Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 362,375 C→G 5.8% L44L (CTG→CTC) ‡ PP_0301 ← betainyl‑CoA thiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947362,3750CG 5.8% 222.7 / 3.6 69L44L (CTG→CTC) ‡PP_0301betainyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base C (32/33);  new base G (2/2);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

ACACGTGAAGACGCTTGCGATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTGGTCTCCTTAA  >  NC_002947/362233‑362520
                                                                                                                                                 |                                                                                                                                                 
acacGTGAAGACGCTTGCGATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGcc                                                                                                                                              >  3:216417/1‑148 (MQ=255)
    gTGAAGACGCTTGCGATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGc                                                                                                                                         >  4:233266/1‑149 (MQ=255)
       aaGACGCTTGCGATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGt                                                                                                                                      <  3:233266/149‑1 (MQ=255)
            gCTTGCGATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGCAAAATGAGATTAATATGCCCAACCAGCTTAAAAACCAATTAACCGATTTCCCCCCGGATAAGGGGGACCAGCCCGATGCggac                                                                                                                                     >  1:218787/1‑146 (MQ=255)
              ttGCGATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCAAt                                                                                                                                  >  6:38856/1‑146 (MQ=255)
                  gATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCCTGGACGCCGAtag                                                                                                                                         <  8:240671/135‑3 (MQ=255)
                  gATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCCTGGACGCCGAtag                                                                                                                                         >  7:240671/1‑133 (MQ=255)
                   aTCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCAt                                                                                                                          <  7:129697/149‑1 (MQ=255)
                      aaaGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCCTGGACGCCGAtagc                                                                                                                                        <  1:197145/132‑4 (MQ=255)
                      aaaGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCCTGGACGCCGAtagc                                                                                                                                        >  2:197145/1‑129 (MQ=255)
                       aaGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGt                                                                                                                      >  1:247325/1‑149 (MQ=255)
                         gCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTgg                                                                                                                    >  3:212543/1‑149 (MQ=255)
                            gAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCg                                                                                                                  >  3:126615/1‑148 (MQ=255)
                            gAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGt                                                                                                                 <  5:15981/149‑1 (MQ=255)
                              ggATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTa                                                                                                                <  4:33287/148‑1 (MQ=255)
                               gATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGc                                                                                                              <  4:260963/149‑1 (MQ=255)
                                           cACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATcag                                                                                                    <  4:98544/147‑1 (MQ=255)
                                           cACCCACACCTCCGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTCAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATcag                                                                                                    <  4:94756/147‑1 (MQ=255)
                                              ccACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCaga                                                                                                                              <  1:7271/118‑1 (MQ=255)
                                              ccACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCaga                                                                                                                              >  2:7271/1‑118 (MQ=255)
                                              ccACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATcagc                                                                                                   <  4:247225/145‑1 (MQ=255)
                                              ccACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATcagc                                                                                                   >  3:247225/1‑145 (MQ=255)
                                              ccACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAgg                                                                                                >  6:7874/1‑148 (MQ=255)
                                              ccACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGCTGGCGTAGCTGAAGATCAGCAgg                                                                                                >  2:20665/1‑148 (MQ=255)
                                              ccACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATAGGTTGCGTAGCTGAAGATCAGCAgg                                                                                                >  4:169572/1‑148 (MQ=255)
                                                   ccTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAg                                                                                          >  1:81873/1‑149 (MQ=255)
                                                    cTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCgg                                                                                                                                       >  6:244088/1‑103 (MQ=255)
                                                    cTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAgg                                                                                         >  5:189061/1‑149 (MQ=255)
                                                       ggTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCACCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCa                                                                                       <  5:232293/148‑1 (MQ=255)
                                                           cccAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCACCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATcgcg                                                                                  >  2:21854/1‑149 (MQ=255)
                                                            ccAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGTCCGATGCCGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATagcg                                                                                  >  2:50742/1‑148 (MQ=255)
                                                            ccAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTa                                                                                              >  1:253827/1‑136 (MQ=255)
                                                            ccAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTa                                                                                              <  2:253827/136‑1 (MQ=255)
                                                              aGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAg                                                                               <  4:212543/149‑1 (MQ=255)
                                                                cTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGt                                                                             <  2:24964/149‑1 (MQ=255)
                                                                       tCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTATGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGtt                                                                      >  1:202182/1‑149 (MQ=255)
                                                                          tGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGt                                                                    >  6:4929/1‑148 (MQ=255)
                                                                           gC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGa                                                                                                           >  4:48658/1‑108 (MQ=255)
                                                                           gC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGa                                                                                                           <  3:48658/108‑1 (MQ=255)
                                                                            c‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTaa                                                                  <  4:126615/148‑1 (MQ=255)
                                                                              aaaT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATc                                                                <  6:151740/149‑1 (MQ=255)
                                                                              aaaT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATc                                                                <  6:189061/149‑1 (MQ=255)
                                                                                 t‑a‑atTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAg                                                                                          >  4:215506/1‑120 (MQ=255)
                                                                                 t‑a‑atTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAg                                                                                          <  3:215506/120‑1 (MQ=255)
                                                                                     atTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCaga                                                                                                                              <  8:189209/82‑1 (MQ=255)
                                                                                     atTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCaga                                                                                                                              >  7:189209/1‑82 (MQ=255)
                                                                                     atTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGAcc                                                            <  2:218787/148‑1 (MQ=255)
                                                                                                      gCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCgtg                                          <  4:216417/149‑1 (MQ=255)
                                                                                                       cGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCgtgg                                         >  2:110743/1‑149 (MQ=255)
                                                                                                          gAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGc                                      >  6:147045/1‑149 (MQ=255)
                                                                                                                   gTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGa                             >  3:4977/1‑149 (MQ=255)
                                                                                                                    ttACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGGGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGAt                            <  3:169572/149‑1 (MQ=255)
                                                                                                                            ttGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCACGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCgg                    <  2:247325/149‑1 (MQ=255)
                                                                                                                             tGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGTATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCgg                    <  2:81873/148‑1 (MQ=255)
                                                                                                                             tGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCgg                    <  5:147045/148‑1 (MQ=255)
                                                                                                                               ccggcgGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGTAGATCAGCAGGTTGAAGGGATCGCGCAGGTGGCCGTTGTAATCGTCGCTGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCa                 <  1:50742/146‑1 (MQ=255)
                                                                                                                               cccGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCa                 >  5:215577/1‑149 (MQ=255)
                                                                                                                                ccGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCgg                                    >  2:226182/1‑129 (MQ=255)
                                                                                                                                ccGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCgg                                    <  1:226182/129‑1 (MQ=255)
                                                                                                                                ccGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCAt                >  1:267342/1‑149 (MQ=255)
                                                                                                                                   cgGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCtt             <  5:7874/149‑1 (MQ=255)
                                                                                                                                    tgCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATATGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCGTTTGTAATCTACCCAGTCCTCCTGGACCGTGGTTCGGTAGGTGATCAGAGCGGGCATCTTg            <  1:20665/148‑1 (MQ=255)
                                                                                                                                       tATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTGGtc         >  3:133549/1‑149 (MQ=255)
                                                                                                                                          cGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTg            <  6:205705/143‑1 (MQ=255)
                                                                                                                                          cGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTg            >  5:205705/1‑143 (MQ=255)
                                                                                                                                          cGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTGGTCTcc      >  1:31587/1‑149 (MQ=255)
                                                                                                                                           ggCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTGGTCTcc      <  5:4929/148‑1 (MQ=255)
                                                                                                                                           ggCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTGGTCTcc      <  1:110743/148‑1 (MQ=255)
                                                                                                                                              gTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTGGTCTCCTTaa  <  2:202182/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
ACACGTGAAGACGCTTGCGATCAAAGCCGAGGATCTGTGTCTGCACCCACACCTCGGTGCCCAGCTTCACCTCGTGC‑AAAT‑A‑ATTGATATGCGCCTCCAGCGTGAACAGCGAGTTACCGCTTTGCCCGCGGCTATCGGCGTCCAGGCCGATGCGGTCCATCAGAGCATCGGTGGCGTAGCTGAAGATCAGCAGGTAGAAGGCATCGCGCAGGTGGCCGTTGTAATCGACCCAGTCCTCCTGGACCGTGGTGCGGTAGGTGATCAGAGCGGGCATCTTGGTCTCCTTAA  >  NC_002947/362233‑362520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑