Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,792,975 C→T 6.7% A289T (GCC→ACC)  pvdI ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,792,9750CT 6.7% 175.0 / 2.4 60A289T (GCC→ACC) pvdInon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (25/30);  new base T (2/2);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

AAGCAACCGATCAGCCCCTCCACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAGATGATCCAACCAGTACTCCA  >  NC_002947/4792827‑4793121
                                                                                                                                                    |                                                                                                                                                  
aaGCAACCGATCAGCCCCTCCACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGc                                                                                                                                                    >  1:176268/1‑149 (MQ=255)
   cAACCGATCAGCCCCTCCACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAg                                                                                                                                                 <  2:173524/149‑1 (MQ=255)
    aaCCGATCAGCCCCTCCACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAgg                                                                                                                                                <  4:24760/149‑1 (MQ=255)
       cGATCAGCCCCTCCACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGTCAGGGcc                                                                                                                                             >  7:18528/1‑149 (MQ=255)
         aTCAGCCCCTCCACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTg                                                                                                                                           >  5:37211/1‑149 (MQ=255)
               cccTCCACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGgcg                                                                                                                                     >  4:242226/1‑149 (MQ=255)
                 cTCCACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCt                                                                                                                                   >  4:161653/1‑149 (MQ=255)
                 cTCCACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCt                                                                                                                                   >  3:247645/1‑149 (MQ=255)
                    cACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTg                                                                                                                                  <  8:172834/147‑1 (MQ=255)
                        tCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGCGCCTGCAGGCCCTggccagg                                                                                                                            >  7:162013/1‑144 (MQ=255)
                          aCTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAgg                                                                                                                                       >  6:19095/1‑136 (MQ=255)
                          aCTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAgg                                                                                                                                       <  5:19095/136‑1 (MQ=255)
                          aCTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAAc                                                                                                                           <  5:137218/148‑1 (MQ=255)
                                gttgcggttgGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCAt                                                                                                                    <  6:142536/149‑1 (MQ=255)
                                gttgcggttgGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCAt                                                                                                                    <  7:95407/149‑1 (MQ=255)
                                       ttgGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTg                                                                                                             >  3:177704/1‑149 (MQ=255)
                                       ttgGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTg                                                                                                             >  3:181070/1‑149 (MQ=255)
                                             aTCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACCGCGTGACGTTGTGCCGCCTTGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTc                                                                                                       <  5:259968/149‑1 (MQ=255)
                                                ggCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGG‑CAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAg                                                                                                    <  6:103648/148‑1 (MQ=255)
                                                 gCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAgg                                                                                                   >  4:45594/1‑149 (MQ=255)
                                                     acCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGtt                                                                                               >  2:1482/1‑149 (MQ=255)
                                                       cGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCg                                                                                             >  1:159054/1‑149 (MQ=255)
                                                                  cgcgACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCt                                                                                  <  4:177704/149‑1 (MQ=255)
                                                                  cgcgACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCt                                                                                  <  4:181070/149‑1 (MQ=255)
                                                                   gcgACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTg                                                                                 >  8:252570/1‑149 (MQ=255)
                                                                   gcgACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTg                                                                                 <  3:242226/149‑1 (MQ=255)
                                                                    cgACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTgg                                                                                <  4:15953/149‑1 (MQ=255)
                                                                    cgACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTgg                                                                                <  1:1482/149‑1 (MQ=255)
                                                                     gACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGTCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTggg                                                                               <  8:18528/149‑1 (MQ=255)
                                                                            cGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGcc                                                                        <  4:164184/149‑1 (MQ=255)
                                                                                  aGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGc                                                                                                            <  8:16089/107‑1 (MQ=255)
                                                                                  aGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGc                                                                                                            >  7:16089/1‑107 (MQ=255)
                                                                                    cGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAg                                                                <  3:45594/149‑1 (MQ=255)
                                                                                      gTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGt                                                              >  8:22506/1‑149 (MQ=255)
                                                                                       tACATCAAGGGCGTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTg                                                             <  5:213219/149‑1 (MQ=255)
                                                                                       tACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTc                                                                                              <  6:18386/116‑1 (MQ=255)
                                                                                       tACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTc                                                                                              >  5:18386/1‑116 (MQ=255)
                                                                                          aacaaGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAg                                                                                    >  4:98947/1‑123 (MQ=255)
                                                                                          aacaaGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAg                                                                                    <  3:98947/123‑1 (MQ=255)
                                                                                          aacaaGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATc                                                          >  3:202929/1‑149 (MQ=255)
                                                                                          aacaaGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATc                                                          <  4:247645/149‑1 (MQ=255)
                                                                                               ggTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCg                                                     >  8:206700/1‑149 (MQ=255)
                                                                                                 tCTTGAACGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGc                                                   <  7:206700/149‑1 (MQ=255)
                                                                                                 tCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCgg                                                    <  8:208644/148‑1 (MQ=255)
                                                                                                 tCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCgg                                                    >  7:208644/1‑148 (MQ=255)
                                                                                                     gAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGTCAGGGCCTGCAGGCGCTggg                                                                                                                                >  8:228927/1‑68 (MQ=255)
                                                                                                     gAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGTCAGGGCCTGCAGGCGCTggg                                                                                                                                <  7:228927/68‑1 (MQ=255)
                                                                                                     gAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAAtt                                               >  6:122198/1‑149 (MQ=255)
                                                                                                      aaaGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAAtt                                               <  7:252570/148‑1 (MQ=255)
                                                                                                       aaGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGc                                             <  2:176268/149‑1 (MQ=255)
                                                                                                       aaGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGc                                             <  2:159054/149‑1 (MQ=255)
                                                                                                         gCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCaa                                           <  4:202929/149‑1 (MQ=255)
                                                                                                             ccAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACg                                       <  3:161653/149‑1 (MQ=255)
                                                                                                                      ccacGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCg                              >  6:135823/1‑149 (MQ=255)
                                                                                                                          gAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGc                          >  5:220411/1‑149 (MQ=255)
                                                                                                                          gAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGc                          >  3:96781/1‑149 (MQ=255)
                                                                                                                             cAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCa                       <  5:135823/149‑1 (MQ=255)
                                                                                                                                         gtgCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAgat                    <  4:13876/140‑1 (MQ=255)
                                                                                                                                         gtgCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAgat                    >  3:13876/1‑140 (MQ=255)
                                                                                                                                            ccgccTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAGATGATCCAACCAg         >  7:92888/1‑148 (MQ=255)
                                                                                                                                                   gCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAGATGATCCAACCAGTACTCCa  <  7:22506/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AAGCAACCGATCAGCCCCTCCACTTCACTGCGGTTGCGGTTGGCGATCGGCACACCGACGCGTACCCGCGACTGGCCGCTGTAGCGGTACAACAAGGTCTTGAAAGCGGCCAGCAGCACCACGAACAGCGTGACGTTGTGCCGCCTGGCCAGGGCCTGCAGGCGCTGGGCCAACGCTGCATCGATTTGCAGCTCCAGGCGTTCGCCACGGTAGCTGGGCAGCGCCGGACGCAGGTGATCGCTCGGCAATTGCAACACGGGGTGGTCGTTGCCCAGATGATCCAACCAGTACTCCA  >  NC_002947/4792827‑4793121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑