| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,688,071 | C→G | 19.5% | R349R (CGC→CGG) | PP_4148 → | ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,688,071 | 0 | C | G | 19.5% | 57.3 / 4.9 | 25 | R349R (CGC→CGG) | PP_4148 | ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base C (12/8); new base G (2/3); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCCTCGACGGCCTGGGCCTGATGAGTTTCGAAGCGGCCATCAACCGCGACTACCCGGTGGTCTTCGGCACCCTGTTCATCTTCACCCTGCTAGGGCTGGTGGTGAAACTGATCGGCGACCTGACCTACACCTTGGTCGATCCGCGCATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCCGGCGCTTCGAGCGTTTCAAGGCCAACCGCCGTGGCTGGTGGTCGCTGTGGCTGTTCCTGATTCTGTTCGGGCTGAGCCTGGGCGCCGAA > NC_002947/4687925‑4688213 | aGCCTCGACGGCCTGGGCCTGATGAGTTTCGAAGCGGCCATCAACCGCGACTACCCGGTGGTCTTCGGCACCCTGTTCATCTTCACCCTGCTAGGGCTGGTGGTGAAACTGATCGGCGACCTGACCTACACCTTGGTCGATCCGCGCAt > 4:252726/1‑149 (MQ=255) cGGCCTGGGCCTGATGAGTTTCGAAGCGGCCATCAACCGCGACTACCCGGTGGTCTTCGGCACCCTGTTCATCTTCACCCTGCTAGGGCTGGTGGTGAAACTGATCGGCGACCTGACCTACACCTTGGTCGATCCGCGCATCGACTTTg < 3:252726/149‑1 (MQ=255) ccGGTGGTCTTCGGCACCCTGTTCATCTTCACCCTGCTAGGGCTGGTGGTGAAACTGATCGGCGACCTGACCTACACCTTGGTCGATCCGCGCATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCCGgc > 1:76541/1‑149 (MQ=255) cACCCTGTTCATCTTCACCCTGCTAGGGCTGGTGGTGAAACTGATCGGCGACCTGACCTACACCTTGGTCGATGCGCGGATCGAcca > 6:54611/1‑85 (MQ=255) cACCCTGTTCATCTTCACCCTGCTAGGGCTGGTGGTGAAACTGATCGGCGACCTGACCTACACCTTGGTCGATGCGCGGATCGAcca < 5:54611/87‑3 (MQ=255) gTTCATCTTCACCCTGCTAGGGCTGCTGCAGAAACTGATCGGCGACATGACCTACACCTTGCTCGATCCGCGCATCGACTTAGCCAGCCGGGACCACAGACATGCCCATGTCCCCCCTCAACCCCCGGCGCTTCGAGCGATTCAAgcc > 4:114984/1‑146 (MQ=255) aTCTTCACCCTGCTAGGGCTGGTGGTGAAACTGATCGGCGACCTGACCTACACCTTGGTCGATCCGCGCATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCCGGCGCTTCGAGCGTTTCAAGGCCAAcc > 3:37358/1‑149 (MQ=255) gggCTGGTGGTGAAACTGATCGGCGACCTGACCTACACCTTGGTCGATCCGCGCATCGACTTTGCGAGCGGGGAGCACTGACATGGCGTTGTCCCCCCTGAACCGCCGGCGCTTCGAGCGTTTCAAGGCCAACCGCCGTGGCtggtg < 3:114984/147‑1 (MQ=255) gggCTGGTGGTGAAACTGATCGGCGACCTGACCTACACCTTGGTCGATCCGCGCATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCCGGCGCTTCGAGCGGTTCAAGGCCAACCGCCGTGGCtggtg < 7:81492/147‑1 (MQ=255) cTGATCGGCGACCTGACCTACACCTTGGTCGATCCGCGCATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCCGGCGCTTCGAGCGTTTCAAGGCCAACCGCCGTGGCTGGTGGTCGCTGTGGCTGTTcc < 8:70100/149‑1 (MQ=255) tGATCGGCGACCTGACCTACACCTTGGTCGATCCGCGCATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCCGGCGCTTCGAGCGTTTCAAGGCCAACCGCCGTGGCTGGTGGTCGCTGTGGCTGTTCCt > 2:261350/1‑149 (MQ=255) ggCGACCTGACCTACACCTTGGTCGATCCGCGCATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCCGGCGCTTCGAGCGTTTCAAGGCCAACCGCCGTGGCTGGTGGTCGCTGTGGCTGTTcc > 5:257294/1‑143 (MQ=255) gacctACACCTTGGTGGATCCGCGGATCGACTTTGCCAGCCGGGAGGACTGACATGGCCTTGTCCGCCGTCAACCGCCGGCGCTTCGAGCGTTTc < 4:181813/95‑1 (MQ=255) gacctACACCTTGGTCGATCCGCGCATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCCGGCGCTTCGAGCGTTTCAAGGCCAACCGCCGTGGCTGGTGGTCGCTGTGGCTGTTCCTGATTCTGTTCggg > 2:31786/1‑149 (MQ=255) gacctACACCTTGGTCGATCCGCGCATCGACTTTGACAGCCGGGAGCACTGACATCGCCTTGTCCCCACTCAACCGCCGGCGCTTCGAGCGTTTc > 3:181813/1‑95 (MQ=255) acctACACCTTGGTCGATCCGCGCATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCCGGCGCTTCGAGCGTTTCAAGGCCAACCGCCGTGGCTGGTGGTCGCTGTGGCTGTTCCTGATTCTGTTCggg < 3:55387/148‑1 (MQ=255) tACACCTTGGTCGATCCGCGCATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCCGGCGCTTCGAGCGTTTCAAGGCCAACCGCCGTGGCtggtg < 4:112908/114‑1 (MQ=255) tACACCTTGGTCGATCCGCGCATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCCGGCGCTTCGAGCGTTTCAAGGCCAACCGCCGTGGCtggtg > 3:112908/1‑114 (MQ=255) acacCTTGGTCGATCCGCGCATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCCGGCGCTTCGAGCGTTTCAAGGCCAACCGCCGTGGCTGGTGGTCGCTGTGGCTGTTCCTGATTCTGTTCGGGCTGAg > 3:76027/1‑149 (MQ=255) ttGGTCGATCCGCGCATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCTGGCGCTTCGAGCGTTTCAAGGCCAACCGCCGTGGCTGGTGGTCGCTGTGGCTGTTc < 7:22072/124‑1 (MQ=255) ttGGTCGATCCGCGCATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCTGGCGCTTCGAGCGTTTCAAGGCCAACCGCCGTGGCTGGTGGTCGCTGTGGCTGTTc > 8:22072/1‑124 (MQ=255) aaagTCGATGCGCGGATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCCGGCGCTTCGAGCGTTTCAAGGCCAACCGCCGTGGCTGGTGGTCGCTGTGGCTGTTCCTGATTCTGTTCGGGCTGAGCCTgg > 2:176671/4‑149 (MQ=255) tCGATCCGCGCATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCCGGCGCTTCGAGCGTTTCAAGGCCAACCGCCGTGGCTGGTGGTCGCTGTGGCTGTTCCTGATTCTGTTCGGGCTGAGCCTGGgcgc < 1:31786/149‑1 (MQ=255) tgcgcgGATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCCGGCGCTTCGAGCGTTTCAAGGCCAACCGCCGTGGCTGGTGGTCGCTGTGGCTGTTCCTGATTCTGTTCGGGCTGAGCCTGGGCGCCGaa < 1:176671/147‑1 (MQ=255) tCCGCGCATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCCGGCGCTTCGAGCGTTTCAAGGCCAACCGCCGTGGCTGGTGGTCGCTGTGGCTGTTCCTGATTCTGTTCGGGCTGAGCCTGGGCGCCGaa > 2:59259/1‑149 (MQ=255) | AGCCTCGACGGCCTGGGCCTGATGAGTTTCGAAGCGGCCATCAACCGCGACTACCCGGTGGTCTTCGGCACCCTGTTCATCTTCACCCTGCTAGGGCTGGTGGTGAAACTGATCGGCGACCTGACCTACACCTTGGTCGATCCGCGCATCGACTTTGCCAGCCGGGAGCACTGACATGGCCTTGTCCCCCCTCAACCGCCGGCGCTTCGAGCGTTTCAAGGCCAACCGCCGTGGCTGGTGGTCGCTGTGGCTGTTCCTGATTCTGTTCGGGCTGAGCCTGGGCGCCGAA > NC_002947/4687925‑4688213 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |