Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,963,027 G→C 6.9% L210V (CTG→GTG)  fleS ← two‑component system sensor histidine kinase FleS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,963,0270GC 6.9% 182.7 / 4.0 58L210V (CTG→GTG) fleStwo‑component system sensor histidine kinase FleS
Reads supporting (aligned to +/- strand):  ref base G (25/29);  new base C (2/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

GGGTCACCCGGTCCCCCAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAGGCGACGGGTTTCAGTCAGGTC  >  NC_002947/4962880‑4963162
                                                                                                                                                   |                                                                                                                                       
gggTCACCCGGTCCCCCAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAgg                                                                                                                                        >  7:42337/1‑149 (MQ=255)
 ggTCACCCGGTCCCCCAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTCGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGt                                                                                                                                       >  8:59198/1‑149 (MQ=255)
      cccGGTCCCCCAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCg                                                                                                                                  >  5:31535/1‑149 (MQ=255)
        cGGTCCCCCAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCgg                                                                                                                                 <  3:135089/148‑1 (MQ=255)
        cGGTCCCCCAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCgg                                                                                                                                 >  4:135089/1‑148 (MQ=255)
         ggTCCCCCAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCa                                                                                                                               <  3:16997/149‑1 (MQ=255)
         ggTCCCCCAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCa                                                                                                                               <  3:212631/149‑1 (MQ=255)
         ggTCCCCCAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCa                                                                                                                               <  4:256718/149‑1 (MQ=255)
              cccAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCg                                                                                                                                  >  5:3061/1‑141 (MQ=255)
              cccAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCg                                                                                                                                  <  6:3061/141‑1 (MQ=255)
              cccAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAg                                                                                                                          >  6:141827/1‑149 (MQ=255)
                cAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCa                                                                                                                        <  5:214711/149‑1 (MQ=255)
                 aGTGGCAACTCGCCACGGGCAAACACCAGCATCTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCGCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCAt                                                                                                                       <  6:259209/149‑1 (MQ=255)
                  gTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATg                                                                                                                      <  3:260186/149‑1 (MQ=255)
                  gTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATg                                                                                                                      <  6:31535/149‑1 (MQ=255)
                     gCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGCCGGCCACCTGGCCGACaacga                                                                                                                           <  5:261144/141‑5 (MQ=255)
                     gCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGCCGGCCACCTGGCCGACaacga                                                                                                                           >  6:261144/1‑137 (MQ=255)
                       aaCTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAg                                                                                                                                         <  6:59373/125‑1 (MQ=255)
                       aaCTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAg                                                                                                                                         >  5:59373/1‑125 (MQ=255)
                       aaCTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGc                                                                                                                 <  7:112965/149‑1 (MQ=255)
                             ccACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGTTGcctcgct                                                                                                           >  3:227619/1‑144 (MQ=255)
                                     aaaCACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGc                                                                                                   <  8:42337/149‑1 (MQ=255)
                                       acacCCGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAg                                                                                                                          <  7:190203/124‑1 (MQ=255)
                                       acacCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCATCAAGGTCGCGGCGAAGAGCTGACGGGTTTGCGCGCTCAGCGCTTGATCGTTGTCGGCAAGTTGGCCGGCATACat                                                                                                                          >  8:190203/1‑123 (MQ=255)
                                              cATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCtggt                                                                                          >  7:115466/1‑149 (MQ=255)
                                              cATGGCGCGCACCTGGTGTTCCAGTGCGTGCAGGCGCTCCTTCATGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCtggt                                                                                          <  7:254390/149‑1 (MQ=255)
                                                  tCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGGATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGc                                                                                      <  6:201489/149‑1 (MQ=255)
                                                  tCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGc                                                                                      >  2:28213/1‑149 (MQ=255)
                                                            ggTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGc                                                                             >  1:95137/1‑148 (MQ=255)
                                                             gtgtTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCaa                                                                           >  6:245218/1‑149 (MQ=255)
                                                             gtgtTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCaa                                                                           >  6:241157/1‑149 (MQ=255)
                                                                ttCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCa                                                                        >  7:221277/1‑149 (MQ=255)
                                                                   cAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATgc                                                                     >  6:150538/1‑149 (MQ=255)
                                                                     gTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATgcgc                                                                   <  2:95137/149‑1 (MQ=255)
                                                                            cAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAac                                                             <  8:221277/148‑1 (MQ=255)
                                                                                 gcTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAAcgacga                                                        >  5:168771/1‑148 (MQ=255)
                                                                                 gcTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAAcgacga                                                        >  7:201637/1‑148 (MQ=255)
                                                                                  cTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACa                                                      <  5:150538/149‑1 (MQ=255)
                                                                                     cTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCATCGAGGCAACCATGCGCCCCAACGACGACAGc                                                    <  1:28213/148‑1 (MQ=255)
                                                                                         aaGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTc                                               <  8:201637/149‑1 (MQ=255)
                                                                                         aaGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTc                                               >  7:157089/1‑149 (MQ=255)
                                                                                                 ggcgAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAg                                       >  6:265539/1‑149 (MQ=255)
                                                                                                          cTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTc                                               >  6:108826/1‑132 (MQ=255)
                                                                                                          cTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTc                                               <  5:108826/132‑1 (MQ=255)
                                                                                                             aCGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTc                             <  5:241157/147‑1 (MQ=255)
                                                                                                             aCGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTc                             <  5:245218/147‑1 (MQ=255)
                                                                                                               gggTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAAcgacga                                                        >  2:159632/1‑118 (MQ=255)
                                                                                                               gggTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAAcgacga                                                        <  1:159632/118‑1 (MQ=255)
                                                                                                                ggTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTg                          >  4:234292/1‑147 (MQ=255)
                                                                                                                ggTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGa                        <  5:141827/149‑1 (MQ=255)
                                                                                                                  tttGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAgg                      <  8:115466/149‑1 (MQ=255)
                                                                                                                      cgcgcTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAGGCGa                   <  6:168771/148‑1 (MQ=255)
                                                                                                                             aGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAGGCGACGGGttt            >  5:111710/1‑148 (MQ=255)
                                                                                                                                 cTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAGGCGACGGGTTtcagtc       <  4:148324/149‑1 (MQ=255)
                                                                                                                                      tCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAGGCGACGGGTTTCAGTCAGGTc  >  4:108637/1‑149 (MQ=255)
                                                                                                                                        tatgccGGCCACCTGGCCGACATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTg                          >  1:176146/6‑123 (MQ=255)
                                                                                                                                        tatgccGGCCACCTGGCCGACATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTg                          <  2:176146/118‑1 (MQ=255)
                                                                                                                                        gTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCtggt                                                                                          <  6:50524/59‑1 (MQ=255)
                                                                                                                                        gTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCtggt                                                                                          >  5:50524/1‑59 (MQ=255)
                                                                                                                                        gTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAGGCGACGGGTTtcag         >  1:261956/1‑140 (MQ=255)
                                                                                                                                        gTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAGGCGACGGGTTtcag         <  2:261956/140‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                       
GGGTCACCCGGTCCCCCAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAGGCGACGGGTTTCAGTCAGGTC  >  NC_002947/4962880‑4963162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑