| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,796,627 | C→T | 19.9% | intergenic (‑185/+33) | pvdH ← / ← PP_4224 | diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,796,627 | 0 | C | T | 19.9% | 76.1 / 12.7 | 40 | intergenic (‑185/+33) | pvdH/PP_4224 | diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase |
| Reads supporting (aligned to +/- strand): ref base C (16/16); new base T (4/4); total (20/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.20e-01 | |||||||||||
GACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCC > NC_002947/4796480‑4796768 | gACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCa < 1:75325/149‑1 (MQ=255) aTGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGCCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTA‑ccc < 1:141777/149‑1 (MQ=37) ggTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTTAACCCGCCCGTAGGAGCGGGTTCA‑CCcgc > 1:248203/1‑148 (MQ=255) aaTCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAaga < 8:51237/149‑1 (MQ=255) aTCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTA‑CCCGCGAagag < 3:200030/149‑1 (MQ=37) aTCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAagag < 7:23895/149‑1 (MQ=255) cTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGc < 3:80684/148‑1 (MQ=255) gcgAGCACGGGGTGGGGCGGTTATCGCTTTGTAACCGCTTGAGTGGGGGGAGTATTTAGGGGTTGTTACGCGATTTTTTTTTTGTCTGTGCTGGCCTCTTCGCGGTTAAACCCGCCCTTCGCAGCGGGTTCA‑CCCGCTAAGAGGCCa < 2:248203/147‑1 (MQ=255) aaGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGc < 3:38729/149‑1 (MQ=255) gTTATCGCTTTGCAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAACCCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAAt < 2:257344/149‑1 (MQ=255) tttGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGCCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTA‑CCcgc < 6:180265/110‑1 (MQ=21) tttGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGCCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTA‑CCcgc > 5:180265/1‑110 (MQ=21) aaCTCTTGAGTGGGGGTAGTATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACgcgc < 2:188772/148‑1 (MQ=255) cTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCGCGTAGGAGCGGGTTCG‑CCCGCGATGAGGGCGGCACGGGCGAGGTAGATCGTTGCCCTGGCCGCGCCggga > 4:192672/1‑147 (MQ=37) gggAGGATTTAGCGGTTGTTGCGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGcaaca > 6:233367/1‑148 (MQ=255) gggAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGcaaca > 6:83552/1‑148 (MQ=255) ggaggaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTTGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACaaa < 1:85591/149‑1 (MQ=255) tttAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCg > 2:188349/1‑148 (MQ=255) gttgttACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCAGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCg > 1:74776/1‑148 (MQ=255) aCGCGATTTTATTTTTGTCTGTTCTGGCCTCTTAGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CACGCGAAGAGGCCAGAACAGGCGATAAACATCAATGCCCAGGACGCCCCGGTCCCTGCAACAAAACACGCAAGc > 8:183562/1‑140 (MQ=38) aCGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGc < 7:183562/140‑1 (MQ=255) tttattttTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGTACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGt > 7:85384/1‑148 (MQ=255) cTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAGCCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGGCACTACGGCTGTCAAAGCGCAgg > 1:59417/1‑149 (MQ=255) gtgCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTAc < 5:226070/100‑1 (MQ=255) gtgCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAACAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTAc > 6:226070/1‑100 (MQ=255) ctTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCaaa > 7:234201/1‑129 (MQ=255) ctTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCaaa < 8:234201/129‑1 (MQ=255) ggTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc > 1:112735/1‑149 (MQ=255) gTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc > 5:104290/1‑148 (MQ=255) tgaaCCCGCTCCTACGGGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGc < 6:120502/126‑1 (MQ=37) tgaaCCCGCTCCTACGGGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGc > 5:120502/3‑128 (MQ=37) gaaCCCGCTCCTACGGGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGAt > 3:208513/2‑149 (MQ=38) aaCCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATc < 2:74776/149‑1 (MQ=255) cccgctccTACGGGCGGGTTTA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAAAGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGc > 5:97148/1‑149 (MQ=38) cccgTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGAGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTAACTCCGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTg > 1:207096/1‑148 (MQ=255) ggAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCATGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAAt < 7:117256/148‑1 (MQ=255) gCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGcc < 5:236501/149‑1 (MQ=255) gCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGc > 6:128574/1‑148 (MQ=255) gTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTg > 2:138171/1‑135 (MQ=255) gTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTg < 1:138171/135‑1 (MQ=255) | GACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCA‑CCCGCGAAGAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGCC > NC_002947/4796480‑4796768 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |