Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,796,617 G→C 22.2% intergenic (‑175/+43) pvdH ← / ← PP_4224 diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,796,6170GC22.2% 64.1 / 16.8 36intergenic (‑175/+43)pvdH/PP_4224diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (14/14);  new base C (4/4);  total (18/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.18e-01

TGGAAGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAAT  >  NC_002947/4796469‑4796764
                                                                                                                                                    |                                                                                                                                                    
tGGAAGCGGTGGACATGGGTAAATCCTCGCGAGCATGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAg                                                                                                                                                      >  8:117256/1‑149 (MQ=255)
tGGAAGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAg                                                                                                                                                      >  4:80684/1‑149 (MQ=255)
       ggTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACTCTTGAGTGGGGGGAGGATTTAGCGGGTGTTACGCGATTTTATTTTTGTCTCTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTAcgggcggg                                                                                                                                               <  6:277452/149‑1 (MQ=38)
       ggTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCggg                                                                                                                                               <  6:29314/149‑1 (MQ=255)
           gACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCa                                                                                                                                           <  1:75325/149‑1 (MQ=255)
              aTGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGCCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTA‑ccc                                                                                                                                       <  1:141777/149‑1 (MQ=37)
                 ggTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTTAACCCGCCCGTAGGAGCGGGTTCA‑CCcgc                                                                                                                                     >  1:248203/1‑148 (MQ=255)
                     aaTCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAaga                                                                                                                                <  8:51237/149‑1 (MQ=255)
                      aTCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTA‑CCCGCGAagag                                                                                                                               <  3:200030/149‑1 (MQ=37)
                      aTCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAagag                                                                                                                               <  7:23895/149‑1 (MQ=255)
                         cTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGc                                                                                                                             <  3:80684/148‑1 (MQ=255)
                            gcgAGCACGGGGTGGGGCGGTTATCGCTTTGTAACCGCTTGAGTGGGGGGAGTATTTAGGGGTTGTTACGCGATTTTTTTTTTGTCTGTGCTGGCCTCTTCGCGGTTAAACCCGCCCTTCGCAGCGGGTTCA‑CCCGCTAAGAGGCCa                                                                                                                           <  2:248203/147‑1 (MQ=255)
                                  aaGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGc                                                                                                                   <  3:38729/149‑1 (MQ=255)
                                               gTTATCGCTTTGCAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAACCCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAAt                                                                                                      <  2:257344/149‑1 (MQ=255)
                                                       tttGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGCCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTA‑CCcgc                                                                                                                                     <  6:180265/110‑1 (MQ=21)
                                                       tttGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGCCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTA‑CCcgc                                                                                                                                     >  5:180265/1‑110 (MQ=21)
                                                             aaCTCTTGAGTGGGGGTAGTATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACgcgc                                                                                         <  2:188772/148‑1 (MQ=255)
                                                                 cTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCGCGTAGGAGCGGGTTCG‑CCCGCGATGAGGGCGGCACGGGCGAGGTAGATCGTTGCCCTGGCCGCGCCggga                                                                                    >  4:192672/1‑147 (MQ=37)
                                                                           gggAGGATTTAGCGGTTGTTGCGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGcaaca                                                                           >  6:233367/1‑148 (MQ=255)
                                                                           gggAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGcaaca                                                                           >  6:83552/1‑148 (MQ=255)
                                                                            ggaggaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTTGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACaaa                                                                         <  1:85591/149‑1 (MQ=255)
                                                                                  tttAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCg                                                                    >  2:188349/1‑148 (MQ=255)
                                                                                         gttgttACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCAGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCg                                                             >  1:74776/1‑148 (MQ=255)
                                                                                               aCGCGATTTTATTTTTGTCTGTTCTGGCCTCTTAGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CACGCGAAGAGGCCAGAACAGGCGATAAACATCAATGCCCAGGACGCCCCGGTCCCTGCAACAAAACACGCAAGc                                                               >  8:183562/1‑140 (MQ=38)
                                                                                               aCGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGc                                                               <  7:183562/140‑1 (MQ=255)
                                                                                                      tttattttTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGTACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGt                                                >  7:85384/1‑148 (MQ=255)
                                                                                                                 cTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAGCCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGGCACTACGGCTGTCAAAGCGCAgg                                     >  1:59417/1‑149 (MQ=255)
                                                                                                                   gtgCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTAc                                                                                   <  5:226070/100‑1 (MQ=255)
                                                                                                                   gtgCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAACAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTAc                                                                                   >  6:226070/1‑100 (MQ=255)
                                                                                                                             ctTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCaaa                                            >  7:234201/1‑129 (MQ=255)
                                                                                                                             ctTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCaaa                                            <  8:234201/129‑1 (MQ=255)
                                                                                                                                    ggTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc                 >  1:112735/1‑149 (MQ=255)
                                                                                                                                     gTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc                 >  5:104290/1‑148 (MQ=255)
                                                                                                                                      tgaaCCCGCTCCTACGGGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGc                                    <  6:120502/126‑1 (MQ=37)
                                                                                                                                      tgaaCCCGCTCCTACGGGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGc                                    >  5:120502/3‑128 (MQ=37)
                                                                                                                                       gaaCCCGCTCCTACGGGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGAt              >  3:208513/2‑149 (MQ=38)
                                                                                                                                        aaCCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATc             <  2:74776/149‑1 (MQ=255)
                                                                                                                                          cccgctccTACGGGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAAAGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGc           >  5:97148/1‑149 (MQ=38)
                                                                                                                                              cccgTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGAGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTAACTCCGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTg        >  1:207096/1‑148 (MQ=255)
                                                                                                                                                    ggAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCATGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAAt  <  7:117256/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGAAGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAAT  >  NC_002947/4796469‑4796764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑