Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,900,051	G→A	9.1%	intergenic (+12/‑486)	PP_4308 → / → PP_4309	AsnC family transcriptional regulator/NCS1 family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,900,051	0	G	A	9.1%	120.0 / 2.5	44	intergenic (+12/‑486)	PP_4308/PP_4309	AsnC family transcriptional regulator/NCS1 family transporter
Reads supporting (aligned to +/- strand):  ref base G (17/22);  new base A (2/2);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

CTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTC  >  NC_002947/4899904‑4900195                                                                                                                                                    |                                                                                                                                                 cTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGACAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGc                                                                                                                                                 >  1:50750/1‑149 (MQ=255)    cgtcgCCCGACTGCATGTGCGCTCTATGGAGCAGCTGGACACCCTGCTCGACCTCTTGAATGTGCTGGCCGAGACCAATTCCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTg                                                                                                                                               <  2:114548/148‑1 (MQ=255)     gtcgCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGc                                                                                                                                             <  1:8128/149‑1 (MQ=255)                        gcTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCcg                                                                                                                          >  1:46996/1‑149 (MQ=255)                          tCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCcgc                                                                                                                         >  7:78241/1‑148 (MQ=255)                              tGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTACTGGCCTCTTCGCGGGCACGCCTGCGAaga                                                                                                                    <  1:235214/149‑1 (MQ=39)                                gAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCGGGCACGCCTGCGAagag                                                                                                                   <  1:154370/148‑1 (MQ=255)                                gAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCGGGCACGCCTGCGAagag                                                                                                                   <  5:11280/148‑1 (MQ=255)                                    agcTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGa                                                                                                                       <  2:148693/140‑1 (MQ=255)                                    agcTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGa                                                                                                                       >  1:148693/1‑140 (MQ=255)                                            acCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTACTGGCCTCTTCGCGGGCACGCCTGCGAagag                                                                                                                   <  4:233428/136‑1 (MQ=39)                                            acCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTACTGGCCTCTTCGCGGGCACGCCTGCGAagag                                                                                                                   >  3:233428/1‑136 (MQ=39)                                              ccTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTTCCCGCGAAGAGGCCAGTACAGGaaa                                                                                                     >  8:86416/1‑148 (MQ=255)                                               cTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCTTCTTCGCAGGCGTGCCCGCGAAGAGGCCAg                                                                                                              <  1:25038/138‑1 (MQ=255)                                               cTGCTAGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGAACATCAATAAGACCCCGGTCAAGCGGCGGTTGCCCCCAATGGATTGATTGGCTACTGTTCTGGCCTATTAGCACGCGTGCCCGGGAACAGGCCAGTatgtccg                                                                                                      >  2:25038/1‑140 (MQ=255)                                                     gACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAg                                                                                              <  2:50750/148‑1 (MQ=255)                                                     gACCTCATGAATGGGCTGGCCTAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTAGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGa                                                                                               <  8:160044/147‑1 (MQ=255)                                                           aTGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCTGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTAttttt                                                                                       <  8:134035/149‑1 (MQ=255)                                                           aTGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTAttttt                                                                                       >  8:93063/1‑149 (MQ=255)                                                           aTGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACACCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTACGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGATGAGTAttttt                                                                                       >  4:191836/1‑149 (MQ=255)                                                            tgtaTGTGCTGGCCTTGAGCAATACCGCGATCATCAAGAAGACCCCGGTCAATCGGCGGTTGCCGCCAATGGTTTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGATAAGCTGAGTAtttttt                                                                                      <  3:191836/146‑1 (MQ=255)                                                               aTGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTa                                                                                   >  2:175330/1‑149 (MQ=255)                                                                             aCCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTAtttttt                                                                                      >  4:217229/1‑132 (MQ=255)                                                                             aCCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTAtttttt                                                                                      <  3:217229/132‑1 (MQ=255)                                                                                        gATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGc                                                                                                                                             <  6:31401/66‑1 (MQ=255)                                                                                        gATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGc                                                                                                                                             >  5:31401/1‑66 (MQ=255)                                                                                             tcaAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCAttttt                                                     >  8:32735/1‑149 (MQ=255)                                                                                                    gaCCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCa                                              <  1:175330/149‑1 (MQ=255)                                                                                                           gTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTttctt                                       <  3:112031/149‑1 (MQ=255)                                                                                                           gTCAAGCGGCGGTTGCCGCCAACGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTttct                                        >  3:186239/1‑148 (MQ=255)                                                                                                              aaGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAg                                    >  8:171668/1‑149 (MQ=255)                                                                                                                gcggcgGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGc                                                                                                                                 >  7:92763/1‑54 (MQ=255)                                                                                                                gcggcgGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGc                                                                                                                                 <  8:92763/54‑1 (MQ=255)                                                                                                                   gcggTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGgcg                               <  7:32735/149‑1 (MQ=255)                                                                                                                       ttGCCGCCAATGGATTGATTGGCTCCGGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGcccc                           <  2:134335/149‑1 (MQ=255)                                                                                                                          ccgccAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTCTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAAt                        <  4:243480/149‑1 (MQ=255)                                                                                                                             ccAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTAAACAAAAACGTAACAATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAAttgg                     >  7:167594/1‑147 (MQ=255)                                                                                                                              cAATGGACTGATTGGCTCCTGTGCTGGCCTCATCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGt                    <  7:194426/149‑1 (MQ=255)                                                                                                                                aTGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTa                  <  2:81577/149‑1 (MQ=255)                                                                                                                                 tGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTAAACAAAAAGGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGAGACCCAATTCGTTa                  >  5:126910/1‑148 (MQ=255)                                                                                                                                   gattgattGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTaca                <  7:86416/148‑1 (MQ=255)                                                                                                                                             cTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCAcc     <  8:78241/149‑1 (MQ=255)                                                                                                                                              tCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCAGTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACCCACTTTCCAccc    >  7:214030/1‑149 (MQ=255)                                                                                                                                                cTGTACTGGCCTCTTCGCGGGCACGCCTGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCctc  >  1:234177/1‑149 (MQ=38)                                                                                                                                                    gCTGGCCTCTTAGCAGGCGTGCCCGCGAAGATGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGtt                   <  4:60923/128‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTC  >  NC_002947/4899904‑4900195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑