Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,963,035 G→A 7.5% A207V (GCC→GTC)  fleS ← two‑component system sensor histidine kinase FleS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,963,0350GA 7.5% 150.6 / 2.8 53A207V (GCC→GTC) fleStwo‑component system sensor histidine kinase FleS
Reads supporting (aligned to +/- strand):  ref base G (22/27);  new base A (2/2);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

CGGTCCCCCAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAGGCGACGGGTTTCAGTCAGGTCGTTGAGCAGCACCAATTGG  >  NC_002947/4962888‑4963181
                                                                                                                                                   |                                                                                                                                                  
cGGTCCCCCAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCgg                                                                                                                                                    >  4:135089/1‑148 (MQ=255)
cGGTCCCCCAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCgg                                                                                                                                                    <  3:135089/148‑1 (MQ=255)
 ggTCCCCCAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCa                                                                                                                                                  <  4:256718/149‑1 (MQ=255)
 ggTCCCCCAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCa                                                                                                                                                  <  3:16997/149‑1 (MQ=255)
 ggTCCCCCAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCa                                                                                                                                                  <  3:212631/149‑1 (MQ=255)
      cccAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAg                                                                                                                                             >  6:141827/1‑149 (MQ=255)
        cAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCa                                                                                                                                           <  5:214711/149‑1 (MQ=255)
         aGTGGCAACTCGCCACGGGCAAACACCAGCATCTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCGCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCAt                                                                                                                                          <  6:259209/149‑1 (MQ=255)
          gTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATg                                                                                                                                         <  6:31535/149‑1 (MQ=255)
          gTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATg                                                                                                                                         <  3:260186/149‑1 (MQ=255)
             gCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGCCGGCCACCTGGCCGACaacga                                                                                                                                              >  6:261144/1‑137 (MQ=255)
             gCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGCCGGCCACCTGGCCGACaacga                                                                                                                                              <  5:261144/141‑5 (MQ=255)
               aaCTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGc                                                                                                                                    <  7:112965/149‑1 (MQ=255)
                     ccACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGTTGcctcgct                                                                                                                              >  3:227619/1‑144 (MQ=255)
                             aaaCACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGc                                                                                                                      <  8:42337/149‑1 (MQ=255)
                               acacCCGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAg                                                                                                                                             <  7:190203/124‑1 (MQ=255)
                               acacCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCATCAAGGTCGCGGCGAAGAGCTGACGGGTTTGCGCGCTCAGCGCTTGATCGTTGTCGGCAAGTTGGCCGGCATACat                                                                                                                                             >  8:190203/1‑123 (MQ=255)
                                      cATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCtggt                                                                                                             >  7:115466/1‑149 (MQ=255)
                                      cATGGCGCGCACCTGGTGTTCCAGTGCGTGCAGGCGCTCCTTCATGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCtggt                                                                                                             <  7:254390/149‑1 (MQ=255)
                                          tCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGGATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGc                                                                                                         <  6:201489/149‑1 (MQ=255)
                                          tCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGc                                                                                                         >  2:28213/1‑149 (MQ=255)
                                                    ggTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGc                                                                                                >  1:95137/1‑148 (MQ=255)
                                                     gtgtTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCaa                                                                                              >  6:245218/1‑149 (MQ=255)
                                                     gtgtTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCaa                                                                                              >  6:241157/1‑149 (MQ=255)
                                                        ttCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCa                                                                                           >  7:221277/1‑149 (MQ=255)
                                                           cAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATgc                                                                                        >  6:150538/1‑149 (MQ=255)
                                                             gTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATgcgc                                                                                      <  2:95137/149‑1 (MQ=255)
                                                                    cAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAac                                                                                <  8:221277/148‑1 (MQ=255)
                                                                         gcTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAAcgacga                                                                           >  5:168771/1‑148 (MQ=255)
                                                                         gcTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAAcgacga                                                                           >  7:201637/1‑148 (MQ=255)
                                                                          cTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACa                                                                         <  5:150538/149‑1 (MQ=255)
                                                                             cTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCATCGAGGCAACCATGCGCCCCAACGACGACAGc                                                                       <  1:28213/148‑1 (MQ=255)
                                                                                 aaGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTc                                                                  >  7:157089/1‑149 (MQ=255)
                                                                                 aaGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTc                                                                  <  8:201637/149‑1 (MQ=255)
                                                                                         ggcgAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAg                                                          >  6:265539/1‑149 (MQ=255)
                                                                                                  cTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTc                                                                  <  5:108826/132‑1 (MQ=255)
                                                                                                  cTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTc                                                                  >  6:108826/1‑132 (MQ=255)
                                                                                                     aCGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTc                                                <  5:245218/147‑1 (MQ=255)
                                                                                                     aCGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTc                                                <  5:241157/147‑1 (MQ=255)
                                                                                                       gggTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAAcgacga                                                                           >  2:159632/1‑118 (MQ=255)
                                                                                                       gggTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAAcgacga                                                                           <  1:159632/118‑1 (MQ=255)
                                                                                                        ggTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTg                                             >  4:234292/1‑147 (MQ=255)
                                                                                                        ggTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGa                                           <  5:141827/149‑1 (MQ=255)
                                                                                                          tttGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAgg                                         <  8:115466/149‑1 (MQ=255)
                                                                                                              cgcgcTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAGGCGa                                      <  6:168771/148‑1 (MQ=255)
                                                                                                                     aGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAGGCGACGGGttt                               >  5:111710/1‑148 (MQ=255)
                                                                                                                         cTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAGGCGACGGGTTtcagtc                          <  4:148324/149‑1 (MQ=255)
                                                                                                                              tCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAGGCGACGGGTTTCAGTCAGGTc                     >  4:108637/1‑149 (MQ=255)
                                                                                                                                tatgccGGCCACCTGGCCGACATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTg                                             >  1:176146/6‑123 (MQ=255)
                                                                                                                                tatgccGGCCACCTGGCCGACATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTg                                             <  2:176146/118‑1 (MQ=255)
                                                                                                                                gTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCtggt                                                                                                             <  6:50524/59‑1 (MQ=255)
                                                                                                                                gTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCtggt                                                                                                             >  5:50524/1‑59 (MQ=255)
                                                                                                                                gTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAGGCGACGGGTTtcag                            <  2:261956/140‑1 (MQ=255)
                                                                                                                                gTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAGGCGACGGGTTtcag                            >  1:261956/1‑140 (MQ=255)
                                                                                                                                                 cGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAGGCGACGGGTTTCAGTCAGGTCGTTGAGCAGCACCAATTgg  >  5:3338/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGGTCCCCCAGTGGCAACTCGCCACGGGCAAACACCAGCATGTCGCGCACCTGGTGTTCCAGTTCGTGCAGGCGCTCCTTCAAGGTCGCGGCGAAGCGCTGACGGGTTTGCGCGCTCAGCGCTTGCTCGTTGTCGGCCAGGTGGCCGGCATACAGCATGGCTGCCGACAGCGGCGTGCGGATCTGGTGGGCCAGCGAGGCAACCATGCGCCCCAACGACGACAGCCGCTCGTGGCGAGCCAGCTGGTCCTGGAGGCGACGGGTTTCAGTCAGGTCGTTGAGCAGCACCAATTGG  >  NC_002947/4962888‑4963181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑