| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,705,413 | T→A | 20.7% | intergenic (‑151/+26) | kdpF ← / ← PP_4162 | potassium‑transporting ATPase subunit F/membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,705,413 | 0 | T | A | 20.7% | 56.8 / 8.7 | 29 | intergenic (‑151/+26) | kdpF/PP_4162 | potassium‑transporting ATPase subunit F/membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (17/6); new base A (4/2); total (21/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATCTCCCCGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGC > NC_002947/4705268‑4705557 | gATCTCCCCGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGc > 3:99606/1‑149 (MQ=255) ccccGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTAGCCGGCTTGCCcgc > 6:41686/1‑148 (MQ=255) ggAAATTGTCCGAGCGAGAGGCGAAAAGGGGCGAGATCGGCGGGTGCGCGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCGTAATGTTGTG > 8:184784‑M1/1‑149 (MQ=255) gAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGG > 7:67858‑M1/1‑149 (MQ=255) gAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTCCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAggg > 1:68323/1‑126 (MQ=255) ggggggTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCcagcag > 7:80645/1‑148 (MQ=255) gAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGtaggctacagcctgctgctggactggagccgtaacgcggcgcagcagccgccacaa < 8:67858‑M1/149‑57 (MQ=255) aaaaaTCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGc > 7:198323/1‑149 (MQ=255) aaaTCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGccc > 3:144377/1‑149 (MQ=255) tCAGCGTTGTGGTCTGCCTGTAACCGCCTCTTCGCGGGCTAGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTTCGCCGCGTTACGGCTCCAGTCCCGCAGCATGCTGTAGCCTCCCGCCAGCCAGGTCGGCCCGAt > 5:7277/1‑149 (MQ=255) tgGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGcc < 8:80645/149‑1 (MQ=255) tCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGt < 7:177979/109‑1 (MQ=255) tCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGt > 8:177979/1‑109 (MQ=255) tCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAAt > 1:15338/1‑149 (MQ=255) gTAACGGCCCTTTCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGAGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCAACCGCCAGCAAGGTCGGCCCGATGAAAAGGACAATGAAGcc > 6:176798/1‑148 (MQ=37) aCGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGaaaa > 7:120640/1‑132 (MQ=255) aCGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGaaaa < 8:120640/132‑1 (MQ=255) ggccctttCGCGGGCAAGCCCGCGAAGGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAgg > 7:100394/7‑148 (MQ=39) ggCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgc < 2:15338/149‑1 (MQ=255) gCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgc > 5:107319/1‑148 (MQ=255) ccctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgc > 1:206082/5‑148 (MQ=38) tctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaag > 2:102416/1‑148 (MQ=255) ttCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGtgggctacagcctgctgctggactggagccgtaacgcggcgcagcagccgccacaacaa > 1:244722‑M1/1‑50 (MQ=255) ttCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGtgggctacagcctgctgctggactggagccgtaacgcggcgcagcagccgccacaacaa < 2:244722‑M1/109‑60 (MQ=255) tCGCGGGCAAGCCCGCGAAGGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaagcaa < 8:100394/148‑1 (MQ=255) cgcgGGCAAGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCcagca < 1:238669/80‑1 (MQ=255) cgcgGGCAAGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCcagca > 2:238669/1‑80 (MQ=255) cgGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAgccg > 3:57229/1‑149 (MQ=255) cggCTTGCCCGCGAAAGGTCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGTCTCCAGAACAGCAGCATGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATTAAAACGCCAATGAATCCAAATGCAAGCAAgccgc > 2:101314/2‑149 (MQ=255) | GATCTCCCCGTGTTACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGC‑GGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGC > NC_002947/4705268‑4705557 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |