Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,900,070	T→C	15.5%	intergenic (+31/‑467)	PP_4308 → / → PP_4309	AsnC family transcriptional regulator/NCS1 family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,900,070	0	T	C	15.5%	97.3 / 11.9	45	intergenic (+31/‑467)	PP_4308/PP_4309	AsnC family transcriptional regulator/NCS1 family transporter
Reads supporting (aligned to +/- strand):  ref base T (20/18);  new base C (3/4);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-02

GCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGACCAAATGATCAAC  >  NC_002947/4899927‑4900216                                                                                                                                                |                                                                                                                                                   gcTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCcg                                                                                                                                               >  1:46996/1‑149 (MQ=255)   tCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCcgc                                                                                                                                              >  7:78241/1‑148 (MQ=255)       tGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTACTGGCCTCTTCGCGGGCACGCCTGCGAaga                                                                                                                                         <  1:235214/149‑1 (MQ=39)         gAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCGGGCACGCCTGCGAagag                                                                                                                                        <  1:154370/148‑1 (MQ=255)         gAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCGGGCACGCCTGCGAagag                                                                                                                                        <  5:11280/148‑1 (MQ=255)             agcTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGa                                                                                                                                            >  1:148693/1‑140 (MQ=255)             agcTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGa                                                                                                                                            <  2:148693/140‑1 (MQ=255)                     acCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTACTGGCCTCTTCGCGGGCACGCCTGCGAagag                                                                                                                                        >  3:233428/1‑136 (MQ=39)                     acCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTACTGGCCTCTTCGCGGGCACGCCTGCGAagag                                                                                                                                        <  4:233428/136‑1 (MQ=39)                       ccTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTTCCCGCGAAGAGGCCAGTACAGGaaa                                                                                                                          >  8:86416/1‑148 (MQ=255)                        cTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCTTCTTCGCAGGCGTGCCCGCGAAGAGGCCAg                                                                                                                                   <  1:25038/138‑1 (MQ=255)                        cTGCTAGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGAACATCAATAAGACCCCGGTCAAGCGGCGGTTGCCCCCAATGGATTGATTGGCTACTGTTCTGGCCTATTAGCACGCGTGCCCGGGAACAGGCCAGTatgtccg                                                                                                                           >  2:25038/1‑140 (MQ=255)                              gACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAg                                                                                                                   <  2:50750/148‑1 (MQ=255)                              gACCTCATGAATGGGCTGGCCTAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTAGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGa                                                                                                                    <  8:160044/147‑1 (MQ=255)                                    aTGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCTGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTAttttt                                                                                                            <  8:134035/149‑1 (MQ=255)                                    aTGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTAttttt                                                                                                            >  8:93063/1‑149 (MQ=255)                                    aTGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACACCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTACGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGATGAGTAttttt                                                                                                            >  4:191836/1‑149 (MQ=255)                                     tgtaTGTGCTGGCCTTGAGCAATACCGCGATCATCAAGAAGACCCCGGTCAATCGGCGGTTGCCGCCAATGGTTTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGATAAGCTGAGTAtttttt                                                                                                           <  3:191836/146‑1 (MQ=255)                                        aTGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTa                                                                                                        >  2:175330/1‑149 (MQ=255)                                                      aCCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTAtttttt                                                                                                           >  4:217229/1‑132 (MQ=255)                                                      aCCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTAtttttt                                                                                                           <  3:217229/132‑1 (MQ=255)                                                                      tcaAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCAttttt                                                                          >  8:32735/1‑149 (MQ=255)                                                                             gaCCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCa                                                                   <  1:175330/149‑1 (MQ=255)                                                                                    gTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTttctt                                                            <  3:112031/149‑1 (MQ=255)                                                                                    gTCAAGCGGCGGTTGCCGCCAACGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTttct                                                             >  3:186239/1‑148 (MQ=255)                                                                                       aaGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAg                                                         >  8:171668/1‑149 (MQ=255)                                                                                            gcggTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGgcg                                                    <  7:32735/149‑1 (MQ=255)                                                                                                ttGCCGCCAATGGATTGATTGGCTCCGGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGcccc                                                <  2:134335/149‑1 (MQ=255)                                                                                                   ccgccAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTCTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAAt                                             <  4:243480/149‑1 (MQ=255)                                                                                                      ccAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTAAACAAAAACGTAACAATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAAttgg                                          >  7:167594/1‑147 (MQ=255)                                                                                                       cAATGGACTGATTGGCTCCTGTGCTGGCCTCATCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGt                                         <  7:194426/149‑1 (MQ=255)                                                                                                         aTGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTa                                       <  2:81577/149‑1 (MQ=255)                                                                                                          tGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTAAACAAAAAGGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGAGACCCAATTCGTTa                                       >  5:126910/1‑148 (MQ=255)                                                                                                            gattgattGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTaca                                     <  7:86416/148‑1 (MQ=255)                                                                                                                      cTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCAcc                          <  8:78241/149‑1 (MQ=255)                                                                                                                       tCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCAGTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACCCACTTTCCAccc                         >  7:214030/1‑149 (MQ=255)                                                                                                                         cTGTACTGGCCTCTTCGCGGGCACGCCTGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCctc                       >  1:234177/1‑149 (MQ=38)                                                                                                                             gCTGGCCTCTTAGCAGGCGTGCCCGCGAAGATGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGtt                                        <  4:60923/128‑1 (MQ=255)                                                                                                                                 gCCTCTTCGCGGGCACGCCTGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGa               >  1:131492/1‑149 (MQ=255)                                                                                                                                 gCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTACCACACTCTATTATGa               >  4:18633/1‑149 (MQ=255)                                                                                                                                  ccTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGa               >  1:125515/1‑148 (MQ=255)                                                                                                                                    tctTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGgcg                                                    >  6:203379/1‑109 (MQ=255)                                                                                                                                    tctTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGgcg                                                    <  5:203379/109‑1 (MQ=255)                                                                                                                                      ttCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGACCaaa          >  4:225911/1‑149 (MQ=255)                                                                                                                                               gTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGACCAAATGATCAAc  >  5:8983/1‑148 (MQ=255)                                                                                                                                                |                                                                                                                                                   GCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCCTCTTCGCAGGCGTGCCCGCGAAGAGGCCAGTACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACGTAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCTATTTTGACCAAATGATCAAC  >  NC_002947/4899927‑4900216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑