Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,807,250 G→C 8.7% S214R (AGC→AGG)  PP_4234 ← bifunctional aldehyde oxidase/xanthine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,807,2500GC 8.7% 166.2 / 7.3 58S214R (AGC→AGG) PP_4234bifunctional aldehyde oxidase/xanthine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (29/23);  new base C (2/3);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

GCCAGTCCTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTGGCCTGGC  >  NC_002947/4807102‑4807395
                                                                                                                                                      |                                                                                                                                                     
gccagTCCTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAgg                                                                                                                                                       >  4:198920/1‑149 (MQ=255)
 ccagTCCTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAGC‑AGCATGGACTTCTTCAGGc                                                                                                                                                      >  7:114089/1‑149 (MQ=255)
 ccagTCCTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGc                                                                                                                                                      >  8:1463/1‑149 (MQ=255)
  cagTCCTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCt                                                                                                                                                     >  7:20506/1‑149 (MQ=255)
     ggcgcgggcgggTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCGGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGga                                                                                                                                                  <  8:114089/145‑1 (MQ=255)
      ccTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGCGTTCTGCAGGCTg                                                                                                                                                    >  8:145037/1‑146 (MQ=255)
        tCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGaa                                                                                                                                               >  8:37386/1‑149 (MQ=255)
              gggTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCAtc                                                                                                                                         <  7:1463/149‑1 (MQ=255)
              gggTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTCCAGCCTGAAGAACTccat                                                                                                                                         <  6:226348/149‑4 (MQ=255)
              gggTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTCCAGCCTGAAGAACTccat                                                                                                                                         <  6:222680/149‑4 (MQ=255)
                gTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCAGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAGATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCAtcg                                                                                                                                        <  1:192102/149‑1 (MQ=255)
                       aGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGaa                                                                                                                                <  7:8259/149‑1 (MQ=255)
                              cGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTg                                                                                                                         >  3:33445/1‑149 (MQ=255)
                                           ggCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCcacccacc                                                                                                             <  6:121835/148‑1 (MQ=255)
                                             cGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACt                                                                                                                                             >  8:173206/1‑114 (MQ=255)
                                             cGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACt                                                                                                                                             <  7:173206/114‑1 (MQ=255)
                                             cGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTg                                                                                                                         >  1:110531/1‑134 (MQ=255)
                                             cGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTg                                                                                                                         <  2:110531/134‑1 (MQ=255)
                                               tAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCAc                                                                                                        >  3:203504/1‑149 (MQ=255)
                                                   acaacCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAg                                                                                                    >  2:42119/1‑149 (MQ=255)
                                                      acCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑g                                                                                              >  5:117277/1‑148 (MQ=255)
                                                          cTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGc                                                                                         >  8:108487/1‑149 (MQ=255)
                                                             cTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGc                                                                                      <  1:163688/149‑1 (MQ=255)
                                                                 gTCAGGTCGCCGTGCTCGTTGGGCTGCTTCGACCACAACGGCTTGCGCTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCAt                                                                                   >  8:12795/1‑148 (MQ=255)
                                                                  tgaGGTAGTCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGATA‑ATGGTGGTGAAC‑ATCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTAGAACGGCGTGGCTCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCAt                                                                                   <  6:164522/145‑1 (MQ=255)
                                                                      gTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGcc                                                                                                                    <  8:148420/114‑1 (MQ=255)
                                                                      gTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGcc                                                                                                                    >  7:148420/1‑114 (MQ=255)
                                                                       tCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTc                                                                            >  8:155932/1‑149 (MQ=255)
                                                                                   ttGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAg                                                                <  8:20506/149‑1 (MQ=255)
                                                                                    tGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAgc                                                               >  2:81068/1‑149 (MQ=255)
                                                                                      ggCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAgcgg                                                             <  3:198920/149‑1 (MQ=255)
                                                                                        ctgctTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAACGAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAgcggc                                                            >  1:52010/1‑149 (MQ=255)
                                                                                           ctTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCCCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCt                                                        >  4:30939/1‑149 (MQ=255)
                                                                                            ttCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATAGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCAATAAGGGTCGCGCTGGTACAGCGGCGCCTg                                                       >  7:118233/1‑149 (MQ=255)
                                                                                              cGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTg                                                       <  6:117277/147‑1 (MQ=255)
                                                                                               gACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTggg                                                     <  3:30939/148‑1 (MQ=255)
                                                                                                  cacaCCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCAcc                                                 >  4:204993/1‑149 (MQ=255)
                                                                                                   acacCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCg                                                >  8:130702/1‑149 (MQ=255)
                                                                                                    cacCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCg                                                >  3:240858/1‑148 (MQ=255)
                                                                                                       cGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAAc                                            <  4:33445/149‑1 (MQ=255)
                                                                                                       cGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAAATCATCGTCGTCGAACGGCTTGCCGCCACCCACCCCCACGAAGC‑‑‑‑GGAAGCTCCCATAAGGTACCCGCTGGTACAccggc                                                            >  6:63289/1‑133 (MQ=255)
                                                                                                        ggCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACg                                           <  4:240858/149‑1 (MQ=255)
                                                                                                              ctgTTGAGA‑ATGGTGGGGAAC‑TTCATGGAGTTCTTCAGGCTGGAGTACTAATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGTGTCGCGCTTGTACAGCGGCGACTgtg                                                     <  7:97904/131‑3 (MQ=255)
                                                                                                                  tGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCctgc                                 >  3:169184/1‑149 (MQ=255)
                                                                                                                   gaga‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCctgc                                 <  7:155932/148‑1 (MQ=255)
                                                                                                                     ga‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGa                              <  1:42119/149‑1 (MQ=255)
                                                                                                                        aTGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGAt                             <  7:37386/148‑1 (MQ=255)
                                                                                                                           gtggGGAAC‑AGCATGGAGTTCTTCAGGCTTGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGaa                         <  3:204993/149‑1 (MQ=255)
                                                                                                                           gtggGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGaa                         <  7:108487/149‑1 (MQ=255)
                                                                                                                             ggggTAC‑AGGATTCAGTTGTTCTGTCTTGAGAATTCATCTTCGTGGTACTGCGTGGTGCCACCCACCCCCCCGTACCACATGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGAtt                            <  1:219033/148‑1 (MQ=255)
                                                                                                                                     gacgatgAGTTCTCCAGCCTGAAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCtt                >  3:179873/7‑149 (MQ=255)
                                                                                                                                       cATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTgc              >  4:108240/1‑149 (MQ=255)
                                                                                                                                        gatgAGTTCTCCAGCCTGAAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTgcg             >  1:165437/4‑149 (MQ=255)
                                                                                                                                          cgatctcttcAGGGTGTAGAACTCATCGTAGTCTAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTgcgc            <  8:246611/143‑1 (MQ=255)
                                                                                                                                             gTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTgg        >  2:186123/1‑149 (MQ=255)
                                                                                                                                                cttcAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTGGCCt     >  3:195860/1‑149 (MQ=255)
                                                                                                                                                 ttcAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTccgc            >  7:246611/1‑141 (MQ=255)
                                                                                                                                                  tcAGGCTGGAGAACTCATCGTCGTCGAACGGCGTCGCGCCAACCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAAATGCAGCTTGCGCATGGCCTgg   >  8:188305/1‑149 (MQ=255)
                                                                                                                                                   cAGCCTGAAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTGGCCTGGc  <  2:165437/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCCAGTCCTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGA‑ATGGTGGGGAAC‑AGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGC‑‑‑‑GGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTGGCCTGGC  >  NC_002947/4807102‑4807395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑