| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,740,816 | T→G | 100% | intergenic (+53/+700) | gltA → / ← yeiW | citrate synthase/putative oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,740,816 | 0 | T | G | 96.6% | 120.3 / ‑6.2 | 40 | intergenic (+53/+700) | gltA/yeiW | citrate synthase/putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (0/3); new base G (22/15); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.26e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTG‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCA > NC_002947/4740682‑4740954 | cccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCctc > 4:150675‑M2/4‑149 (MQ=255) aTCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCt > 5:272339/1‑149 (MQ=255) ccGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGa < 3:5260/149‑1 (MQ=255) cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCcgcg > 1:162942/1‑147 (MQ=255) gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCgggcaaggc < 7:142200/142‑8 (MQ=255) gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAag < 5:276343/149‑1 (MQ=255) gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACAGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCgggcaaggc > 8:142200/1‑135 (MQ=255) gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag > 5:161064/1‑149 (MQ=255) aGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGcc < 2:16883/149‑1 (MQ=255) acacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCgg < 8:44293/124‑1 (MQ=255) cacCGCCGACCAGACCCGCGACATCGTTCCCCTTAAAGCCCCCAAGTACGCTGCATTAGCCGACCGCAAGAAGGCTGCCCTCGGGCTGCC‑TTTTTTATTTGTCCT‑GGGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGTGGCCGGcaca < 4:196404/148‑1 (MQ=255) cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCACAAACCGGCCATGCCCGCCAAGAGGCCGCcacacaca > 1:244794/1‑149 (MQ=255) taaGCGTGACTTCGTTGCCCTTAAATACCGAATTTATGCTGCAGTAGCGTAACGAAATATGGGTGCCCT‑GGGTAAGCTTTTTTTTTTTGTCGT‑GTGCGGGCCTTTGGGGGGCTTGGCCCGCTATGTGGCCGGCACAGACACCACAAAAc < 2:191120/148‑1 (MQ=255) cGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACCGACACCACAAACCCTCAGgttttat > 8:132394/1‑147 (MQ=255) gTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCTAACGCAAGAAGGCTGCCGTCGGG‑ATGCGTTTTTTATTTTTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtct < 7:132394/148‑1 (MQ=255) cccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga > 3:132404/1‑109 (MQ=255) cccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga < 4:132404/109‑1 (MQ=255) cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTTGCCGCGAAGAGGCCGGCACAGAAACCACAAAAACACAGTTTTtctagatag > 7:8265/1‑142 (MQ=255) cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgc > 2:41262/1‑146 (MQ=255) cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgc < 1:41262/146‑1 (MQ=255) tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGCAGAGGCCGGCCCAGCCACCACAAAACCTCAGGTTTTCTccgcccgg > 6:215386/1‑148 (MQ=255) aGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCAGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCAc > 1:115554/1‑149 (MQ=255) gCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGc < 6:272339/149‑1 (MQ=255) gCTGCAGTAGCCTAACGCAAGAAGGCTGCCCGCGGGCAGCC‑TTTTTTATTTTGCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg < 5:91902/149‑1 (MQ=255) cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg < 7:48804/149‑1 (MQ=255) aaCCCAAGACGGCTGCCCTCGGGCAGCCTTTTTTT‑TTTGTCCT‑GTCCCGGCCTGTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgc < 3:27703/149‑1 (MQ=255) aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACAACAGTTTTTATAAGCCCGCTCACGCCGCCCTTTAAg > 2:170877/1‑131 (MQ=37) aaaaagGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGGTTTTCTCCGCCCGCTCCCGCAGCCCTTTAAggg > 7:13920/4‑133 (MQ=37) agaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCGCAGCAACCACAAAACCGCAGTTTTTATACGCCCGCGCCCGCAGCCCAGTCAGGGGATTGTAAGgcgccgcc > 4:237043/1‑145 (MQ=255) gaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCACTTAGCGGCCTTGCCAGCGAAGAGGCCGGCACACACACCACAAAACCGAAGTTTTTATCCGCCCCCTCACGCAACCCATTCAGGGTATTGAAAGGCGCATcca > 7:37394/1‑149 (MQ=255) tGCCCTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCCCAGCCACCACAAAACCTCAGTGTTTCTCCGCCCGCGCCCGCCCCCCGTTCAGGGGTATGACCGGCGCATCcaccacta > 8:246562/1‑147 (MQ=255) cccgagggCAGCC‑TTCTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGGGCAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTATCCGCCCGCTCACGCAGCCCTTTAAGGGTATTGAACGGCGCATCCCCCACGAAc > 3:43366/6‑149 (MQ=255) cccTCGGGCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAAc < 2:162942/149‑1 (MQ=255) gggcatgtttttttATTTTTCCGTGTGCTGCCCTTTGCGGGGCTTGGCCGGCTATGCGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACtt < 2:244794/140‑1 (MQ=255) gCAGCC‑TTTTTTATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTgg > 3:95010/1‑149 (MQ=255) ccttttttATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAt < 4:204038/113‑1 (MQ=255) ccttttttATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAt > 3:204038/3‑115 (MQ=255) ttttttATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGGACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccacca > 1:60140/1‑149 (MQ=255) ttttttATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTc > 2:8873/1‑93 (MQ=255) ttttttATTTGTCCT‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTc < 1:8873/93‑1 (MQ=255) | CCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTG‑GTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCA > NC_002947/4740682‑4740954 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |