3:22860/1‑148 (MQ=255) aTTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATa < 7:97981/117‑1 (MQ=255) aTTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATa > 8:97981/1‑117 (MQ=255) aTTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGa < 7:148970/147‑1 (MQ=255) aTTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAAc > 2:192198/1‑149 (MQ=255) gtgctgctGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCAt < 7:56695/146‑1 (MQ=255) ggCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCAttt < 1:197977/149‑1 (MQ=255) gCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACC‑AAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGTCGCGcccc > 4:251810/1‑149 (MQ=255) cccATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACC‑AAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGTCGCGCCCCCggg < 3:251810/149‑1 (MQ=255) aTCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGcc > 6:171349/1‑149 (MQ=255) tCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTTGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGATCCATTTGCCGCGCCCCCGGGCCg < 7:161928/149‑1 (MQ=255) gcgACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCgg < 7:226274/148‑1 (MQ=255) cgACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTACAAACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGgc < 1:192198/149‑1 (MQ=255) aaGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGAc > 3:97561/1‑148 (MQ=255) gCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGt < 1:135944/149‑1 (MQ=255) gCTCCCACAGGCGATCGCGGTC‑CTTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGAc < 7:43984/142‑1 (MQ=38) gCTCCCACAGGCGATCGCGGTC‑CTTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGAc > 8:43984/1‑142 (MQ=38) cccACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGcc > 4:227274/1‑148 (MQ=255) acaAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGCCCAGTTGGCCGAgc > 4:5855/1‑149 (MQ=255) g‑gACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGCCGAGCGCtt > 4:25243/1‑149 (MQ=255) gACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑ACCCGTGACCAACGCCCTGACCCATTTGCCGCGCCCCCGGGCCGGCGCCGCCCAGTTGGCCGAGCGCtt > 7:239487/1‑148 (MQ=255) | GGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCACAAG‑GACCGCGATCGCCTGTGGGAGCGGCCCCAGAATCGACCGCAATACCTGTCGCCTGCACCAAAAAAAATAGAGAGGCTAC‑AACCGTGACCAACGCCCTGAACCATTTGCCGCGCCCCCGGGCCGGCGCCGACCAGTTGGCCGAGCGCTT > NC_002947/4549035‑4549328 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |