Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,669,794 C→T 7.2% T42T (ACC→ACT)  nuoN → NADH‑quinone oxidoreductase subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,669,7940CT 7.2% 160.4 / 2.2 55T42T (ACC→ACT) nuoNNADH‑quinone oxidoreductase subunit N
Reads supporting (aligned to +/- strand):  ref base C (24/27);  new base T (2/2);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.84e-01

CGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCTGCCTGTACATGGCGATCATCCTCGTGGCGACGCTGGCCTGCGTCACCCTC  >  NC_002947/4669651‑4669938
                                                                                                                                               |                                                                                                                                                
cGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTggg                                                                                                                                             >  8:84333/1‑149 (MQ=255)
   cccGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCt                                                                                                                                          <  3:109621/149‑1 (MQ=255)
    ccGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCt                                                                                                                                          >  3:191669/1‑148 (MQ=255)
     cGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTg                                                                                                                                         >  5:175084/1‑148 (MQ=255)
           gagCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGaa                                                                                                                                       <  1:21081/144‑1 (MQ=255)
           gagCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGaa                                                                                                                                       >  2:21081/1‑144 (MQ=255)
            agCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGc                                                                                                                                 <  6:160190/149‑1 (MQ=255)
            agCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGc                                                                                                                                 >  5:160190/1‑149 (MQ=255)
              cgcTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGccc                                                                                                                               <  7:84333/149‑1 (MQ=255)
               gcTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCct                                                                                                                              <  8:77531/149‑1 (MQ=255)
                       aTTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCg                                                                                                                                                 >  6:223140/1‑122 (MQ=255)
                       aTTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCg                                                                                                                                                 <  5:223140/122‑1 (MQ=255)
                           accacTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCt                                                                                                                  <  3:261827/149‑1 (MQ=255)
                               cTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTc                                                                                                                    >  1:1279/1‑143 (MQ=255)
                               cTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTc                                                                                                                    <  2:1279/143‑1 (MQ=255)
                                  aaCACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGgcg                                                                                                           <  4:209181/149‑1 (MQ=255)
                                  aaCACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGgcg                                                                                                           >  2:186695/1‑149 (MQ=255)
                                       ttCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCt                                                                                                                     <  2:194323/134‑1 (MQ=255)
                                       ttCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCt                                                                                                                     >  1:194323/1‑134 (MQ=255)
                                           tCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACTGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTc                                                                                                  >  7:25619/1‑149 (MQ=255)
                                               aTTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGAGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGCCGTTCCTGCTGTCCACCGTCGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGCAGGTcgccc                                                                                              >  8:180673/1‑147 (MQ=255)
                                                   gcgcCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACTGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCt                                                                                          <  8:25619/149‑1 (MQ=255)
                                                     gcCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTgg                                                                                        <  7:119723/149‑1 (MQ=255)
                                                     gcCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTgg                                                                                        <  7:225431/149‑1 (MQ=255)
                                                       cGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCg                                                                                      <  4:191669/149‑1 (MQ=255)
                                                       cGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCg                                                                                      <  2:181006/149‑1 (MQ=255)
                                                           gCTGATCAGCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTc                                                                                   <  6:125106/148‑1 (MQ=255)
                                                            cTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGTCCTGAACCTg                                                                                                                                   <  5:13965/99‑1 (MQ=255)
                                                            cTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTg                                                                                                                                   >  6:13965/1‑99 (MQ=255)
                                                              gATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACAt                                                                               >  5:270269/1‑149 (MQ=255)
                                                                    caccaTCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGa                                                                         >  7:44239/1‑149 (MQ=255)
                                                                     accaTCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGAt                                                                        >  1:155746/1‑149 (MQ=255)
                                                                             cacGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCctg                                                                                                                             <  2:125318/88‑1 (MQ=255)
                                                                             cacGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATc                                                                 >  2:263062/1‑148 (MQ=255)
                                                                              acGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGa                                                               >  3:119282/1‑149 (MQ=255)
                                                                                     tggtgATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTc                                                        <  6:270269/149‑1 (MQ=255)
                                                                                                  gATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCTGCCTGTACa                                           <  4:119282/149‑1 (MQ=255)
                                                                                                         tGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCTGCCTGTACATGGCGat                                    >  5:80073/1‑149 (MQ=255)
                                                                                                             aGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCCCTGATCACCATCGACAAGTTCGCCTGCCTGTACATGGCGatcatc                                <  1:190161/149‑1 (MQ=255)
                                                                                                                gcAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCgcctgc                                                  >  7:123534/1‑128 (MQ=255)
                                                                                                                gcAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCgcctgc                                                  <  8:123534/128‑1 (MQ=255)
                                                                                                                  aaCCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCTGCCTGTACATGGCGATCATCCTCGt                           >  8:198345/1‑149 (MQ=255)
                                                                                                                    ccACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCTGCCTGTACATGGCGATCATCCTCGTgg                         <  1:186695/149‑1 (MQ=255)
                                                                                                                     cACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCTGCCTGTACATGGCGATCATCCTCGTGGc                        <  3:238024/149‑1 (MQ=255)
                                                                                                                       cTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCAt                                                                  >  5:140283/1‑105 (MQ=255)
                                                                                                                       cTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCAt                                                                  <  6:140283/105‑1 (MQ=255)
                                                                                                                          gCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCTGCCTGTACATGGCGATCATCCTCGTGGCGACGc                   >  8:95554/1‑149 (MQ=255)
                                                                                                                                cTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCTGCCTGTACATGGCGATCATCCTCGTGGc                        >  3:188349/1‑138 (MQ=255)
                                                                                                                                cTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCTGCCTGTACATGGCGATCATCCTCGTGGc                        <  4:188349/138‑1 (MQ=255)
                                                                                                                                   ccTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCTGCCTGTACATGGCGATCATCCTCGTGGCGACGCTGGCCTGc           <  7:95554/148‑1 (MQ=255)
                                                                                                                                      gctgTCCACTGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCTGCCTGTACATGGCGATCATCCTCGTGGCGACGCTGGCCTg            >  8:211850/1‑144 (MQ=255)
                                                                                                                                      gctgTCCACTGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCTGCCTGTACATGGCGATCATCCTCGTGGCGACGCTGGCCTg            <  7:211850/144‑1 (MQ=255)
                                                                                                                                          tCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCTGCCTGTACATGGCGATCATCCTCGTGGCGACGCTGGCCTGCGTCACCCt   >  5:172739/1‑149 (MQ=255)
                                                                                                                                           ccACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCTGCCTGTACATGGCGATCATCCTCGTGGCGACGCTGGCCTGCGTCACCCTc  >  2:240579/1‑149 (MQ=255)
                                                                                                                                            cACCGTGGTCCTGAACCTGGCCCTGCTGGCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCTGCCTGTACATGGCGATCATCCTCGTGGCGACGCTGGCCTGCGTCACCCTc  <  4:169831/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCTGCCTGTACATGGCGATCATCCTCGTGGCGACGCTGGCCTGCGTCACCCTC  >  NC_002947/4669651‑4669938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑