Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,874,348 A→T 7.3% I62N (ATC→AAC)  PP_4283 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,874,3480AT 7.3% 165.3 / 2.9 55I62N (ATC→AAC) PP_4283GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (21/30);  new base T (2/2);  total (23/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

CGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATATGCGCGTAGACGATGTCGTCCTGGGTACCGCTGCGGCCGGCCTTGCC  >  NC_002947/4874200‑4874490
                                                                                                                                                    |                                                                                                                                              
cGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGga                                                                                                                                                <  3:258735/149‑1 (MQ=255)
cGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGga                                                                                                                                                >  8:176896/1‑149 (MQ=255)
 gCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGgat                                                                                                                                               <  7:207004/149‑1 (MQ=255)
       accgaggtGGCGCGTTAGACCATGCGCCGCGAGACGAACACCTGGCGCGCCTCTTCGACGGGCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCCGCACCACGCTTGCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGt                                                                                                                                         <  5:35413/142‑1 (MQ=255)
                 cgcgTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACg                                                                                                                               >  7:76437/1‑149 (MQ=255)
                        gACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCa                                                                                                                            >  2:47098/1‑145 (MQ=255)
                           cATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGAtct                                                                                                                     >  5:273853/1‑149 (MQ=255)
                            aTGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGAtctc                                                                                                                    <  8:76437/149‑1 (MQ=255)
                              gcgcCGCGAGAACAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGAtctc                                                                                                                    <  2:67479/147‑1 (MQ=255)
                                     gagaAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAgcgc                                                                                                           >  3:72519/1‑149 (MQ=255)
                                      agaagaACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCt                                                                                                          >  8:118546/1‑149 (MQ=255)
                                       gaagaaCACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCtt                                                                                                         <  2:1002/149‑1 (MQ=255)
                                              acCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCt                                                                                                  >  7:95272/1‑149 (MQ=255)
                                                     gcgcCTGTTCGACGGTCGGTCTGGCCACCAGCGCACCGCGGTTCGGTCGCAGCAGCACGTCGCTTTCGTGGGCCAGGCGAGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCATAGATCTCGCGGAGCGCTTCCTGGCTGAACTTg                                                                                           <  8:16310/149‑1 (MQ=255)
                                                        ccTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTg                                                                                        <  4:257209/149‑1 (MQ=255)
                                                         ctctTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCaa                                                                                                                           >  4:87855/1‑113 (MQ=255)
                                                         ctctTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCaa                                                                                                                           <  3:87855/113‑1 (MQ=255)
                                                         ctctTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGc                                                                                       >  6:204825/1‑149 (MQ=255)
                                                         ctctTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGc                                                                                       >  2:97991/1‑149 (MQ=255)
                                                            ttCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCACTc                                                                                                 >  5:267065/1‑136 (MQ=255)
                                                            ttCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCACTc                                                                                                 <  6:267065/136‑1 (MQ=255)
                                                              cGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGAACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGccc                                                                                     <  4:250324/146‑1 (MQ=255)
                                                                aCGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGc                                                                                <  7:176896/149‑1 (MQ=255)
                                                                       ggCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACgc                                                                         <  6:273853/149‑1 (MQ=255)
                                                                        gCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCA‑CACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGcc                                                                                                                 >  7:185172/1‑107 (MQ=255)
                                                                        gCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCA‑CACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGcc                                                                                                                 <  8:185172/107‑1 (MQ=255)
                                                                          tGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACgctgc                                                                      >  3:205743/1‑149 (MQ=255)
                                                                            gCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTc                                                                    <  2:224859/149‑1 (MQ=255)
                                                                              caccacCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTc                                                                    >  5:6758/1‑147 (MQ=255)
                                                                              caccacCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGa                                                                  <  1:97991/149‑1 (MQ=255)
                                                                               accaccGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAg                                                                 <  1:47098/149‑1 (MQ=255)
                                                                                ccaccGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAgg                                                                <  4:244969/149‑1 (MQ=255)
                                                                                ccaccGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAgg                                                                <  2:91649/149‑1 (MQ=255)
                                                                                   ccgcaccgcGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATc                                                             <  1:187048/149‑1 (MQ=255)
                                                                                   ccgcaccgcGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGACTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATc                                                             <  8:175646/149‑1 (MQ=255)
                                                                                                  gTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATATgc                                              <  4:72519/149‑1 (MQ=255)
                                                                                                     gcagcagcaCCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATATgcgc                                            <  4:205743/148‑1 (MQ=255)
                                                                                                     gcagcagaaCCACGCATTCGTGGCACACGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGAACTCGCACATCCCTTCCTAGCTAAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCATCCAAAAAATCcgcg                                           >  4:30738/1‑149 (MQ=255)
                                                                                                              ccagGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATATGCGCGTAGACGATg                                  <  4:178773/149‑1 (MQ=255)
                                                                                                              ccacGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCTCCCAGCGCTTCCTCGCTCAACTTg                                                                                           <  2:133490/92‑1 (MQ=255)
                                                                                                              ccacGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTg                                                                                           >  1:133490/1‑92 (MQ=255)
                                                                                                               cacGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGTTGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATATGCGCGTAGACGATgt                                 >  5:174738/1‑149 (MQ=255)
                                                                                                               cacGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGTTGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATATGCGCGTAGACGATgt                                 >  5:170830/1‑149 (MQ=255)
                                                                                                               cacGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATATGCGCGTAGACGATgt                                 >  2:221115/1‑149 (MQ=255)
                                                                                                                  gCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATc                                                             <  4:164809/118‑1 (MQ=255)
                                                                                                                  gCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATc                                                             >  3:164809/1‑118 (MQ=255)
                                                                                                                             ccAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTg                                                                                           >  6:28352/1‑77 (MQ=255)
                                                                                                                             ccAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTg                                                                                           <  5:28352/77‑1 (MQ=255)
                                                                                                                             ccAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATATGCGCGTAGACGATGTCGTCCTGGGTACCg                   >  8:42269/1‑149 (MQ=255)
                                                                                                                                      aCAAGGCGCGGCGGTTGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATATGCGCGTAGACGATGTCGTCCTGGGTACCGCTGcggccg          <  6:170830/149‑1 (MQ=255)
                                                                                                                                      aCAAGGCGCGGCGGTTGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGATCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATATGCGCGTAGACGATGTCGTCCTGGGTACCGCTGcggccg          <  6:174738/149‑1 (MQ=255)
                                                                                                                                       cAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATATGCGCGTAGACGATgtcgtc                             >  4:19718/1‑129 (MQ=255)
                                                                                                                                       cAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATATGCGCGTAGACGATgtcgtc                             <  3:19718/129‑1 (MQ=255)
                                                                                                                                             gcggcgGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATATGCGCGTAGACGATGTCGTCCTGGGTACCGCTGcggccg          <  7:76291/142‑1 (MQ=255)
                                                                                                                                             gcggcgGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATATGCGCGTAGACGATGTCGTCCTGGGTACCGCTGcggccg          >  8:76291/1‑142 (MQ=255)
                                                                                                                                             gcggcgGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATATGCGCGTAGACGATGTCGTCCTGGGTACCGCTGCGGCCGGCCTTGc   <  7:118546/149‑1 (MQ=255)
                                                                                                                                              cggcggATGATGGTGCGGCTGACGCCAAAGCTCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATCTGCGCGTAGACGATGTCGTCCTGGGTACCGCTGCGGCCGGCCTTGcc  <  3:30738/149‑1 (MQ=255)
                                                                                                                                              cggcggATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATATGCGCGTAGACGATGTCGTCCTGGGTACCGCTGCGGCCGGCCTTGcc  <  8:159014/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CGCCAGCTCGGTGATGGCGCGTTCGACCATGCGCCGCGAGAAGAACACCTGGCGCGCCTCTTCGACGGTCGGGCTGGCCACCACCGCACCGCGGTTCGGTCGCAGCAGCACCACGCTTTCGTGGGCCAGGCGCGACAAGGCGCGGCGGATGATGGTGCGGCTGACGCCAAAGATCTCGCCCAGCGCTTCCTCGCTCAACTTGGTGCCCGGAGCCAGACGCTGCTCGAGGATCGCCTCGAAAATATGCGCGTAGACGATGTCGTCCTGGGTACCGCTGCGGCCGGCCTTGCC  >  NC_002947/4874200‑4874490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑