Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,047,262 C→A 8.8% intergenic (+243/+205) PP_5683 → / ← PP_5684 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,047,2620CA 8.8% 166.2 / 6.8 57intergenic (+243/+205)PP_5683/PP_5684hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/23);  new base A (2/3);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01

CGCTTGCCTTTCGGAGTATCCATCAACTTCCACCGAGTCATCTCAGATACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGAAAAGCGTTGAAGTGACGTTGCTGCCC  >  NC_002947/5047122‑5047407
                                                                                                                                            |                                                                                                                                                 
cGCTTGCCTTTCGGTGTATCCATCAACTTCAACCGAGTCATCTCAGATACTCTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCTAGCAAGGGGAATGATATGAAGGCCTCTGATGc                                                                                                                                           <  2:93625/149‑1 (MQ=255)
cGCTTGCCTTTCGGAGTATCCATCAACTTCCACCGAGTCATCTCAGATACACTTGGAGTCAGGAAGCTTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGAt                                                                                                                                             <  5:154398/147‑1 (MQ=255)
 gCTTGCCTTTCGGAGTATCCATCAACTTCCACCGAGTCATCTCAGATACACTTGGCGTCAGGAAGGGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCTGGCCTCTGATGcc                                                                                                                                          <  2:182769/149‑1 (MQ=255)
     gCCTTTCGGAGTATCCATCAACTTCCACCGAGTCATCTCAGATACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGc                                                                                                                                       >  3:228492/1‑148 (MQ=255)
      ccTTTCGGAGTATCCATCAACTTCCACCGAGTCATCTCAGATACACTTGGCGTCAGGATGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCg                                                                                                                                      <  1:167661/148‑1 (MQ=255)
      ccTTTCGGAGTATCCATCAACTTCCACCGAGTCATCTCAGATACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGa                                                                                                                                     <  6:73897/149‑1 (MQ=255)
       cTTTCGGAGTATCCATCAACTTCCACCGAGTCATCTCAGATACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCATCAGAGGCCTGctta                                                                                                                                    <  3:256769/149‑4 (MQ=255)
        tttCGGAGTATCCATCAACTTCCACCGAGTCATCTCAGATACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGAcc                                                                                                                                   >  2:242544/1‑149 (MQ=255)
          tCGGAGTATCCATCAACTTCCACCGAGTCATCTCAGATACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTACGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGAcc                                                                                                                                   <  4:193398/147‑1 (MQ=255)
              aGTATCCATCAACTTCCACCGAGTCATCTCAGATACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTaaaa                                                                                                                             <  6:133193/149‑1 (MQ=255)
                  tCCATCAACTTCCACCGAGTCATCTCAGATACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTgg                                                                                                                         <  1:242544/149‑1 (MQ=255)
                     aTCAACTTCCACCGAGTCATCTCAGAAACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCg                                                                                                                      >  1:6338/1‑149 (MQ=255)
                           ttCCACCGAGTCATCTCAGATACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCgtttgcg                                                                                                                >  3:263439/1‑143 (MQ=255)
                           ttCCACCGAGTCATCTCAGATACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAAc                                                                                                                 >  8:189847/1‑148 (MQ=255)
                                    gTCATCTCAGATACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCtt                                                                                                       >  8:130048/1‑149 (MQ=255)
                                       aTCTCAGATACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCAACAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCt                                                                                                    >  2:209928/1‑149 (MQ=255)
                                          tcAGATACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGaa                                                                                                 >  3:64749/1‑149 (MQ=255)
                                            aGATACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGa                                                                                                  <  7:130048/146‑1 (MQ=255)
                                             gATACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCa                                                                                              <  7:189847/149‑1 (MQ=255)
                                               tACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAg                                                                                             <  4:228492/148‑1 (MQ=255)
                                                  acTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGaca                                                                                         >  7:15167/1‑149 (MQ=255)
                                                     tGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGCGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGc                                                                                      >  2:186399/1‑149 (MQ=255)
                                                         gTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGa                                                                                  <  8:66525/149‑1 (MQ=255)
                                                               aaGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGc                                                                                      >  5:167912/1‑139 (MQ=255)
                                                               aaGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGc                                                                                      <  6:167912/139‑1 (MQ=255)
                                                                       gCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCAt                                                                     >  2:51478/1‑148 (MQ=255)
                                                                        cACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATg                                                                    >  1:179067/1‑148 (MQ=255)
                                                                                  gTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGAAt                                                         <  1:51478/149‑1 (MQ=255)
                                                                                  gTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGAAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCATTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGAt                                                         >  3:136407/1‑149 (MQ=255)
                                                                                   ttCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGAt                                                         >  4:260551/1‑148 (MQ=255)
                                                                                              gCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAagcag                                             >  2:77184/1‑149 (MQ=255)
                                                                                                ccACCTATGACCACAATACAGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGAAGAACTAAAGCAGCt                                           >  5:116626/1‑149 (MQ=255)
                                                                                                         aCCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGt                                       <  8:250964/144‑1 (MQ=255)
                                                                                                         aCCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGt                                       >  7:250964/1‑144 (MQ=255)
                                                                                                         aCCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTcgc                                   <  4:64749/148‑1 (MQ=255)
                                                                                                                tACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGa                            >  3:139777/1‑148 (MQ=255)
                                                                                                                  ccGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGaaaa                         <  8:15167/149‑1 (MQ=255)
                                                                                                                   cGAGCAAGGGGAATGATAAGCAGGGCTCTGATGCGTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGAAAAg                        <  6:116626/149‑1 (MQ=255)
                                                                                                                   cGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGAAAAg                        >  6:270910/1‑149 (MQ=255)
                                                                                                                      gCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGaa                                                      >  2:204676/1‑116 (MQ=255)
                                                                                                                      gCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGAAAAGCGt                     >  5:163414/1‑149 (MQ=255)
                                                                                                                                tGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGAAAAGCGTTGAAGTGACg           >  2:60705/1‑149 (MQ=255)
                                                                                                                                tGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGAAAAGCGTTGAAGTGACg           >  6:204822/1‑149 (MQ=255)
                                                                                                                                 gATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAAGATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCTGGAAAAGCGTTGAAGTGACGt          >  8:105225/1‑149 (MQ=255)
                                                                                                                                 gATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCATTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTCGGTTCGCGCCAGGAAAAGCGTTGAAGTGACGt          >  1:206936/1‑149 (MQ=255)
                                                                                                                                   tAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGAAAAGCGtt                    >  2:89614/1‑137 (MQ=255)
                                                                                                                                   tAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGAAAAGCGtt                    >  8:100372/1‑137 (MQ=255)
                                                                                                                                   tAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGAAAAGCGtt                    <  7:100372/137‑1 (MQ=255)
                                                                                                                                   tAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGAAAAGCGtt                    <  1:89614/137‑1 (MQ=255)
                                                                                                                                     aGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGTGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGAAAAGCGTTGAAGTGACGTtgct      <  1:209928/149‑1 (MQ=255)
                                                                                                                                     aGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGAAAAGCGTTGAAGTGACGTtgc       <  4:139777/148‑1 (MQ=255)
                                                                                                                                      gCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGAAAAGCGTTGAAGTGACGTtgctg     >  6:133847/1‑149 (MQ=255)
                                                                                                                                        aGGCATCAGAGGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAAc                                                     >  1:106740/1‑99 (MQ=255)
                                                                                                                                        aGGCATCAGAGGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAAc                                                     <  2:106740/99‑1 (MQ=255)
                                                                                                                                        aGGCATCAGAGGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGAAAAGCGTTGaa                 <  6:35200/135‑1 (MQ=255)
                                                                                                                                        aGGCATCAGAGGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGAAAAGCGTTGaa                 >  5:35200/1‑135 (MQ=255)
                                                                                                                                         ggCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGAAAAGCGTTGAAGTGACGTTGCTGccc  <  6:163414/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
CGCTTGCCTTTCGGAGTATCCATCAACTTCCACCGAGTCATCTCAGATACACTTGGCGTCAGGAAGCGTCGGCACGACAACCGTTCCATCAAGGGCCCACCTATGACCACAATACCGAGCAAGGGGAATGATAAGCAGGCCTCTGATGCCTGCGACCGTAAAATTGGTCGCAAACGACCTGCCTTTCTGAATCAGGACACGCTTGACCGCATTGCCCATGAACATGCGGATGAACTAAAGCAGCTGGGTTCGCGCCAGGAAAAGCGTTGAAGTGACGTTGCTGCCC  >  NC_002947/5047122‑5047407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑