Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,029,418 G→A 9.1% L19L (TTG→TTA)  PP_4433 → amino acid MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,029,4180GA 9.1% 152.2 / 2.2 54L19L (TTG→TTA) PP_4433amino acid MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (24/25);  new base A (3/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATAAAGCGCAGATCAGCAGTTGGTCATTTGGCCATGTGCATTCAAGCCTGACTAGCCAGTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCTCTCAATCTGAAGCCCTTTACTTCGGC  >  NC_002947/5029272‑5029565
                                                                                                                                                   |                                                                                                                                                   
cgATCAAGCGCAGATCAGCAGTTTGTCATCTGGCCATGTGCATTCAAGCCTGACTAGCCAGTTCTTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAACAGCTTGaa                                                                                                                                                   <  2:3899/149‑1 (MQ=255)
              tCAGCAGTTTGTCATTTGGCCATGTGCATTCAAGCCTGACTAGACAGTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTc                                                                                                                                     <  5:224797/149‑1 (MQ=255)
                  cagTTGGTCATTTGGCCATGTGCATTCAAGCCTGACTAGCCAGTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTTCGCATGCAGCTTCAACCAGTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCaa                                                                                                                                 >  4:163376/1‑149 (MQ=255)
                        gTCATTTGGCCATGTGCATTCAAGCCTGACTAGCCAGTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATTAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGt                                                                                                                           >  4:60206/1‑149 (MQ=255)
                               ggCCATGTGCATTCAAGCCTGACTAGCCAGTTCGTCTTACCTAAGNAAAGTGGTAAATTATGTCTAT‑TAGTACGCATACAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTAAACATTAGCATGCCTCGCAAAGCCGTGGtatc                                                                                                                     >  1:192606/1‑145 (MQ=255)
                               ggCCATGTGCATTCAAGCCTGACTAGCCAGTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGc                                                                                                                     <  5:54278/148‑1 (MQ=255)
                               ggCCATGTGCATTCAAGCCTGACTAGCCAGTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTAAACATGAGCATGCCTCGCAAAGCCGTGGTCg                                                                                                                      >  1:188835/1‑147 (MQ=255)
                                 ccATGTGCATTCAAGCCTGACTAGCCAGTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACAt                                                                                                                                                <  7:101569/119‑1 (MQ=255)
                                 ccATGTGCATTCAAGCCTGACTAGCCAGTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACAt                                                                                                                                                >  8:101569/1‑119 (MQ=255)
                                  cATGTGCATTCAAGCCTGACTAGCCAGTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGGCGCAGCt                                                                                                                 <  8:213687/149‑1 (MQ=255)
                                        cATTCAAGCCTGACTAGCCAGTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCAtt                                                                                                            <  7:69064/148‑1 (MQ=255)
                                          ttCAATCCTGACTAGCCAGTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTGGCAAAGCCGTGGTCGCAGCTGCATTAgg                                                                                                         <  1:153624/149‑1 (MQ=255)
                                           tCAAGCCTGACTAGCCAGTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAgg                                                                                                         >  4:184351/1‑148 (MQ=255)
                                           tCAAGCCTGACTAGCCAGTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTAAACCATTTCTTCGAGCACAATAAAAAGCTTAAAAATGAGCATGCCTCGCAAAGCCGAGGTCGCAGATGCATAAgg                                                                                                         >  3:244595/1‑148 (MQ=255)
                                                 cTGACTAGCCAGTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAAcgc                                                                                                   >  4:23339/1‑148 (MQ=255)
                                                         ccAGTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGc                                                                                                                                   <  8:133086/108‑1 (MQ=255)
                                                         ccAGTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGc                                                                                                                                   >  7:133086/1‑108 (MQ=255)
                                                            gTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGtt                                                                                       <  3:183678/149‑1 (MQ=255)
                                                            gTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGtt                                                                                       <  3:184351/149‑1 (MQ=255)
                                                                  cTTACCTAAGATAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTa                                                                                 >  7:216646/1‑149 (MQ=255)
                                                                        tAAGAAAAGTGGTAAATTATGTCTATGTAGTACGCATACAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGaa                                                                            >  1:3107/1‑149 (MQ=255)
                                                                        tAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTAc                                                                                <  1:260492/144‑1 (MQ=255)
                                                                        tAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTAc                                                                                >  2:260492/1‑144 (MQ=255)
                                                                        tAAGAAAAGTGCTAAATTATGACTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCATCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACCCCACGCAATCGTTCGAGTACGTAAt                                                                           >  6:274725/1‑149 (MQ=255)
                                                                            aaaaGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGAt                                                                                   >  6:270860/1‑137 (MQ=255)
                                                                            aaaaGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGAt                                                                                   <  5:270860/137‑1 (MQ=255)
                                                                             aaaGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATg                                                                      <  1:126286/149‑1 (MQ=255)
                                                                                gTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGGGACGGAATGGTGCGATTACGGAATCTATGCtt                                                                   <  1:169593/149‑1 (MQ=255)
                                                                                   gTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGaa                                                                                            <  6:85129/121‑1 (MQ=255)
                                                                                   gTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGATTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGaa                                                                                            >  5:85129/1‑121 (MQ=255)
                                                                                      aaTTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACggg                                                              <  8:216646/148‑1 (MQ=255)
                                                                                                     gTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTc                                                     <  3:240914/143‑1 (MQ=255)
                                                                                                     gTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTc                                                     >  4:240914/1‑143 (MQ=255)
                                                                                                     gTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCAtct                                                 >  8:57016/1‑147 (MQ=255)
                                                                                                        cGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAgcc                                            <  3:23339/149‑1 (MQ=255)
                                                                                                         gCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAgg                                                                                                         <  6:135253/87‑1 (MQ=255)
                                                                                                           aTCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAgccgcc                                         >  4:240404/1‑149 (MQ=255)
                                                                                                             ccAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCtt                                       >  4:248490/1‑149 (MQ=255)
                                                                                                                    cAACCATTTCTTCGATCACAATAAAATGCTTGATCATGAGCATGCCTCGCAAAGCCGTGGTCGCAGGTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGttt                                   <  4:244595/146‑1 (MQ=255)
                                                                                                                     aaCCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCt                                <  3:60206/148‑1 (MQ=255)
                                                                                                                     aaCCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTg                               <  2:3107/149‑1 (MQ=255)
                                                                                                                     aaCCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTg                               >  2:121636/1‑149 (MQ=255)
                                                                                                                     aaCCATTTCTTCGAGCACAATAAAAAGCTTAAACATGAGCATGCCTCTCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCt                                <  2:188835/148‑1 (MQ=255)
                                                                                                                     aaCCATTTCTTCGAGAACAATAAAAAGCTTAAACATGAGCATTCCTCGCAAAGCCGTTGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCt                                <  2:192606/148‑1 (MQ=255)
                                                                                                                      aCCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGCCAACGCGACGGATAGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCAACTCACCCGACTTCTTTCCAgg                              >  4:254680/1‑149 (MQ=255)
                                                                                                                          tttCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGTTCACCTTCATCTCAGCCGCCTTGTTTCCTGGctct                          >  8:71258/1‑149 (MQ=255)
                                                                                                                             cttcGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCTCTCaa                       >  3:242441/1‑149 (MQ=255)
                                                                                                                                   ggacaATAAAAAGCTTGAAGATGAGCATGCCTCGCAAAGCCGTGGTCGGAGCTGCATTAGGCAACGCGTCGGAATGGTTCGATTACGGAATCTATGCTTTCGGGCTCACGTTCATCTCAGCCGCCTTGTTTCCTGGCTCTCAATCTGaa                 <  5:274725/147‑1 (MQ=255)
                                                                                                                                   gCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCTCTCAATCTg                   >  3:87610/1‑147 (MQ=255)
                                                                                                                                      caATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCTCTCAATCTGAAGcc              >  4:121122/1‑149 (MQ=255)
                                                                                                                                        aTAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCTCTCAATCTGAAGCCCt            <  3:121122/149‑1 (MQ=255)
                                                                                                                                         tAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCTCTCAATCTGAAGCCCtt           <  3:254680/149‑1 (MQ=255)
                                                                                                                                           aaaaGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCTCTCAATCTGAAGCCCTTTa         >  7:2769/1‑149 (MQ=255)
                                                                                                                                                  tGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCTCTCAATCTGAAGCCCTTTACTTCTGc  >  8:96143/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGATAAAGCGCAGATCAGCAGTTGGTCATTTGGCCATGTGCATTCAAGCCTGACTAGCCAGTTCGTCTTACCTAAGAAAAGTGGTAAATTATGTCTAT‑TAGTACGCATCCAGCTTCAACCATTTCTTCGAGCACAATAAAAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCTCTCAATCTGAAGCCCTTTACTTCGGC  >  NC_002947/5029272‑5029565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑