| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,965,196 | G→T | 54.5% | C54* (TGC→TGA) | fleQ ← | transcriptional regulator FleQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,965,196 | 0 | G | T | 54.5% | 2.2 / 42.7 | 33 | C54* (TGC→TGA) | fleQ | transcriptional regulator FleQ |
| Reads supporting (aligned to +/- strand): ref base G (7/8); new base T (9/9); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
AAAGCACCCGGCGGCGAAGGTCTTCCGGCAGCTCGGCGGAAGAAATTTCACCTAAAAGCAAAACCGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTG > NC_002947/4965051‑4965343 | aaaGCACCCGGCGGCGAAGGTCTTCCGGCAGCTCGGCGGAAGAAATTTCACCTAAAAGCAAAACCGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAgcactcac < 1:55813/149‑1 (MQ=255) aaGCACCCGGCGGCGAAGGTCTTCCGGCAGCTCGGCTGAAGAAATTTCACCTAAAAGCAAAACCGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGcaca < 3:262788/149‑1 (MQ=255) gcggcgAAGGTCTTCCGGCAGCTCGGCGGAAGAAATTTCACCTAAAAGCAAAACCGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTcgc < 2:12476/149‑1 (MQ=255) tCTTCCGGCAGCTCGGCGGAAGAAATTTCACCTAAAAGCAAATCCGGATTGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACTCACAGCACTTCGCTGCTCGAGGa < 7:103479/149‑1 (MQ=255) tCTTCCGGCAGCTCGGCGGAAGAAATTTCACCTAAAAGCAAAACCGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACTCACAGCACTTCGCGGCTCGAGGa < 2:74290/149‑1 (MQ=255) cTTCCGGCAGCTCGGCGGAAGAAATTTCACCTAAAAGCAAAACCGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACTCACAGCACTTCGCGGCTCGAGGa > 1:26262/1‑148 (MQ=255) ggcggAAGAAATTTCACCTAAAAGCAAAACCGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCg > 5:239864/1‑149 (MQ=255) aagaaATTTCACCTAAAAGCAAAACCGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACTCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCctgc > 1:122252/1‑149 (MQ=255) gaaATTTCACCTAAAAGCAAAACCGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACTCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCctgctg < 2:122252/149‑1 (MQ=255) gaaATTTCACCTAAAAGCAAAACCGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCct < 6:31890/145‑1 (MQ=255) gaaATTTCACCTAAAAGCAAAACCGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCct > 5:31890/1‑145 (MQ=255) cTAAAAGCAAAACCGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACTCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCAtgg < 2:26262/149‑1 (MQ=255) cTAAAAGCAAAACCGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCGTG‑TGCCAGCCAtgac > 4:176403/1‑147 (MQ=255) cAAAACCGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGCCATGACTGGcag < 6:239864/149‑2 (MQ=255) gaaggaaCTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCCCGGTGCCGATGAGCACTCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAgg < 2:154590/149‑1 (MQ=255) gaaggaaCTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCCGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAgg < 1:220259/149‑1 (MQ=255) gaaCTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGttt > 3:185473/1‑148 (MQ=255) ccAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAgcacgca > 6:165054/1‑69 (MQ=255) ccAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAgcacgca < 5:165054/69‑1 (MQ=255) ccAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACTCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCCGTCATGGCTGGCACCCGCGAGGTTGTCTTCGCCgc > 8:169701/1‑148 (MQ=255) aGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACTAACAGAACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAgg < 7:169701/149‑1 (MQ=255) aCTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACTCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAg > 5:92427/1‑149 (MQ=255) aCTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAg > 3:101008/1‑149 (MQ=255) ttAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACTCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGcaccac > 7:27014/1‑149 (MQ=255) agcagcCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGCGCACTCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCg > 2:88531/1‑148 (MQ=255) gcagcCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACTCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAg < 5:110313/130‑1 (MQ=255) gcagcCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACTCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAg > 6:110313/1‑130 (MQ=255) gcCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACTCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAgg < 6:92427/149‑1 (MQ=255) ccAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGca > 5:45677/1‑136 (MQ=255) ccAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGca < 6:45677/136‑1 (MQ=255) aaGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACTCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTcgc > 4:237288/1‑149 (MQ=255) aGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGG‑GGCGCGCGCTgtc < 4:101008/149‑1 (MQ=255) cATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTgtcg > 2:94512/1‑149 (MQ=255) cATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTgtcg < 1:94512/149‑1 (MQ=255) tGCCGATGAGCACTCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAgg < 2:126816/119‑1 (MQ=255) tGCCGATGAGCACTCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAgg > 1:126816/1‑119 (MQ=255) acgcacAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCtt > 3:207511/1‑149 (MQ=255) cgcacAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTg < 4:185473/149‑1 (MQ=255) | AAAGCACCCGGCGGCGAAGGTCTTCCGGCAGCTCGGCGGAAGAAATTTCACCTAAAAGCAAAACCGGAAGGAACTCATCCCAGGTAGCCACTGTCTTAAGCAGCCCAAGCAGATTGCCCGGAGCATTCACGGTGCCGATGAGCACGCACAGCACTTCGCGGCTCGAGGACAATGGCTCGACCGCCTGCTGCCAGTCATGGCTGGCACACGCGAGGTTTTCTTCGCCGAGGAAGTTCAGCACCACCGCAAGGTCGCGGCGGCGCGCGCTGTCGTCATCGATCAGCAGAATCTTG > NC_002947/4965051‑4965343 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |