Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,738,564 A→G 7.7% F60L (TTC→CTC)  sdhD ← succinate dehydrogenase hydrophobic membrane anchor subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,738,5640AG 7.7% 146.6 / 2.0 52F60L (TTC→CTC) sdhDsuccinate dehydrogenase hydrophobic membrane anchor subunit
Reads supporting (aligned to +/- strand):  ref base A (22/26);  new base G (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

ACATCGCAACGCCGCATACCGCCTGGAACAGGAAACGAATCGCAGTTGCCGACTTGCCGAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCAGGAAGAGGAAGTAAGCCGCGAGAACGACCGCAGAAACGCGCTGCGCCATCCAGTCATAGAGGC  >  NC_002947/4738417‑4738707
                                                                                                                                                   |                                                                                                                                               
aCATCGCAACGCCGCATACCGCCTGGAACAGGAAACGAATCGTAGTTGCCGACTTGCCGAACGACTTAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTTTGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAg                                                                                                                                                <  4:91238/149‑1 (MQ=255)
     gCAACGCCGCATACCGCCTGGAACAGGAAACGAATCGCAGTTGCCGACTTGCCGAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATcc                                                                                                                                            <  5:92859/148‑1 (MQ=255)
              cATACCGCCTGGAACAGGAAACGAATCGCAGTTGCCGACTTGCCGAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGTCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCgtt                                                                                                                                  <  1:52736/149‑1 (MQ=255)
                         gAACAGGAAACGAATCGCAGTTGCCGACTTGCCGAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCTACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGaa                                                                                                                         <  6:140027/147‑1 (MQ=255)
                         gAACAGGAAACGAATCGCAGTTGCCGACTTGCCGAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAGGATCCGCATCGCGTTGTTGGAGaa                                                                                                                         >  1:259670/1‑147 (MQ=255)
                         gAACAGGAAACGAATCGCAGTTGCCGACTTGCCGAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAGGATCCGCATCGCGTTGTTGGAGaa                                                                                                                         >  1:255608/1‑147 (MQ=255)
                           aCAGTAAACGAATCGCAGTTGCCGACTTGCCGAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGa                                                                                                                     <  3:250690/149‑1 (MQ=255)
                             aGGAAACGAATCGCAGTTGCCGACTTGCCGAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATcgcg                                                                                                                                    <  1:139765/132‑1 (MQ=255)
                             aGGAAACGAATCGCAGTTGCCGACTTGCCGAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATcgcg                                                                                                                                    >  2:139765/1‑132 (MQ=255)
                                 aaCGAATCGCAGTTGCCGACTTGCCGAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGc                                                                                                               <  7:94853/149‑1 (MQ=255)
                                         gCAGTTGCCGACTTGCCGAACGACATAGGCGTCAGGTAGTCGTTTGCAATGGTCCACATGCCGACCCATGCTTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGgt                                                                                                       <  7:58366/149‑1 (MQ=255)
                                          cAGTTGCCGACTTGCCGAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGgtg                                                                                                      >  4:198539/1‑149 (MQ=255)
                                                ccGACTTGCCGAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAGGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTc                                                                                                 <  2:259670/148‑1 (MQ=255)
                                                ccGACTTGCCGAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAGGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTc                                                                                                 <  2:255608/148‑1 (MQ=255)
                                                 cGACTTGCCGAACGACATAGGCGTCAGGTAGGCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGa                                                                                               <  6:91890/149‑1 (MQ=255)
                                                     ttGCCGAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGt                                                                                                  <  5:107190/142‑1 (MQ=255)
                                                     ttGCCGAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGt                                                                                                  >  6:107190/1‑142 (MQ=255)
                                                         cGAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCtggg                                                                                       >  7:53289/1‑149 (MQ=255)
                                                          gAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCtgggt                                                                                      >  4:23719/1‑149 (MQ=255)
                                                          gAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCtgggt                                                                                      <  3:134463/149‑1 (MQ=255)
                                                                     gCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGAc                                                                               <  2:201822/145‑1 (MQ=255)
                                                                     gCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGAc                                                                               >  1:201822/1‑145 (MQ=255)
                                                                          aGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGga                                                                                                                            >  2:181882/1‑95 (MQ=255)
                                                                          aGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGga                                                                                                                            <  1:181882/95‑1 (MQ=255)
                                                                          aGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGACGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGc                                                                       <  2:81706/148‑1 (MQ=255)
                                                                               gTCGGGTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCa                                                                 <  6:218048/149‑1 (MQ=255)
                                                                                tCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGAGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCag                                                                >  1:184335/1‑149 (MQ=255)
                                                                                  ggTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCagg                                                               <  3:198539/148‑1 (MQ=255)
                                                                                        aaTGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCaggaagagg                                                         >  6:202445/1‑148 (MQ=255)
                                                                                        aaTGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCaggaagag                                                          >  6:251582/1‑147 (MQ=255)
                                                                                        aaTGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCaggaagag                                                          <  5:251582/147‑1 (MQ=255)
                                                                                        aaTGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTGGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCaggaagagg                                                         >  2:178053/1‑148 (MQ=255)
                                                                                           ggTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTgg                                                                                                         <  3:216169/97‑1 (MQ=255)
                                                                                           ggTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTgg                                                                                                         >  4:216169/1‑97 (MQ=255)
                                                                                               cacaTGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCAGGAAGAGGAAGTAAGc                                                 >  3:221213/1‑149 (MQ=255)
                                                                                                          ccAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCAGGAAGAGGAAGTAAGCCGCGAGAACGa                                      >  1:137880/1‑149 (MQ=255)
                                                                                                             ggCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCAGGAAGAGGAAGTAAGCCGCGAGAACGACCg                                   <  8:53289/149‑1 (MQ=255)
                                                                                                              gCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCagg                                                               >  4:161728/1‑120 (MQ=255)
                                                                                                              gCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCagg                                                               <  3:161728/120‑1 (MQ=255)
                                                                                                                          aGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCAGGAAGAGGAAGTAAGCCGCGAGAACGACCGCAGAAACGCGCTg                      >  2:122239/1‑149 (MQ=255)
                                                                                                                           gggCAACGAGGGCCAGCAGTCTGAAGATCCGAATCTCGTTGTTGGTGAAAAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTTGCCGATCAGTAAGAGGAAGTAAGGCGCGAGAACGACCGCAGAAACGCGCTgc                     <  4:221213/149‑1 (MQ=255)
                                                                                                                            ggCAACGAGGGCCTGGAGACTGTAGATCCGCATCGCGTTGTTGGATAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCAGGAAGAGGAAGTAAGCCGCGAGAACGACCGCAGAAACGCGCTgcg                    <  3:91931/149‑1 (MQ=255)
                                                                                                                               aaCGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCAGGAAGAGGAAGTAAGCCGCGAGAACGACCGCAGAAACGCGCTGCGCCa                 >  7:58519/1‑149 (MQ=255)
                                                                                                                                   aGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGAt                                                                   >  4:159357/1‑95 (MQ=255)
                                                                                                                                   aGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGAt                                                                   <  3:159357/95‑1 (MQ=255)
                                                                                                                                     ggCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCAGGAAGAGGAAGTAAGCCGCGAGAACGACCGCAGAAACGCGCTGCGCCATCCAGt           >  4:39730/1‑149 (MQ=255)
                                                                                                                                         agcagACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCAGGAAGAGGAAGTAAGCCGCGAGAACGACCGCAGAAACGCGCTGCGCCATCCAGTCATa       <  2:137880/149‑1 (MQ=255)
                                                                                                                                              aCTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCAGGAAGAGGAAGTAAGCCGCGAGAACGACCGCAGAAACGCGCTGCGCCATCCAGTCATAGAGGc  >  7:159914/1‑149 (MQ=255)
                                                                                                                                                tGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCAGGAAGAGGAAGTAAGc                                                 <  5:267551/100‑1 (MQ=255)
                                                                                                                                                tGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCAGGAAGAGGAAGTAAGc                                                 >  6:267551/1‑100 (MQ=255)
                                                                                                                                                 gAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCAGGAAGAGGAAGTAAGCcgcg                                             >  2:250181/1‑103 (MQ=255)
                                                                                                                                                 gAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCAGGAAGAGGAAGTAAGCcgcg                                             <  1:250181/103‑1 (MQ=255)
                                                                                                                                                   aGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCAGGAAGAGGAAGTAAGCCGCGAGAACGACCGCAGAAACGCGCTGCGCCATCCAGTCa         <  6:2294/137‑1 (MQ=255)
                                                                                                                                                   aGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCAGGAAGAGGAAGTAAGCCGCGAGAACGACCGCAGAAACGCGCTGCGCCATCCAGTCa         >  5:2294/1‑137 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
ACATCGCAACGCCGCATACCGCCTGGAACAGGAAACGAATCGCAGTTGCCGACTTGCCGAACGACATAGGCGTCAGGTAGTCGGTTGCAATGGTCCACATGCCGACCCAGGCGTGAGCGCCCAGGGCAACGAGGGCCAGCAGACTGAAGATCCGCATCGCGTTGTTGGAGAACAGACCATGCCACTGGGTGTAGTCGATGCCTGGGTGGGCGACCACGTAGCCGATCAGGAAGAGGAAGTAAGCCGCGAGAACGACCGCAGAAACGCGCTGCGCCATCCAGTCATAGAGGC  >  NC_002947/4738417‑4738707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑