Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,819,491 G→A 7.8% A3900V (GCC→GTC)  pvdL ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,819,4910GA 7.8% 147.8 / 2.2 51A3900V (GCC→GTC) pvdLnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (19/28);  new base A (2/2);  total (21/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATCACCCCTTTCGGCAGGCCGGTCGAGCCTGAGGTGTAGATCACGTAGGCGAGGTT  >  NC_002947/4819347‑4819633
                                                                                                                                                   |                                                                                                                                              
tcCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCg                                                                                                                                              <  8:59501/147‑1 (MQ=255)
  cAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGt                                                                                                                                            <  6:171004/147‑1 (MQ=255)
  cAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCt                                                                                                                                          >  5:224489/1‑149 (MQ=255)
     cACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTggg                                                                                                                                       <  4:12747/149‑1 (MQ=255)
      aCGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTggg                                                                                                                                       >  1:262138/1‑148 (MQ=255)
                cGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTcc                                                                                                                            >  3:224824/1‑149 (MQ=255)
                  gTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTg                                                                                                                          >  4:44237/1‑149 (MQ=255)
                       cTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCg                                                                                                                      >  2:54019/1‑148 (MQ=255)
                          cACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCt                                                                                                                    <  6:224489/147‑1 (MQ=255)
                                cgcCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGcc                                                                                                             <  5:65488/148‑1 (MQ=255)
                                   cagcagGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGCATGAGAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAgcgc                                                                                                              >  1:219155/1‑147 (MQ=255)
                                     gcagGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTc                                                                                                                             >  4:59123/1‑127 (MQ=255)
                                        ggCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATc                                                                                                                                  <  7:185792/119‑1 (MQ=255)
                                        ggCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATc                                                                                                                                  >  8:185792/1‑119 (MQ=255)
                                        ggCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGGCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGc                                                                                                    <  5:173862/149‑1 (MQ=255)
                                          cccTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGGGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCAc                                                                                                  >  7:47797/1‑149 (MQ=255)
                                           ccTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCAcc                                                                                                 <  3:44237/149‑1 (MQ=255)
                                               cGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCtt                                                                                               <  4:224824/147‑1 (MQ=255)
                                                  gTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGc                                                                                                    >  1:213030/1‑139 (MQ=255)
                                                  gTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGc                                                                                                    <  2:213030/139‑1 (MQ=255)
                                                         gCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGTGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGgtt                                                                                   <  1:54019/149‑1 (MQ=255)
                                                              gACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGACGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGgttgtt                                                                                >  7:90651/1‑147 (MQ=255)
                                                              gACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCAAGATGTCGAAGCTTTGCGAGACGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGgttgtt                                                                                >  3:260589/1‑147 (MQ=255)
                                                                 aTTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCa                                                                           >  6:77247/1‑149 (MQ=255)
                                                                       cACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTc                                                                                                                             >  5:158917/1‑93 (MQ=255)
                                                                       cACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTc                                                                                                                             <  6:158917/93‑1 (MQ=255)
                                                                        aCGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCcgcg                                                                    >  1:213724/1‑149 (MQ=255)
                                                                              tctACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCAt                                                                          <  4:258708/137‑1 (MQ=255)
                                                                              tctACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCAt                                                                          >  3:258708/1‑137 (MQ=255)
                                                                               ctACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCg                                                             <  7:222639/149‑1 (MQ=255)
                                                                                tACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGCGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCgctgct                                                               <  1:203361/146‑1 (MQ=255)
                                                                                  ccTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCg                                                             <  2:262138/146‑1 (MQ=255)
                                                                                  ccTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGa                                                            <  5:77247/147‑1 (MQ=255)
                                                                                   cTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGAcc                                                          >  4:223469/1‑148 (MQ=255)
                                                                                        cgcCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATCAcc                                                    >  1:220384/1‑149 (MQ=255)
                                                                                                aGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATCACCCCTTTCgg                                            <  3:223469/149‑1 (MQ=255)
                                                                                                aGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATCACCCCTTTCgg                                            <  2:261674/149‑1 (MQ=255)
                                                                                                   ggc‑‑g‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATCACCCCTTTCGGCAg                                         <  2:213724/149‑1 (MQ=255)
                                                                                                           aGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATCACCCCTTTCGGCAGGCCgg                                    <  2:219155/149‑1 (MQ=255)
                                                                                                           aGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATCACCCCTTTCGGCAGGCCgg                                    <  6:242120/149‑1 (MQ=255)
                                                                                                                   aCTGCCAGACCTAGATGTCGAAGCTTTGCGAGACGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATCACCCCTTTCGGCAGGCCGGTCGAGCCt                            <  4:260589/149‑1 (MQ=255)
                                                                                                                   aCTGCCAGACCGAGATGTCGAAGCTTTGCGAGACGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATCACCCCTTTCGGCAGGCCGGTCGAGCCt                            <  8:90651/149‑1 (MQ=255)
                                                                                                                       ccAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATc                                                       <  6:149942/118‑1 (MQ=255)
                                                                                                                       ccAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATCACCCCTTTCGGCAGGCCGGTCGAGCCTGAgg                        >  5:6076/1‑149 (MQ=255)
                                                                                                                        cAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATCACCCCTTTCGGCAGGCCGGTCGAGCCTGAGgt                       <  3:178496/149‑1 (MQ=255)
                                                                                                                            ccGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATCACCCCTTTCGGCAGGCCGGTCGAGCCTGAGGTGTAg                   <  3:200495/149‑1 (MQ=255)
                                                                                                                               agctGTCGAACCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCGCGCTGATCGCCAGGTTAGGGACGTTGCTCTGCTGGTTGTTGAGCATGCCGGGCTGCTCGACCATCACCCCTTTCGGCTGGCGGGTCGAGCCTGAGGTGTAGATc                <  6:269978/146‑1 (MQ=37)
                                                                                                                                  tGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATCACCCCTTTCGGCAGGCCGGTCGAGCCTGAGGTGTAGATCACg             <  2:220384/149‑1 (MQ=255)
                                                                                                                                      gAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATCACCCCTTTCga                                            >  3:155038/1‑113 (MQ=255)
                                                                                                                                      gAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATCACCCCTTTCga                                            <  4:155038/114‑2 (MQ=255)
                                                                                                                                             tGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATCACCCCTTTCGGCAGGCCGGTCGAGCCTGAGGTGTAGATCACGTAGGCGAGGtt  >  7:54530/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
TCCAGCACGGTGATGCCGGTGGCCTGCACGTGCGCCAGCAGGCCCTGCGGGTCATGGGCGATGACATTAGGCACGATCTCTACCTTGGCGCCGAACAGCGGC‑‑G‑CAGCCAGGAACTGCCAGACCGAGATGTCGAAGCTTTGCGAGGCGGTCTGGGCAATCACGTCCTGCTCGCTGAGCGCCAGGTAAGGCACCTTGCTCAGCTGGTTGTTGAGCATGCCGCGCTGCTCGACCATCACCCCTTTCGGCAGGCCGGTCGAGCCTGAGGTGTAGATCACGTAGGCGAGGTT  >  NC_002947/4819347‑4819633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑