Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,599,290 G→C 10.2% L173L (CTC→CTG)  PP_4071 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,599,2900GC10.2% 175.0 / 8.9 59L173L (CTC→CTG) PP_4071membrane protein
Reads supporting (aligned to +/- strand):  ref base G (27/26);  new base C (2/4);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

TTTGGCTTGGGTTCCAGACGGTACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACGGCAATCGGTACAGCGCGCGACGC  >  NC_002947/4599143‑4599437
                                                                                                                                                   |                                                                                                                                                   
tttGGCTTGGGTTCCAGACGGTACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGa                                                                                                                                                    <  5:137398/149‑1 (MQ=255)
      ttGGGTTCCAGACGGTACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGCCGGCCAgcgcag                                                                                                                                              >  5:83054/1‑147 (MQ=255)
          ggtCCAGACGGTACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTgcgc                                                                                                                                          <  7:89944/147‑1 (MQ=255)
          gTTCCAGACGGTACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTgcgc                                                                                                                                          <  6:86367/149‑1 (MQ=255)
                   ggTACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGCCGGCCAGCGCAGGCGCTcgcctacc                                                                                                                                 <  3:192300/149‑5 (MQ=255)
                            gACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCCAGCGCAGGCGCTCGCCTACCCCGTggggt                                                                                                                        <  1:242139/149‑5 (MQ=38)
                               tCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAgc                                                                                                                                                  <  4:119520/120‑1 (MQ=255)
                               tCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAgc                                                                                                                                                  >  3:119520/1‑120 (MQ=255)
                               tCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTgcgc                                                                                                                     >  3:249823/1‑149 (MQ=255)
                                 aaCGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTgcgc                                                                                                                     <  4:249823/147‑1 (MQ=255)
                                 aaCGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGCCGGCCAGCGCAGGCGCTCGCCTACCCCGTggggtggt                                                                                                                     <  6:83054/147‑8 (MQ=37)
                                 aaCGTCAGTGGAGTAACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCt                                                                                                                   >  8:19461/1‑149 (MQ=255)
                                            aGTCACTGCCCAGCGAATCTGCGTGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCgg                                                                                                        <  4:57659/149‑1 (MQ=255)
                                            aGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCgg                                                                                                        <  3:53400/149‑1 (MQ=255)
                                             gTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCggg                                                                                                       >  1:19757/1‑149 (MQ=255)
                                             ctCACTGCCCAGCGAATCTGCGGGGCCCTTCTGGCTGAAAAAGGCACCGGGCGGCACGATGATCCACCCATCATTGCAGATCCACCACACCACCGGGTAGGCGAGCGACTGCGCTGTCCGGCCCCGTGCCTGCGCCTAGTCGATGccg                                                                                                        >  6:202920/2‑146 (MQ=255)
                                                aCTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAgc                                                                                                                                                  >  8:218849/1‑103 (MQ=255)
                                                aCTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAgc                                                                                                                                                  <  7:218849/103‑1 (MQ=255)
                                                aCTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCgggac                                                                                                     >  5:161837/1‑146 (MQ=255)
                                                 cTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGt                                                                                                                >  5:63017/1‑136 (MQ=255)
                                                 cTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGt                                                                                                                <  6:63017/136‑1 (MQ=255)
                                                   gCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTg                                                                                                                                        >  3:239947/1‑110 (MQ=255)
                                                   gCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTg                                                                                                                                        <  4:239947/110‑1 (MQ=255)
                                                    cccAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTc                                                                                                               >  1:110526/1‑134 (MQ=255)
                                                    cccAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTc                                                                                                               <  2:110526/134‑1 (MQ=255)
                                                    cccAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAAc                                                                                                  <  2:19757/147‑1 (MQ=255)
                                                     ccAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAAc                                                                                                  >  4:172485/1‑146 (MQ=255)
                                                     ccAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAAc                                                                                                  >  5:223360/1‑146 (MQ=255)
                                                        gCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGaaa                                                                                            <  6:150336/149‑1 (MQ=255)
                                                                     gCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTg                                                                                >  8:25676/1‑148 (MQ=255)
                                                                                tGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAg                                                                    >  8:144302/1‑149 (MQ=255)
                                                                                 gATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGt                                                                                 <  8:155752/135‑1 (MQ=255)
                                                                                 gATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGt                                                                                 >  7:155752/1‑135 (MQ=255)
                                                                                 gATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAgc                                                                   >  5:205303/1‑149 (MQ=255)
                                                                                  aTAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAgct                                                                  >  4:103022/1‑149 (MQ=255)
                                                                                          aCCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGt                                                                            <  8:246379/131‑1 (MQ=255)
                                                                                          aCCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGt                                                                            >  7:246379/1‑131 (MQ=255)
                                                                                             gggcgggTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCgg                                                       >  1:53775/1‑149 (MQ=255)
                                                                                                  ggTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGgcctgc                                                  >  4:228984/1‑149 (MQ=255)
                                                                                                       atgatCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGt                                                                                                      <  7:86296/92‑1 (MQ=255)
                                                                                                       atgatCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGt                                                                                                      >  8:86296/1‑92 (MQ=255)
                                                                                                          atCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCTGGACAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGgcgc                                           <  6:161837/148‑1 (MQ=255)
                                                                                                          atCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGcc                                          >  5:79968/1‑149 (MQ=255)
                                                                                                          atCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGcc                                          <  6:79968/149‑1 (MQ=255)
                                                                                                             aCCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAg                                       >  8:83229/1‑149 (MQ=255)
                                                                                                               cGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGTGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCa                                     >  8:89892/1‑149 (MQ=255)
                                                                                                               cGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCa                                     <  6:223360/149‑1 (MQ=255)
                                                                                                                  atcatTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGTGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGa                                  <  7:89892/149‑1 (MQ=255)
                                                                                                                     atTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAgg                                <  3:228984/148‑1 (MQ=255)
                                                                                                                     atTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGt                               <  2:53775/149‑1 (MQ=255)
                                                                                                                      ttGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATa                                                                       <  6:137047/108‑1 (MQ=255)
                                                                                                                            aTCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACgg                        <  3:172485/149‑1 (MQ=255)
                                                                                                                                  caccCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACGGCAATCg                  >  3:19191/1‑149 (MQ=255)
                                                                                                                                    ccccACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACGGCAATCGGt                <  3:103022/149‑1 (MQ=255)
                                                                                                                                          ggggccggCCAGCGCAGGCGCTCGCCGGCCCCGTGCCCGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGa                                  <  1:4915/119‑1 (MQ=255)
                                                                                                                                          ggggccggCCAGCGCAGGCGCTCGCCGGCCCCGTGCCAGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGa                                  >  2:4915/7‑125 (MQ=255)
                                                                                                                                              tAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACGGCAAt                    >  2:174147/1‑135 (MQ=255)
                                                                                                                                              tAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACGGCAAt                    <  1:174147/135‑1 (MQ=255)
                                                                                                                                                  cGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACGGCAATCGGTACAGCGCGCGACGc  >  1:99721/1‑149 (MQ=255)
                                                                                                                                                   gAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGc                                      >  6:43573/1‑112 (MQ=255)
                                                                                                                                                   gAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGc                                      <  5:43573/112‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTTGGCTTGGGTTCCAGACGGTACCGAAGACTCAACGTCAGTGGAGTCACTGCCCAGCGAATCTGCGGGGCCGTTCTGGCTGATAAAGGCACCGGGCGGGTCGATGATCACCGCATCATTGCTGATCCACCACCCCACGGGGTAGGCGAGCGCCTGCGCTGGCCGGCCCCGTGCCTGCGCCTTGTCGATGCGGGTCAACGAGAAACTTTGATCGGTGCAGTCAATAAAGCTGCTCTTGCCGGCCTGCCTGGCGCCCAGCACGAGGTACCACGGCAATCGGTACAGCGCGCGACGC  >  NC_002947/4599143‑4599437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑