Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,807,246 T→C 7.3% K216E (AAG→GAG)  PP_4234 ← bifunctional aldehyde oxidase/xanthine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,807,2460TC 7.3% 153.1 / 3.1 55K216E (AAG→GAG) PP_4234bifunctional aldehyde oxidase/xanthine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (27/23);  new base C (2/2);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01

AGCCAGTCCTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTGGCCTGG  >  NC_002947/4807101‑4807394
                                                                                                                                                   |                                                                                                                                                        
agccagTCCTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTCCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGAATGGAGTTCTTCAg                                                                                                                                                       >  5:164522/1‑149 (MQ=255)
 gccagTCCTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAgg                                                                                                                                                      >  4:198920/1‑149 (MQ=255)
  ccagTCCTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAGC‑AGCATGGACTTCTTCAGGc                                                                                                                                                     >  7:114089/1‑149 (MQ=255)
  ccagTCCTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGc                                                                                                                                                     >  8:1463/1‑149 (MQ=255)
   cagTCCTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCt                                                                                                                                                    >  7:20506/1‑149 (MQ=255)
      ggcgcgggcgggTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCGGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGga                                                                                                                                                 <  8:114089/145‑1 (MQ=255)
       ccTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGCGTTCTGCAGGCTg                                                                                                                                                   >  8:145037/1‑146 (MQ=255)
         tCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGaa                                                                                                                                              >  8:37386/1‑149 (MQ=255)
               gggTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCAtc                                                                                                                                        <  7:1463/149‑1 (MQ=255)
               gggTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTCCAGCCTGAAGAACTccat                                                                                                                                        <  6:226348/149‑4 (MQ=255)
               gggTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTCCAGCCTGAAGAACTccat                                                                                                                                        <  6:222680/149‑4 (MQ=255)
                 gTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCAGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAGATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCAtcg                                                                                                                                       <  1:192102/149‑1 (MQ=255)
                        aGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGaa                                                                                                                               <  7:8259/149‑1 (MQ=255)
                               cGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTg                                                                                                                        >  3:33445/1‑149 (MQ=255)
                                            ggCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCcacccacc                                                                                                            <  6:121835/148‑1 (MQ=255)
                                              cGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACt                                                                                                                                            >  8:173206/1‑114 (MQ=255)
                                              cGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACt                                                                                                                                            <  7:173206/114‑1 (MQ=255)
                                              cGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTg                                                                                                                        <  2:110531/134‑1 (MQ=255)
                                              cGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTg                                                                                                                        >  1:110531/1‑134 (MQ=255)
                                                tAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCAc                                                                                                       >  3:203504/1‑149 (MQ=255)
                                                    acaacCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAg                                                                                                   >  2:42119/1‑149 (MQ=255)
                                                       acCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑g                                                                                             >  5:117277/1‑148 (MQ=255)
                                                           cTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGttcttc                                                                                                                                                         >  2:172186/1‑88 (MQ=255)
                                                           cTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGc                                                                                        >  8:108487/1‑149 (MQ=255)
                                                              cTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGc                                                                                     <  1:163688/149‑1 (MQ=255)
                                                                  gTCAGGTCGCCGTGCTCGTTGGGCTGCTTCGACCACAACGGCTTGCGCTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCAt                                                                                  >  8:12795/1‑148 (MQ=255)
                                                                   tgaGGTAGTCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGATA‑ATGGTGGTGAAC‑ATCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTAGAACGGCGTGGCTCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCAt                                                                                  <  6:164522/145‑1 (MQ=255)
                                                                       gTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGcc                                                                                                                   <  8:148420/114‑1 (MQ=255)
                                                                       gTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGcc                                                                                                                   >  7:148420/1‑114 (MQ=255)
                                                                        tCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTc                                                                           >  8:155932/1‑149 (MQ=255)
                                                                                    ttGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAg                                                               <  8:20506/149‑1 (MQ=255)
                                                                                     tGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAgc                                                              >  2:81068/1‑149 (MQ=255)
                                                                                       ggCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAgcgg                                                            <  3:198920/149‑1 (MQ=255)
                                                                                         ctgctTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAACGAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAgcggc                                                           >  1:52010/1‑149 (MQ=255)
                                                                                            ctTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCCCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCt                                                       >  4:30939/1‑149 (MQ=255)
                                                                                             ttCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATAGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCAATAAGGGTCGCGCTGGTACAGCGGCGCCTg                                                      >  7:118233/1‑149 (MQ=255)
                                                                                               cGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTg                                                      <  6:117277/147‑1 (MQ=255)
                                                                                                gACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTggg                                                    <  3:30939/148‑1 (MQ=255)
                                                                                                   cacaCCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCAcc                                                >  4:204993/1‑149 (MQ=255)
                                                                                                    acacCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCg                                               >  8:130702/1‑149 (MQ=255)
                                                                                                     cacCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCg                                               >  3:240858/1‑148 (MQ=255)
                                                                                                        cGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAAc                                           <  4:33445/149‑1 (MQ=255)
                                                                                                        cGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAAATCATCGTCGTCGAACGGCTTGCCGCCACCCACCCCCACGAAGC‑‑‑‑GGAAGCTCCCATAAGGTACCCGCTGGTACAccggc                                                           >  6:63289/1‑133 (MQ=255)
                                                                                                         ggCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACg                                          <  4:240858/149‑1 (MQ=255)
                                                                                                               ctgTTGAGA‑ATGGTGGGGAAC‑TTCATGGAGTTCTTCAGGCTGGAGTACTAATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGTGTCGCGCTTGTACAGCGGCGACTgtg                                                    <  7:97904/131‑3 (MQ=255)
                                                                                                                   tGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCctgc                                >  3:169184/1‑149 (MQ=255)
                                                                                                                    gaga‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCctgc                                <  7:155932/148‑1 (MQ=255)
                                                                                                                      ga‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGa                             <  1:42119/149‑1 (MQ=255)
                                                                                                                         aTGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGAt                            <  7:37386/148‑1 (MQ=255)
                                                                                                                            gtggGGAAC‑AGCATGGAGTTCTTCAGGCTTGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGaa                        <  3:204993/149‑1 (MQ=255)
                                                                                                                            gtggGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGaa                        <  7:108487/149‑1 (MQ=255)
                                                                                                                              ggggTAC‑AGGATTCAGTTGTTCTGTCTTGAGAATTCATCTTCGTGGTACTGCGTGGTGCCACCCACCCCCCCGTACCACATGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGAtt                           <  1:219033/148‑1 (MQ=255)
                                                                                                                                      gacgatgAGTTCTCCAGCCTGAAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCtt               >  3:179873/7‑149 (MQ=255)
                                                                                                                                        cATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTgc             >  4:108240/1‑149 (MQ=255)
                                                                                                                                         gatgAGTTCTCCAGCCTGAAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTgcg            >  1:165437/4‑149 (MQ=255)
                                                                                                                                           cgatctcttcAGGGTGTAGAACTCATCGTAGTCTAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTgcgc           <  8:246611/143‑1 (MQ=255)
                                                                                                                                              gTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTgg       >  2:186123/1‑149 (MQ=255)
                                                                                                                                                 cttcAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTGGCCt    >  3:195860/1‑149 (MQ=255)
                                                                                                                                                  ttcAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTccgc           >  7:246611/1‑141 (MQ=255)
                                                                                                                                                   tcAGGCTGGAGAACTCATCGTCGTCGAACGGCGTCGCGCCAACCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAAATGCAGCTTGCGCATGGCCTgg  >  8:188305/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                        
AGCCAGTCCTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTGGCCTGG  >  NC_002947/4807101‑4807394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑