Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 344,142 C→A 8.2% noncoding (34/76 nt) PP_t72 ← tRNA‑Phe

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947344,1420CA 8.2% 139.0 / 4.1 49noncoding (34/76 nt)PP_t72tRNA‑Phe
Reads supporting (aligned to +/- strand):  ref base C (23/22);  new base A (2/2);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

CGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGTCATTCGGCCGGTGGAACG  >  NC_002947/343997‑344284
                                                                                                                                                  |                                                                                                                                              
cGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCaa                                                                                                                                              <  1:9041/148‑1 (MQ=255)
cGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCa                                                                                                                                               <  6:71907/147‑1 (MQ=255)
      cgccTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTGAAAATCctcgt                                                                                                                                       <  8:62154/149‑3 (MQ=255)
      cgccTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTGAAAATCctc                                                                                                                                         >  5:184467/1‑147 (MQ=255)
      cgccTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTGAAAATCctc                                                                                                                                         <  6:184467/147‑1 (MQ=255)
           gAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTa                                                                                                                                  >  5:131050/1‑149 (MQ=255)
            aaGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTAc                                                                                                                                 <  2:238369/149‑1 (MQ=255)
            aaGTTAAAAATCACAGACAACAAAAAACACTCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTa                                                                                                                                  <  7:187877/148‑1 (MQ=255)
                 aaaaaTCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACt                                                                                                                            >  1:250893/1‑149 (MQ=255)
                             caacaaAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGTTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTggg                                                                                                                <  2:250893/149‑1 (MQ=255)
                             caacaaAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTggg                                                                                                                <  2:180528/149‑1 (MQ=255)
                              aacaaAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGc                                                                                                               >  8:220207/1‑149 (MQ=255)
                                caaAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTg                                                                                                                           <  1:231712/135‑1 (MQ=255)
                                caaAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTg                                                                                                                           >  2:231712/1‑135 (MQ=255)
                                 aagaaaCCCGC‑CTAGTGCGGGTTTTTTGTTGTCGCTTCGGGTAACTTTGCAACCACGAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGcgac                                                                                                            >  2:209311/4‑149 (MQ=255)
                                 aagaaaCCCGC‑CTAGTGCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACAACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTACAATCCTCTGATCTACCGACTGAGCTATCTGGGcgac                                                                                                            >  6:76130/4‑149 (MQ=255)
                                  aaaaaCCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGcgacg                                                                                                           <  2:36335/149‑1 (MQ=255)
                                        ccGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCTCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTg                                                                                                      >  5:12312/1‑148 (MQ=255)
                                        ccGCACTAG‑GCGCGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTg                                                                                                      >  7:208431/1‑148 (MQ=255)
                                              tAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTAt                                                                                                                     >  8:4115/1‑127 (MQ=255)
                                              tAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTAt                                                                                                                     <  7:4115/127‑1 (MQ=255)
                                                       tttCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTc                                                                                       <  4:189449/149‑1 (MQ=255)
                                                            tgttgtCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATACTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGAc                                                                                  >  7:189891/1‑149 (MQ=255)
                                                                    cTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAATTAGATTTCCGGACCGCTCGTc                                                                          <  6:131050/149‑1 (MQ=255)
                                                                        gggTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAAc                                                                       <  6:12312/148‑1 (MQ=255)
                                                                         ggTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGa                                                                     <  7:220207/149‑1 (MQ=255)
                                                                                ttGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTATACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACttttt                                                               >  4:162409/1‑148 (MQ=255)
                                                                                      ccaccaGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTTGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGaaaaaa                                                        >  8:102693/1‑149 (MQ=255)
                                                                                       caccaGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAAt                                                       >  6:190269/1‑149 (MQ=255)
                                                                                         ccaGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACttttt                                                               >  6:126246/1‑139 (MQ=255)
                                                                                         ccaGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACttttt                                                               <  5:126246/139‑1 (MQ=255)
                                                                                            gAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTa                                                   >  7:144189/1‑148 (MQ=255)
                                                                                             aaGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTTGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTaaa                                                 <  5:76130/149‑1 (MQ=255)
                                                                                                     aGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTcc                                         <  8:215216/149‑1 (MQ=255)
                                                                                                             cccAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTa                                 <  8:144189/149‑1 (MQ=255)
                                                                                                                     cGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGAttttt                         <  8:208431/149‑1 (MQ=255)
                                                                                                                         gTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTACGTCGCTTACGATTTTTgggg                     >  7:1096/1‑149 (MQ=255)
                                                                                                                            gAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCt                                   <  2:175538/132‑1 (MQ=255)
                                                                                                                            gAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCt                                   >  1:175538/1‑132 (MQ=255)
                                                                                                                            gAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGTCa                  >  1:194845/1‑149 (MQ=255)
                                                                                                                                       acGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGt                    <  6:53710/136‑1 (MQ=255)
                                                                                                                                       acGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGt                    >  5:53710/1‑136 (MQ=255)
                                                                                                                                        agAGGATTGAAAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCAGTCGCTTACGATTTTTTGGGTCATTCGGCCGGTg       >  1:197936/2‑148 (MQ=255)
                                                                                                                                         gAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGTCATTCGGCCGGTgg      >  3:201654/1‑148 (MQ=255)
                                                                                                                                          aGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGAGCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGTCATTCGGCCGGTGGaa    <  5:190269/149‑1 (MQ=255)
                                                                                                                                           ggATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAAt                                                       <  6:168808/97‑1 (MQ=255)
                                                                                                                                           ggATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAAt                                                       >  5:168808/1‑97 (MQ=255)
                                                                                                                                            gATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGTCATTCGGCCGGTGGAACg  >  3:117637/1‑149 (MQ=255)
                                                                                                                                            gATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGTCATTCGGCCGGTGGAACg  >  1:246247/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CGACGCCGCCTGAAGTTAAAAATCACAGACAACAAAAAACCCGCACTAG‑GCGGGTTTCTTGTTGTCGCTTCGGGTAACTTTGCAACCACCAGAAGCTTGAAGGTGGTGCCCAGAGACGGAGTCGAACCGCCGACACGAGGATTTTCAATCCTCTGCTCTACCGACTGAGCTATCTGGGCGACGAGGTGAATTAAATAGATTTCCGGACCGCTCGTCAACGACTTTTTGAAAAAATTTTAAATTAATTCCGTCGCTTACGATTTTTGGGGTCATTCGGCCGGTGGAACG  >  NC_002947/343997‑344284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑