Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,826,294 C→G 10.2% Q1632H (CAG→CAC)  pvdL ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,826,2940CG10.2% 147.8 / 6.5 49Q1632H (CAG→CAC) pvdLnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (20/24);  new base G (3/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

CTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAAT  >  NC_002947/4826150‑4826441
                                                                                                                                                 |                                                                                                                                                    
cTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCtgtt                                                                                                                                                  >  8:91881/1‑149 (MQ=255)
       ggCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCAGCGACCTGTTGTTGCAg                                                                                                                                           >  4:100448/1‑149 (MQ=255)
           gCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGAGCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCg                                                                                                                                       <  7:98190/149‑1 (MQ=255)
                  aTCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCGGCTCGGTTCGTg                                                                                                                                >  6:54277/1‑149 (MQ=255)
                  aTCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTg                                                                                                                                <  3:250689/149‑1 (MQ=255)
                         gCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGa                                                                                                                          >  2:126350/1‑148 (MQ=255)
                         gCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGAc                                                                                                                         <  6:28819/149‑1 (MQ=255)
                              gCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGc                                                                                                                     <  3:25170/148‑1 (MQ=255)
                                   tGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACgc                                                                                                               <  5:254109/149‑1 (MQ=255)
                                   tGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACgc                                                                                                               <  2:212323/149‑1 (MQ=255)
                                      gcgAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGttgttg                                                                                                                                              <  3:222574/115‑1 (MQ=255)
                                      gcgAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACgctg‑c                                                                                                           >  4:4703/1‑149 (MQ=255)
                                       cgAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACgctg‑ct                                                                                                          <  5:191098/149‑1 (MQ=255)
                                          aaCGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACAGGTCGCGGCCATCTGG‑CGCGACGtgttgaa                                                                                                                                               >  1:119157/1‑108 (MQ=37)
                                          aaCGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACAGGTCGCGGCCATCTGG‑CGCGACGtgttgaa                                                                                                                                               <  2:119157/110‑3 (MQ=37)
                                               tCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACt                                                                                                  >  1:232374/1‑149 (MQ=255)
                                                   ggTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACg                                                                                                                <  3:133438/132‑1 (MQ=255)
                                                   ggTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACg                                                                                                                >  4:133438/1‑132 (MQ=255)
                                                   ggTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTgggg                                                                                              <  4:64812/149‑1 (MQ=255)
                                                   ggTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTgggg                                                                                              <  1:126350/149‑1 (MQ=255)
                                                           gCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTGCCTGCCCCAGTGTggcagcagc                                                                                       <  7:211060/149‑5 (MQ=255)
                                                           gCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGt                                                                                      <  6:250498/149‑1 (MQ=255)
                                                                         ccGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGc                                                                                                                              <  6:210383/96‑1 (MQ=255)
                                                                         ccGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGc                                                                                                                              >  5:210383/1‑96 (MQ=255)
                                                                          cGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGAcc                                                                       <  2:232374/149‑1 (MQ=255)
                                                                             gggCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTg                                                                    <  3:15765/149‑1 (MQ=255)
                                                                               gCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGcc                                                                  >  7:131717/1‑149 (MQ=255)
                                                                                       gtcgtcCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGCCCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCAGGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGc                                                          >  7:111314/1‑149 (MQ=255)
                                                                                       gtcgtcCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGc                                                                                                                     <  5:191506/91‑1 (MQ=255)
                                                                                       gtcgtcCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGc                                                                                                                     >  6:191506/1‑91 (MQ=255)
                                                                                          gtcCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGctg‑ctg                                                                                                         <  4:148883/99‑1 (MQ=255)
                                                                                          gtcCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGctg‑ctg                                                                                                         >  3:148883/1‑99 (MQ=255)
                                                                                                cAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTg                                                                                >  2:76149/1‑118 (MQ=255)
                                                                                                     cAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCtgt                                            >  7:147473/1‑149 (MQ=255)
                                                                                                     cAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCtgt                                            >  8:63836/1‑149 (MQ=255)
                                                                                                     cAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTAACgc                                                                                                               >  4:149499/1‑83 (MQ=255)
                                                                                                      aaTGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCtgtg                                           >  1:108247/1‑149 (MQ=255)
                                                                                                        tGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGcc                                         <  3:100448/149‑1 (MQ=255)
                                                                                                              gCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCGGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAggg                                   <  5:54277/149‑1 (MQ=255)
                                                                                                                aGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCGAAGGGAt                                 <  7:63836/149‑1 (MQ=255)
                                                                                                                  caacAACAGGTCGCGGCCATCTGG‑CGCGACGTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCCTCTGTGCCAAAGGGAt                                 >  5:255298/4‑147 (MQ=38)
                                                                                                                   aacAACAGGTCGCGGCCATCTGG‑CGCGACGTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATg                                                    >  7:11027/3‑127 (MQ=37)
                                                                                                                   aacAACAGGTCGCGGCCATCTGG‑CGCGACGTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATg                                                    <  8:11027/125‑1 (MQ=37)
                                                                                                                     cAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCAt                                                <  4:142866/129‑1 (MQ=255)
                                                                                                                     cAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCAt                                                >  3:142866/1‑129 (MQ=255)
                                                                                                                      aaCACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTg                           >  1:237381/1‑149 (MQ=255)
                                                                                                                           gTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCt                                              <  7:167089/125‑1 (MQ=255)
                                                                                                                                 gcCAGATGGCCGCGACCTGTTGTTGCAGGTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCAc                  >  1:151510/1‑148 (MQ=255)
                                                                                                                                  ccAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCa                <  3:4703/149‑1 (MQ=255)
                                                                                                                                                ccTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAAt  >  7:149367/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CTTGAGCGGCAGCTCGACATCGCAGGCCTGGCGGGTGCGCGAAACGATCTGGGTAGCCAGCAACGAGTGACCACCGAGGGCGAAGAAGTCGTCCTGCAGGCCAATGCGCGGCAGATTCAACACGTCGC‑GCCAGATGGCCGCGACCTGTTGTTGCAGCTCGGTTCGTGGCTCGACATGCTCACGCTG‑CTGCCACACTGGGGCAGGCAGTGCCTTGCGGTCGACCTTGCCGCTGGGGCCCAATGGCATCTGTGCCAAAGGGATGAGTTGCGCCGGCACCATGTAGGCTGGCAAT  >  NC_002947/4826150‑4826441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑