Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 357,337 T→A 8.4% Y228N (TAC→AAC)  tdcG‑I → L‑serine dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947357,3370TA 8.4% 136.0 / 3.1 48Y228N (TAC→AAC) tdcG‑IL‑serine dehydratase
Reads supporting (aligned to +/- strand):  ref base T (24/19);  new base A (2/2);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

CTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAATGGGTCAACCTGTTCGCCTTGGCGGTCAAT  >  NC_002947/357190‑357483
                                                                                                                                                   |                                                                                                                                                  
ctgctgCGCCTTTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTa                                                                                                                                                   <  8:254046/149‑1 (MQ=255)
       gcCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCGCTGGGAAGCCATGCAGGAATGCGTCCACAACGGCCTCACGGACGAGGGc                                                                                                                                            >  4:49474/1‑149 (MQ=255)
            tgCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACg                                                                                                                                         <  4:234311/147‑1 (MQ=255)
             gcaagcaaAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTa                                                                                                                                      >  1:242280/1‑149 (MQ=255)
                      aCGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCggg                                                                                                                             <  5:256287/149‑1 (MQ=255)
                          ccTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAgg                                                                                                                                              <  5:25651/128‑1 (MQ=255)
                          ccTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAgg                                                                                                                                              >  6:25651/1‑128 (MQ=255)
                              aGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGt                                                                                                                     >  4:15666/1‑149 (MQ=255)
                                                     cAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTg                                                                                               >  7:158858/1‑148 (MQ=255)
                                                           gaAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCa                                                                                        <  2:263725/149‑1 (MQ=255)
                                                              ggTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCggg                                                                                                                             >  6:42000/1‑109 (MQ=255)
                                                              ggTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCggg                                                                                                                             <  5:42000/109‑1 (MQ=255)
                                                               gTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCt                                                                                    >  5:71318/1‑149 (MQ=255)
                                                                    gcgcAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGACTGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAg                                                                                >  5:155815/1‑148 (MQ=255)
                                                                    gcgcAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCa                                                                                 <  6:71318/147‑1 (MQ=255)
                                                                    gcgcAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAg                                                                                >  8:240857/1‑148 (MQ=255)
                                                                    gcgcAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAg                                                                                >  8:181083/1‑148 (MQ=255)
                                                                                  aCATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGccc                                                                 <  6:99692/149‑1 (MQ=255)
                                                                                    aTCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCCCCGCAGCCTGCAGGAAATCGGCAAGCCCaa                                                               >  2:205174/1‑149 (MQ=255)
                                                                                     tCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGAACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAAc                                                              >  5:267696/1‑149 (MQ=255)
                                                                                     tCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGAACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAAc                                                              >  5:264060/1‑149 (MQ=255)
                                                                                       cgcgCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACg                                                             <  7:240857/148‑1 (MQ=255)
                                                                                                     caAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCa                                              >  2:194333/1‑149 (MQ=255)
                                                                                                            tGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGc                                       >  5:118587/1‑149 (MQ=255)
                                                                                                              ggAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGc                                       <  1:194333/147‑1 (MQ=255)
                                                                                                              ggAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCa                                     >  8:136955/1‑149 (MQ=255)
                                                                                                              ggAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCa                                     >  6:174916/1‑149 (MQ=255)
                                                                                                              ggAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCa                                     >  7:96988/1‑149 (MQ=255)
                                                                                                               caatacATGCAGTAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCTGCGGGCACACAGTCCGCCGCCGTCCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGca                                      >  5:189084/6‑146 (MQ=38)
                                                                                                                  gCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATgg                                  >  3:136503/1‑148 (MQ=255)
                                                                                                                    cATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAAt                               >  3:108923/1‑149 (MQ=255)
                                                                                                                     aTGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGa                                           <  1:127421/136‑1 (MQ=255)
                                                                                                                     aTGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGa                                           >  2:127421/1‑136 (MQ=255)
                                                                                                                       gCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAATggg                            >  3:95218/1‑149 (MQ=255)
                                                                                                                        cAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCTGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAATGGGt                           <  1:205174/149‑1 (MQ=255)
                                                                                                                                  tCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAATGGGTCAACCTGTTc                 <  4:136503/149‑1 (MQ=255)
                                                                                                                                      caacGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAATGGGTCAACCTGtt                  >  3:128369/1‑144 (MQ=255)
                                                                                                                                      caacGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAATGGGTCAACCTGtt                  <  4:128369/144‑1 (MQ=255)
                                                                                                                                        acGGCCTCAAGAACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAATGGGTCAACCTGTTCGCCTTg           <  6:267696/149‑1 (MQ=255)
                                                                                                                                        aaggCCTCAAGAACTAGTGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAATGGGTCAACCTGTTCGCCTTg           <  6:264060/147‑1 (MQ=255)
                                                                                                                                          ggCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAATGGGTCAACCTGTTCGCCTTGGc         >  6:116735/1‑149 (MQ=255)
                                                                                                                                          ggCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAATGGGTCAACCTGTTCGCCTTGGc         <  8:96988/149‑1 (MQ=255)
                                                                                                                                            ccTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAATGGGTCAACCTGTTCGCCTTg           <  6:27059/145‑1 (MQ=255)
                                                                                                                                            ccTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAATGGGTCAACCTGTTCGCCTTg           >  5:27059/1‑145 (MQ=255)
                                                                                                                                              tCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAATGGGTCAAc                       >  2:30395/1‑131 (MQ=255)
                                                                                                                                              tCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAATGGGTCAAc                       <  1:30395/131‑1 (MQ=255)
                                                                                                                                                 aGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAATGGGTCAACCTGTTCGCCTTGGCGGTCAAt  <  3:15666/149‑1 (MQ=255)
                                                                                                                                                 aGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAATGGGTCAACCTGTTCGCCTTGGCGGTCAAt  <  8:158858/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTGCTGCGCCTGTGCAAGCAAAACGACCTCAGCGTGTCGCAATTGATGATGGCCAACGAGAAGGTCTGGCGCAGCGAGGAAGACATCCGCGCTGGCCTGCACAAGCTCTGGGAAGCCATGCAGGAATGCGTCAACAACGGCCTCAAGTACGAGGGCACGCTACCCGGCGGGCTGAACGTGCGCCGCCGTGCCGCCAAGCTGCACCGCAGCCTGCAGGAAATCGGCAAGCCCAACGTGATCGGCTCGACCATGAGTGCCATGGAATGGGTCAACCTGTTCGCCTTGGCGGTCAAT  >  NC_002947/357190‑357483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑