Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,826,272 C→G 9.8% V1640L (GTG→CTG)  pvdL ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,826,2720CG 9.8% 156.1 / 6.4 51V1640L (GTG→CTG) pvdLnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (23/23);  new base G (3/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

CTCGCTGGCTTCGAACAGGGCCTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTG  >  NC_002947/4826129‑4826416
                                                                                                                                               |                                                                                                                                                  
cTCGCTGGCTTCGAACAGGGCCTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTcgc                                                                                                                                               >  5:250498/1‑149 (MQ=255)
         ttCGAACAGGGCCTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATgg                                                                                                                                     >  4:250689/1‑149 (MQ=255)
                aGGGCCTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGAc                                                                                                                              >  6:254109/1‑149 (MQ=255)
                     cTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCtgtt                                                                                                                         >  8:91881/1‑149 (MQ=255)
                            ggCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCAGCGACCTGTTGTTGCAg                                                                                                                  >  4:100448/1‑149 (MQ=255)
                             gCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGAc                                                                                                                              >  6:247079/1‑136 (MQ=255)
                             gCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGAc                                                                                                                              <  5:247079/136‑1 (MQ=255)
                                gCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGAGCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCg                                                                                                              <  7:98190/149‑1 (MQ=255)
                                       aTCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCGGCTCGGTTCGTg                                                                                                       >  6:54277/1‑149 (MQ=255)
                                       aTCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTg                                                                                                       <  3:250689/149‑1 (MQ=255)
                                              gCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGa                                                                                                 >  2:126350/1‑148 (MQ=255)
                                              gCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGAc                                                                                                <  6:28819/149‑1 (MQ=255)
                                                   gCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGc                                                                                            <  3:25170/148‑1 (MQ=255)
                                                        tGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACgc                                                                                      <  2:212323/149‑1 (MQ=255)
                                                        tGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACgc                                                                                      <  5:254109/149‑1 (MQ=255)
                                                           gcgAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGttgttg                                                                                                                     <  3:222574/115‑1 (MQ=255)
                                                           gcgAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACgctg‑c                                                                                  >  4:4703/1‑149 (MQ=255)
                                                            cgAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACgctg‑ct                                                                                 <  5:191098/149‑1 (MQ=255)
                                                               aaCGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACAGGTCGCGGCCATCTGG‑CGCGACGtgttgaa                                                                                                                      >  1:119157/1‑108 (MQ=37)
                                                               aaCGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACAGGTCGCGGCCATCTGG‑CGCGACGtgttgaa                                                                                                                      <  2:119157/110‑3 (MQ=37)
                                                                    tCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACt                                                                         >  1:232374/1‑149 (MQ=255)
                                                                        ggTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACg                                                                                       >  4:133438/1‑132 (MQ=255)
                                                                        ggTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACg                                                                                       <  3:133438/132‑1 (MQ=255)
                                                                        ggTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTgggg                                                                     <  1:126350/149‑1 (MQ=255)
                                                                        ggTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTgggg                                                                     <  4:64812/149‑1 (MQ=255)
                                                                                gCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTGCCTGCCCCAGTGTggcagcagc                                                              <  7:211060/149‑5 (MQ=255)
                                                                                gCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGt                                                             <  6:250498/149‑1 (MQ=255)
                                                                                              ccGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGc                                                                                                     >  5:210383/1‑96 (MQ=255)
                                                                                              ccGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGc                                                                                                     <  6:210383/96‑1 (MQ=255)
                                                                                               cGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGAcc                                              <  2:232374/149‑1 (MQ=255)
                                                                                                  gggCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTg                                           <  3:15765/149‑1 (MQ=255)
                                                                                                    gCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGcc                                         >  7:131717/1‑149 (MQ=255)
                                                                                                            gtcgtcCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGCCCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCAGGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGc                                 >  7:111314/1‑149 (MQ=255)
                                                                                                            gtcgtcCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGc                                                                                            <  5:191506/91‑1 (MQ=255)
                                                                                                            gtcgtcCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGc                                                                                            >  6:191506/1‑91 (MQ=255)
                                                                                                               gtcCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGctg‑ctg                                                                                <  4:148883/99‑1 (MQ=255)
                                                                                                               gtcCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGctg‑ctg                                                                                >  3:148883/1‑99 (MQ=255)
                                                                                                                     cAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTg                                                       >  2:76149/1‑118 (MQ=255)
                                                                                                                          cAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCtgt                   >  7:147473/1‑149 (MQ=255)
                                                                                                                          cAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCtgt                   >  8:63836/1‑149 (MQ=255)
                                                                                                                          cAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTAACgc                                                                                      >  4:149499/1‑83 (MQ=255)
                                                                                                                           aaTGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCtgtg                  >  1:108247/1‑149 (MQ=255)
                                                                                                                             tGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGcc                <  3:100448/149‑1 (MQ=255)
                                                                                                                                   gCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCGGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAggg          <  5:54277/149‑1 (MQ=255)
                                                                                                                                     aGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCGAAGGGAt        <  7:63836/149‑1 (MQ=255)
                                                                                                                                       caacAACAGGTCGCGGCCATCTGG‑CGCGACGTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCCTCTGTGCCAAAGGGAt        >  5:255298/4‑147 (MQ=38)
                                                                                                                                        aacAACAGGTCGCGGCCATCTGG‑CGCGACGTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATg                           >  7:11027/3‑127 (MQ=37)
                                                                                                                                        aacAACAGGTCGCGGCCATCTGG‑CGCGACGTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATg                           <  8:11027/125‑1 (MQ=37)
                                                                                                                                          cAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCAt                       >  3:142866/1‑129 (MQ=255)
                                                                                                                                          cAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCAt                       <  4:142866/129‑1 (MQ=255)
                                                                                                                                           aaCACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTg  >  1:237381/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CTCGCTGGCTTCGAACAGGGCCTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTG  >  NC_002947/4826129‑4826416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑