| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,022,462 | G→A | 10.8% | V183V (GTC→GTT) | PP_4427 ← | amino acid ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,022,462 | 0 | G | A | 10.8% | 97.3 / 3.0 | 37 | V183V (GTC→GTT) | PP_4427 | amino acid ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base G (16/17); new base A (2/2); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.19e-01 | |||||||||||
AAGAGCTGTATTGCATAGTTCCAGTCGAAGAAATTCATCACAGGCTCCCTTGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGCGTTATAAGGGAAACAAGGGATGTGTTTTTCAACAGCTCGATCAGCAGATTAGTTCCAGGCCCCATCGCTGCGAG > NC_002947/5022317‑5022599 | aaGAGCTGTATTGCATAGTTCCAGTCGAAGAAATTCATCACAGGCTCCCTTGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGAcc < 8:6870/149‑1 (MQ=255) cTGTATTGCATAGTTCCAGTCGAAGAAATTCATCACAGGCTCCCTTGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCGGCGCTAGGGCGAAGACCtt > 6:66982/1‑146 (MQ=255) gCATAGTTCCAGTCGAAGAAATTCATCACAGGCTCCCTTGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGtctc > 4:122102/1‑148 (MQ=255) gCATAGTTCCAGTCGAAGAAATTCATCACAGGCTCCCTTGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGtctc > 3:171723/1‑148 (MQ=255) gCATAGTTCCAGTCGAAGAAATTCATCACAGGCTCCCTTGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGtctc < 2:148260/148‑1 (MQ=255) gaagaaATTCATCACAGGCTCCCTTGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATc > 1:105918/1‑149 (MQ=255) aTTCATCACAGGCTCCCTTGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTg > 1:145219/1‑149 (MQ=255) atcaCAGGCTCCCTTGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGt < 1:130459/121‑1 (MQ=255) atcaCAGGCTCCCTTGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGt > 2:130459/1‑121 (MQ=255) acaGGCTCCCTTGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGcc > 6:19086/1‑149 (MQ=255) caGGCTCCCTTGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCtt < 2:725/111‑1 (MQ=255) caGGCTCCCTTGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCtt > 1:725/1‑111 (MQ=255) gCTCCCTTGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGa < 3:122102/149‑1 (MQ=255) ccTTGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCt > 1:151338/1‑149 (MQ=255) ccTTGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCt > 1:245798/1‑149 (MQ=255) ttGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTc > 8:30561/1‑148 (MQ=255) ggACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCa < 2:145219/147‑1 (MQ=255) gACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCTATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCt < 8:14745/98‑1 (MQ=255) gACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCAATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCt > 7:14745/1‑98 (MQ=255) cACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGc < 2:31339/149‑1 (MQ=255) cACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGc < 4:171723/149‑1 (MQ=255) cGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGCGt < 5:107889/149‑1 (MQ=255) gggCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCGGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGCGtt < 5:66982/149‑1 (MQ=255) tGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGCGTTATAAGGGa < 1:93309/149‑1 (MQ=255) tAAACGCCGCATTGCCAAGTTAATCGCTTGGTCCATTACGAAGTACATCAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGCGTTATAAGGGAAACAAg < 4:42454/147‑1 (MQ=255) aaCGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGCGTTATAAGGGAAACAAGGGa < 2:245798/148‑1 (MQ=255) ttGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGCGTTATAAGGGAAACAAGGGATGTGTTTTTc < 7:191312/149‑1 (MQ=255) ccAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAAACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGCGTTATAAGGGAAACAAGGGATGTGtt < 6:57332/142‑1 (MQ=255) ccAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAAACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGCGTTATAAGGGAAACAAGGGATGTGtt < 6:53528/142‑1 (MQ=255) ccAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAAACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGCGTTATAAGGGAAACAAGGGATGTGtt > 5:57332/1‑142 (MQ=255) ccAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAAACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGCGTTATAAGGGAAACAAGGGATGTGtt > 5:53528/1‑142 (MQ=255) tCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGCGTTATAAGGGAAACAAGGGATGTGTTTTTCAACAGCTCGATc < 7:30561/149‑1 (MQ=255) ttGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGCGTTATAAGGGAAACAAGGGATGTGTTTTTCAACAGCTCGATcag > 3:175994/1‑147 (MQ=255) cagcGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGCGTTATAAGGGAAACAAGGGATGTGTTTTTCAACAGCTCGATCAGCAGATTAGTTCCAGGCCCCATCGCTg > 5:3814/1‑149 (MQ=255) cgcTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGCGTTATAAGGGAAACAAGGGATGTGTTTTTCAACAGCTCGATcag < 4:5188/120‑1 (MQ=255) cgcTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGCGTTATAAGGGAAACAAGGGATGTGTTTTTCAACAGCTCGATcag > 3:5188/1‑120 (MQ=255) gcTAGGGCGAAGACCTTGAGAGACTCGAATGTAGCCTGATCGAGTTCGCGCGCCCTGAAGCTCAAATCTGCAAGCGTAATAAGGGAAACAAGGGATGTGTTTTTCAACAGCTCGATCAGGCGATTAGTTGCAGGCCCCATCGCAGCGAg > 6:74964/1‑149 (MQ=255) | AAGAGCTGTATTGCATAGTTCCAGTCGAAGAAATTCATCACAGGCTCCCTTGGACACGACCACGGGCCAGATGTCGCTCTAAACGCCGCATTGCCAAGTTAATCGCTTGGGCCATTACGAAGTACATGAGCAGCGCTAGGGCGAAGACCTTGAGAGTCTCGAATGTAGCCTGATCGAGTTGGCGCGCCCTGAAGCTCAAATCTGCAAGCGTTATAAGGGAAACAAGGGATGTGTTTTTCAACAGCTCGATCAGCAGATTAGTTCCAGGCCCCATCGCTGCGAG > NC_002947/5022317‑5022599 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |