Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,915,752	A→T	12.1%	V112V (GTT→GTA)	ccmC ←	protoheme IX reservoir complex subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,915,752	0	A	T	12.1%	80.2 / 2.1	31	V112V (GTT→GTA)	ccmC	protoheme IX reservoir complex subunit
Reads supporting (aligned to +/- strand):  ref base A (17/10);  new base T (2/2);  total (19/12)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGCACTATCACGATTACTGATCGCCTGGCCCAGCGCAATAATGCCGAAGTACAGGAACAGCAGG‑ATGAGCATGGACGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGTTTCATCTTCCACACCAGCCCCACCACCCCGGCCACCGCCAGCAGCACATAGCAGGACTGCGCCAGCATCGCCGCCGGCA  >  NC_002947/4915612‑4915897                                                                                                                                              |                                                                                                                                                  gCCGCACTATCACGATTACTGATCGCCTGGCCCAGCGCAATAATGCCGAAGTACAGGAACAGCAGGTATGAGCATGGACGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAgcg                                                                                                                                            >  1:80882/1‑149 (MQ=255)   cGCACTATCACGATTACTGATCGCCTGGCCCAGCGCAATAAAGCCGAACTACAGGAACAGCAGG‑ATGAGCATGGACGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGAAAACCAGCGCCa                                                                                                                                         >  1:92169/1‑149 (MQ=255)          tCACTATTACTGATCGCCTGGCCCAGCGCAATAATGCCGAAGTACAGGAACAGCAGG‑ATGAGCATGGACGTAATGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCAcggcgg                                                                                                                                   >  5:212986/1‑148 (MQ=255)            aCGATTACTGATCGCCTGGCCCAGCGCAATAATGCCGAAGTACAGGAACAGCAGG‑ATGAGCATGGACGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCa                                                                                                                                >  5:253930/1‑149 (MQ=255)                   cTGATCGCCTGGCCCAGCGCAATAATGCCGAAGTACAGGAACAGCAGG‑ATGAGCATGGACGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGgc                                                                                                                         <  6:75433/149‑1 (MQ=255)                      aTCGCCTGGCCCAGCGCAATAATGCCGAAGTACAGGAACAGCAGG‑ATGAGCATGGACGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGgcgc                                                                                                                       >  6:41032/1‑148 (MQ=255)                         gCCTGGCCCAGCGGAATTATGCCGTAGTTCAGGAACAGCAGG‑ATGAGCATGGAGGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCTGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCAGGGCGGTCATCCAGGCGCCaa                                                                                                                    <  2:92169/148‑1 (MQ=255)                                       aataatGCCGAAGTACAGGAACAGCAGG‑ATGAGCATGGACGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACt                                                                                                     <  5:41032/149‑1 (MQ=255)                                           atGCCGCAGTACAGGAACAGCAGG‑ATGAGCATGGACGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAg                                                                                                 <  7:168901/149‑1 (MQ=255)                                                     aCAGGAACAGCAGG‑ATGAGCATGGACGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGTCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCg                                                                                       >  4:100322/1‑149 (MQ=255)                                                        ggAACAGCAGG‑ATGAGCATGGACGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTg                                                                                                    <  3:167332/133‑1 (MQ=255)                                                        ggAACAGCAGG‑ATGAGCATGGACGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTg                                                                                                    >  4:167332/1‑133 (MQ=255)                                                         gAACAGCAGG‑ATGAGCATGGACGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGc                                                                                     >  6:21848/1‑147 (MQ=255)                                                            cagcagG‑ATGAGCATGGACGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGtt                                                                                <  2:80882/149‑1 (MQ=255)                                                                     tGAGCATGGACGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGTTTCATCTTc                                                                        >  6:121161/1‑149 (MQ=255)                                                                               cGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCTGGTTACCGGCGCCatc                                                                                                                                       >  5:25612/1‑74 (MQ=38)                                                                               cGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCTGGTTACCGGCGCCatc                                                                                                                                       <  6:25612/76‑3 (MQ=38)                                                                                          cATCCCACACCCACCAGCTTCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCAGGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGTTTCATCTTCCACACCAGCCCCACCACCCCg                                                   <  6:212986/149‑1 (MQ=255)                                                                                          cATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGTTTCATCTTCCACACCAGCCCCACCACCCCg                                                   >  8:75812/1‑149 (MQ=255)                                                                                          cATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGTTTCATCTTCCACACCAGCCCCACCACCCCg                                                   <  5:257561/149‑1 (MQ=255)                                                                                                     caccaGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGTTTCATCTTCCACACCAGCCCCACCACCCCGGCCACCGCcag                                        >  8:26374/1‑149 (MQ=255)                                                                                                        caGCTGCCCCAGGTCGGCTTGCCCCAGAAGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGTTTCATCTTCCACACCAGCCCCACCACCCCGGCCACCGCcagcag                                     >  1:151941/1‑149 (MQ=255)                                                                                                               cccAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGTTTCATCTTCCACACCAGCCCCACCACCCCGGCCACCGCCAGCAGCACATAg                              >  3:245078/1‑149 (MQ=255)                                                                                                                ccAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGTTTCATCTTCCACACCAGCCCCACCACCCCGGCCACCGCCAGCAGCACATAg                              >  3:201356/1‑148 (MQ=255)                                                                                                                ccAGGTCGACTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGTTTCATCTTCCACACCAGCCCCACCACCCCGGCCACCGCCAGCAGCACATAg                              >  3:51330/1‑148 (MQ=255)                                                                                                                            gCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGTTTCATCTTCCACACCAGCCCCACCACCCCGGCCACCGCCAGCAGCACATAGCAGGACTgcgc                   <  7:26374/147‑1 (MQ=255)                                                                                                                             ccccAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGTTTCATCTTCCACACCAGCCCCACCACCCCGGCCACCGCCAGCAGCACATAGCAGGACTGCGCCAg                >  7:172831/1‑149 (MQ=255)                                                                                                                              cccAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGGCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGTTTGATCTTCCACACCAGCCCCACCACCCCGGCCACAGCCAGCAGCACAAAGCAGGACTGCGCCAg                >  1:215403/1‑148 (MQ=255)                                                                                                                                           ggTTACCGGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGTTTCATCTTCCACACCAGCCCCACCACCCCGGCCACCGCCAGCAGCACATAGCAGGACTGCGcc                  >  7:36483/1‑133 (MQ=255)                                                                                                                                           ggTTACCGGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGTTTCATCTTCCACACCAGCCCCACCACCCCGGCCACCGCCAGCAGCACATAGCAGGACTGCGcc                  <  8:36483/133‑1 (MQ=255)                                                                                                                                           ggTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGTTTCATCTTCCACACCAGCCCCACCACCCCGGCCACCGCCAGCAGCACATAGCAGGACTGCGCCAGCATCGCCGCCGGca  <  4:201356/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                  GCCGCACTATCACGATTACTGATCGCCTGGCCCAGCGCAATAATGCCGAAGTACAGGAACAGCAGG‑ATGAGCATGGACGTAAGGCGGGCATCCCACACCCACCAGCTGCCCCAGGTCGGCTTGCCCCAGATGGCGCCGGTAACCAGCGCCACGGCGGTCATCCAGGCGCCAATGGGCGCGGCACACTGCAGGGCGACGTCGGCCAGTTTCATCTTCCACACCAGCCCCACCACCCCGGCCACCGCCAGCAGCACATAGCAGGACTGCGCCAGCATCGCCGCCGGCA  >  NC_002947/4915612‑4915897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑