| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,949,944 | C→G | 6.9% | D134H (GAT→CAT) | fliM ← | flagellar biosynthesis switching/energizing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,949,944 | 0 | C | G | 6.9% | 151.2 / 3.5 | 58 | D134H (GAT→CAT) | fliM | flagellar biosynthesis switching/energizing protein |
| Reads supporting (aligned to +/- strand): ref base C (21/32); new base G (2/2); total (24/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-02 | |||||||||||
CGGAGTTCATGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTATTCGCCGAACTTCATG > NC_002947/4949800‑4950086 | cGGAGTTCATGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATcgccg > 1:2124/1‑149 (MQ=255) aTGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGa > 4:102403/1‑148 (MQ=255) tGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTAGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGaa > 2:237265/1‑148 (MQ=255) tACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAt‑t < 3:140304/149‑1 (MQ=255) cTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGt < 6:147547/149‑1 (MQ=255) cTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGt > 3:197581/1‑149 (MQ=255) tCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTc > 8:84395/1‑149 (MQ=255) tCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCATTCGATCTTGGCGTGGCGGCCATCGCCGACAAAGAAAT‑AGTc > 2:216337/1‑149 (MQ=255) gAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATAGCCGCCAAAGAAAT‑TTTcc > 7:129034/1‑148 (MQ=255) aGCTCACCGTAATGATCGGCTGCCTGGCTTCCTTGAGGTCGTCGAAACTCTGGTCCAGCACCATGCGCACCACAGGGATTTCTGTCGGGGTGAACTCACGCCGTTCGATCTTGGCGTGTCGGCCATCGCCGCCAAATAAAT‑TTTccac < 8:199771/148‑1 (MQ=255) tGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGc > 7:83394/1‑149 (MQ=255) tGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAacac > 3:206196/1‑148 (MQ=255) tCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTccac < 1:118780/133‑1 (MQ=255) ccTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTg > 2:207304/1‑149 (MQ=255) ggCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGa < 1:167742/149‑1 (MQ=255) gCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAg < 2:249010/149‑1 (MQ=255) ttGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGc < 7:50643/120‑1 (MQ=255) ttGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGc > 8:50643/1‑120 (MQ=255) gAGGTCGACGAACCTCTGGTCCAGGACGATGAGCACCAGACGCATTTCGGTCGTGGTGAACTCAGGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAAc < 7:49285/148‑1 (MQ=255) gAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAAc < 4:246545/148‑1 (MQ=255) gacgaAACCCTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGGGCACTCACGCCCTGCGCTCTGGGCGTGGCGGCCCTCGCCCCCAAAGGAAT‑AGTCCCCCACCTGGAACACCAGCGAGGCGTACAGGAGGCCCAGCCAAg > 7:118484/1‑149 (MQ=255) gacgaAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAg > 1:69572/1‑149 (MQ=255) cgaAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGa < 6:61859/132‑1 (MQ=255) cgaAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGa > 5:61859/1‑132 (MQ=255) acacTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCcgc < 1:237265/149‑1 (MQ=255) acTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCGTGGCGTCGAGGAGGACCAGCGAAGTGCcgcg > 2:196564/1‑148 (MQ=255) tGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAg > 7:58748/1‑149 (MQ=255) gTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATg < 5:83816/127‑1 (MQ=255) gTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATg > 6:83816/1‑127 (MQ=255) cAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCt < 8:83394/149‑1 (MQ=255) aCCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGAt < 1:196564/149‑1 (MQ=255) cccttcgcAACACACGCAGTTCTGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATc < 6:188133/144‑1 (MQ=255) ccATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATc < 7:84395/149‑1 (MQ=255) ccATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATc < 8:129034/149‑1 (MQ=255) cATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAAc < 4:90581/121‑1 (MQ=255) tGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGc < 6:116046/122‑1 (MQ=255) tGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGc > 5:116046/1‑122 (MQ=255) cgcACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCTCCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGAc > 7:225633/1‑149 (MQ=255) caccacACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCa < 4:197581/149‑1 (MQ=255) ggtcggGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCtggtg < 3:102403/148‑1 (MQ=255) tcggGGTTAACTCACGCCCTTCGATATTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCCGCTTGAACACCAGCTTGTCGTCGAGGATGAACAGCGAAGTGCCTAGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGc < 1:216337/149‑1 (MQ=255) ggggTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCAc < 5:25991/149‑1 (MQ=255) gTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCTCCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTa < 8:225633/149‑1 (MQ=255) aCGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGa < 8:58748/147‑1 (MQ=255) aCGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAg < 2:19639/148‑1 (MQ=255) ccTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCAc < 2:2124/149‑1 (MQ=255) ttCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCAc > 6:246663/1‑147 (MQ=255) ttCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCAc < 5:246663/147‑1 (MQ=255) ttCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACg > 7:204213/1‑148 (MQ=255) ttCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTAGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACg > 8:149111/1‑148 (MQ=255) tctttggcgGCGATGGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGa > 5:183083/5‑132 (MQ=255) tctttggcgGCGATGGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGa < 6:183083/128‑1 (MQ=255) ggcgtggcgGCCATCGCCGCCAAAGAAATGTGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCtggtgg > 1:20719/1‑120 (MQ=255) ggcgtggcgGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGGGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTTTTCGc < 1:235287/146‑1 (MQ=255) ggcgtggcgGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCtggtgg < 2:20719/119‑1 (MQ=255) ggcgtggcgGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTATTc < 8:204213/144‑1 (MQ=255) tttggcgGCGATGGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGc < 2:139282/132‑1 (MQ=255) tttggcgGCGATGGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGc > 1:139282/3‑134 (MQ=255) ggcCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTATTCGCCGAACTTCATg < 1:207304/149‑1 (MQ=255) | CGGAGTTCATGTACTCGAAGCTGACCGGCATGATCGCCTGCCAGGCTTCCTTGAGGTCGACGAAACACTGGTCCAGCACCATGCGCACCACACGCAGTTCGGTCGGGGTGAACTCACGCCCTTCGATCTTGGCGTGGCGGCCATCGCCGCCAAAGAAAT‑TGTCCACCAGCTTGAACACCAGCTTGGCGTCGAGGATGAACAGCGAAGTGCCGCGCAGCGGCTTGATCTTGACCAGGTTGAGGCTGGTGGGCACGTACAGCGAGTGCACGTATTCGCCGAACTTCATG > NC_002947/4949800‑4950086 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |