Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 344,140 T→G 8.2% noncoding (36/76 nt) PP_t72 ← tRNA‑Phe

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947344,1400TG 8.2% 142.3 / 4.8 49noncoding (36/76 nt)PP_t72tRNA‑Phe
Reads supporting (aligned to +/- strand):  ref base T (23/22);  new base G (2/2);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

TCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAA‑CAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGTCATTCGGCCGGTGGAACG  >  NC_002947/343993‑344284
                                                                                                                                                     |                                                                                                                                                
tcttcGACGCCGCCTGAAGTTAAAAATCACAGACAATCAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGAttt                                                                                                                                                  >  1:180528/1‑149 (MQ=255)
 cttcGACGCCGCCTGAAGTTAAAAATCACAGACAA‑CAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGAtttt                                                                                                                                                 <  7:8669/148‑1 (MQ=255)
 cttcGACGCCGCCTGAAGTTAAAAATCACAGACAA‑CAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGAtttt                                                                                                                                                 <  6:14505/148‑1 (MQ=255)
    cGACGCCGCCTGAAGTTAAAAATCACAGACAA‑CAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCaa                                                                                                                                              <  1:9041/148‑1 (MQ=255)
    cGACGCCGCCTGAAGTTAAAAATCACAGACAA‑CAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCa                                                                                                                                               <  6:71907/147‑1 (MQ=255)
          cgccTGAAGTTAAAAATCACAGACAA‑CAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTGAAAATCctcgt                                                                                                                                       <  8:62154/149‑3 (MQ=255)
          cgccTGAAGTTAAAAATCACAGACAA‑CAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTGAAAATCctc                                                                                                                                         <  6:184467/147‑1 (MQ=255)
          cgccTGAAGTTAAAAATCACAGACAA‑CAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTGAAAATCctc                                                                                                                                         >  5:184467/1‑147 (MQ=255)
               gAAGTTAAAAATCACAGACAA‑CAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTa                                                                                                                                  >  5:131050/1‑149 (MQ=255)
                aaGTTAAAAATCACAGACAA‑CAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTAc                                                                                                                                 <  2:238369/149‑1 (MQ=255)
                aaGTTAAAAATCACAGACAA‑CAAAAAACACTCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTa                                                                                                                                  <  7:187877/148‑1 (MQ=255)
                     aaaaaTCACAGACAA‑CAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACt                                                                                                                            >  1:250893/1‑149 (MQ=255)
                                 caa‑caaAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGTTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTggg                                                                                                                <  2:250893/149‑1 (MQ=255)
                                 caa‑caaAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTggg                                                                                                                <  2:180528/149‑1 (MQ=255)
                                  aa‑caaAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGc                                                                                                               >  8:220207/1‑149 (MQ=255)
                                     caaAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTg                                                                                                                           <  1:231712/135‑1 (MQ=255)
                                     caaAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTg                                                                                                                           >  2:231712/1‑135 (MQ=255)
                                      aagaaaCCCGC‑CTAGTGCGGGTTTTTTGTTGTCGCTTCGGGTAACTTTGCAACCACGAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGcgac                                                                                                            >  2:209311/4‑149 (MQ=255)
                                      aagaaaCCCGC‑CTAGTGCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACAACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTACAATCCTCTGATCTACCGACTGAGCTATCTGGGcgac                                                                                                            >  6:76130/4‑149 (MQ=255)
                                       aaaaaCCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGcgacg                                                                                                           <  2:36335/149‑1 (MQ=255)
                                             ccGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCTCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTg                                                                                                      >  5:12312/1‑148 (MQ=255)
                                             ccGCACTAG‑GCGCGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTg                                                                                                      >  7:208431/1‑148 (MQ=255)
                                                   tAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTAt                                                                                                                     <  7:4115/127‑1 (MQ=255)
                                                   tAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTAt                                                                                                                     >  8:4115/1‑127 (MQ=255)
                                                            tttCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTc                                                                                       <  4:189449/149‑1 (MQ=255)
                                                                 tgttgtCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATACTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGAc                                                                                  >  7:189891/1‑149 (MQ=255)
                                                                     gtCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGAtttt                                                                                                                                                 <  7:21038/82‑1 (MQ=255)
                                                                     gtCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGAtttt                                                                                                                                                 >  8:21038/1‑82 (MQ=255)
                                                                         cTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAATTAGATTTCCGGACCGCTCGTc                                                                          <  6:131050/149‑1 (MQ=255)
                                                                             gggTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAAc                                                                       <  6:12312/148‑1 (MQ=255)
                                                                              ggTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGa                                                                     <  7:220207/149‑1 (MQ=255)
                                                                                     ttGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTATACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACttttt                                                               >  4:162409/1‑148 (MQ=255)
                                                                                           ccaccaGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTTGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGaaaaaa                                                        >  8:102693/1‑149 (MQ=255)
                                                                                            caccaGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAAt                                                       >  6:190269/1‑149 (MQ=255)
                                                                                              ccaGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACttttt                                                               >  6:126246/1‑139 (MQ=255)
                                                                                              ccaGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACttttt                                                               <  5:126246/139‑1 (MQ=255)
                                                                                                 gAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTa                                                   >  7:144189/1‑148 (MQ=255)
                                                                                                  aaGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTTGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTaaa                                                 <  5:76130/149‑1 (MQ=255)
                                                                                                          aGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTcc                                         <  8:215216/149‑1 (MQ=255)
                                                                                                                  cccAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTa                                 <  8:144189/149‑1 (MQ=255)
                                                                                                                          cGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGAttttt                         <  8:208431/149‑1 (MQ=255)
                                                                                                                              gTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTACGTCGCTTACGATTTTTgggg                     >  7:1096/1‑149 (MQ=255)
                                                                                                                                 gAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCt                                   >  1:175538/1‑132 (MQ=255)
                                                                                                                                 gAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCt                                   <  2:175538/132‑1 (MQ=255)
                                                                                                                                 gAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGTCa                  >  1:194845/1‑149 (MQ=255)
                                                                                                                                            acGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGt                    <  6:53710/136‑1 (MQ=255)
                                                                                                                                            acGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGt                    >  5:53710/1‑136 (MQ=255)
                                                                                                                                             agAGGATTGAAAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCAGTCGCTTACGATTTTTTGGGTCATTCGGCCGGTg       >  1:197936/2‑148 (MQ=255)
                                                                                                                                              gAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGTCATTCGGCCGGTgg      >  3:201654/1‑148 (MQ=255)
                                                                                                                                               aGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGAGCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGTCATTCGGCCGGTGGaa    <  5:190269/149‑1 (MQ=255)
                                                                                                                                                ggATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAAt                                                       <  6:168808/97‑1 (MQ=255)
                                                                                                                                                ggATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAAt                                                       >  5:168808/1‑97 (MQ=255)
                                                                                                                                                 gATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGTCATTCGGCCGGTGGAACg  >  3:117637/1‑149 (MQ=255)
                                                                                                                                                 gATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGTCATTCGGCCGGTGGAACg  >  1:246247/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
TCTTCGACGCCGCCTGAAGTTAAAAATCACAGACAA‑CAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGTCATTCGGCCGGTGGAACG  >  NC_002947/343993‑344284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑