| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,725,041 | A→T | 11.3% | intergenic (+26/+42) | PP_5661 → / ← PP_4183 | putative lipoprotein/membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,725,041 | 0 | A | T | 11.3% | 166.5 / 6.9 | 60 | intergenic (+26/+42) | PP_5661/PP_4183 | putative lipoprotein/membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (26/27); new base T (2/5); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGAGGAAAACGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATCGAGTACATCCGAATGGAACTCTCGACG > NC_002947/4724900‑4725184 | ccgAGGAAAACGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCggg > 1:104303/1‑149 (MQ=255) aGGAAAACGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACATCGTCGGGttt < 7:56400/149‑1 (MQ=255) aaaCGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAATCCCGACATCGTCGGGttttttt < 7:169559/149‑1 (MQ=255) aaaCGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGttttttt < 1:163520/149‑1 (MQ=255) aaaCGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACATCGTCGGGttttttt < 5:271800/149‑1 (MQ=255) aaaCGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACATCGTCGGGttttttt < 2:171449/149‑1 (MQ=255) aaCGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGATGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTtg < 2:10874/149‑1 (MQ=255) ttcTGCCAAAGATCAGGCCGAAGCCTAGCAGAAGGCTGCCTAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGc < 8:233977/146‑1 (MQ=255) tGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACATCGTCGGGttttttt < 4:166407/137‑1 (MQ=255) tGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACATCGTCGGGttttttt > 3:166407/1‑137 (MQ=255) ccAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACtt > 7:132523/1‑149 (MQ=255) ccAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACtt > 6:32539/1‑149 (MQ=255) aaaGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTc < 2:194815/149‑1 (MQ=255) aGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAACACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGa < 3:167223/149‑1 (MQ=255) aGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTaa > 4:167103/1‑148 (MQ=255) gCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCg > 8:44092/1‑149 (MQ=255) gAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGttt < 2:104303/149‑1 (MQ=255) ccGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGt > 4:121917/1‑149 (MQ=255) ccGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGa < 5:32539/147‑1 (MQ=255) ccGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGa < 4:87488/147‑1 (MQ=255) ccGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGc > 5:81873/1‑149 (MQ=255) agCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCtt > 7:68508/1‑149 (MQ=255) cGCTCCGGCTACTCCGGCTCCTACTACACCGCCAGAACCTCCCAAGCAGTAATTGGCTAAAAAAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGc < 1:268761/149‑1 (MQ=255) tCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCgg > 7:149237/1‑149 (MQ=255) cGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCt > 5:12825/1‑149 (MQ=255) gCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTc > 2:57150/1‑148 (MQ=255) gCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTc < 1:57150/148‑1 (MQ=255) cTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTcc > 4:224009/1‑148 (MQ=255) cTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTcc < 3:123309/133‑1 (MQ=255) cTCCGGATCCTACTACACCGGCAGAACCTGACAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTcc > 4:123309/1‑133 (MQ=255) ctactaCACCGGCAGAACCTTACAAGCTGTAATTTGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACttt < 6:12825/149‑1 (MQ=255) ctacACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTtcgg > 6:19102/1‑149 (MQ=255) ctacACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTtc < 8:132523/147‑1 (MQ=255) tacACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGt > 1:198092/1‑138 (MQ=255) tacACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGt < 2:198092/138‑1 (MQ=255) tacACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTaca < 1:82418/141‑1 (MQ=255) tacACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTaca > 2:82418/1‑141 (MQ=255) acacCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTtcggtc < 7:44092/149‑1 (MQ=255) cacCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTaca < 3:101614/139‑1 (MQ=255) cacCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTaca > 4:101614/1‑139 (MQ=255) cGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGgtg < 3:167103/149‑1 (MQ=255) cAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGa < 7:69950/149‑1 (MQ=255) cAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGa < 5:19102/149‑1 (MQ=255) aGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGaa < 6:81873/149‑1 (MQ=255) gAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGtgtg > 5:76393/1‑146 (MQ=255) gAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGtgtg < 6:76393/146‑1 (MQ=255) ccTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGcc < 6:150957/113‑1 (MQ=255) ccTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGcc > 5:150957/1‑113 (MQ=255) cTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACAcc > 3:10063/1‑148 (MQ=255) gCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATc > 3:62051/1‑149 (MQ=255) aGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATc > 7:62926/1‑147 (MQ=255) gTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATCGAg > 2:163624/1‑149 (MQ=255) aaTTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCGTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACtt < 5:109917/118‑1 (MQ=255) aaTTGGCTAAAACAAAAAAACCAGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTAAGTTTGAAACAACCGAGATTTCCTTGTCCGGCTCCGACAGTAAACtt > 6:109917/1‑118 (MQ=255) gCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATCGAGTACATcc > 6:68044/1‑149 (MQ=255) gCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATCGAGTACATcc < 8:62926/149‑1 (MQ=255) gCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATCGAGTACATc < 3:224009/148‑1 (MQ=255) aaaaaaaCCCGACATCGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTAAACTTTCGGTCGGTGTGAACACCATCACATCGAGTACATCCGAATGGaa > 2:153085/1‑149 (MQ=255) ccGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGa < 6:235445/110‑1 (MQ=255) ccGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGa > 5:235445/1‑110 (MQ=255) cgacgaTGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATCGAGTACATCCGAATGGAACTCTCGACg < 5:68044/149‑1 (MQ=255) | CCGAGGAAAACGCCGAAGCTGCCAAAGATCAGGCCGAAGCCGAGCAGAAGGCTGCCGAAGAAGCCGCTCCGGCTACTCCGGCTCCTACTACACCGGCAGAACCTGCCAAGCAGTAATTGGCTAAAACAAAAAAACCCGACGATGTCGGGTTTTTTTGTGCCTGCGAACTTTCGATTAATCGTTTCAGTTGGAAACAACCGAGCTTTCCTTGGCCGGCTCCGACAGTACACTTTCGGTCGGTGTGAACACCATCACATCGAGTACATCCGAATGGAACTCTCGACG > NC_002947/4724900‑4725184 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |