| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,078,877 | G→C | 13.2% | intergenic (‑122/+275) | mgtE ← / ← PP_t56 | magnesium transporter/tRNA‑Arg |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,078,877 | 0 | G | C | 13.2% | 137.5 / 12.7 | 53 | intergenic (‑122/+275) | mgtE/PP_t56 | magnesium transporter/tRNA‑Arg |
| Reads supporting (aligned to +/- strand): ref base G (24/22); new base C (3/4); total (27/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01 | |||||||||||
TTTTTTAACTTCTACTTCGGTCATAGGCGAACTCCACTCCCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGAAGCTATCAGTGCGACGATTGTTGTGGATGGGAAAGCACATTTGAT > NC_002947/5078732‑5079022 | ttttttAACTTCTACTTCGGTCATAGGCGAACTCCACTCCCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCcggcgg > 7:12098/1‑149 (MQ=255)ttttttAACTTCTACTTCGGTCATAGGCGAACTCCACTCCCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCcggcgg > 3:199060/1‑149 (MQ=255) tttttAACTTCTACTTCGGTCATAGGCGAACTCCACTCCCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGCCggg > 3:38336/1‑149 (MQ=255) ttttAACTTCTACTTCGGTCATAGGCGAACTCCACTCCCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCcggcgg < 7:62314/147‑1 (MQ=255) tttAACTTCTACTTCGGTCATAGGCGAACTCCACTCCCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGc > 6:81157/1‑149 (MQ=255) cTTCTACTTCGGTCATAGGCGAACTCCACTCCCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGCCGGGGCttgtg < 4:38336/149‑5 (MQ=255) ttCTACTTCGGTCATAGGCGAACTCCACTCCCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACgg > 5:215645/1‑149 (MQ=255) cTACTTCGGTCATAGGCGAACTCCACTCCCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACgg < 5:81157/147‑1 (MQ=255) cTACTTCGGTCATAGGCGAACTCCACTCCCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACgg < 3:181549/147‑1 (MQ=255) gTCATAGGCGAACTCCACTCCCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCAt < 8:12098/149‑1 (MQ=255) aTAGGCGAACTCCACTCCCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATaca > 1:3504/1‑149 (MQ=255) aTAGGCGAACTCCACTCCCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATac < 1:90435/148‑1 (MQ=255) gCGAACTCCACTCCCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGCCGGGGCttgt > 2:261046/1‑127 (MQ=255) gCGAACTCCACTCCCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGCCGGGGCttgt < 1:261046/130‑4 (MQ=255) aaCTCCACTCCCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTaa < 3:81490/149‑1 (MQ=255) cactccCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATac < 7:40709/136‑1 (MQ=255) cactccCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATa > 3:251434/1‑135 (MQ=255) cactccCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATa < 4:251434/135‑1 (MQ=255) cactccCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGc < 4:155451/147‑1 (MQ=255) actccCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGt < 6:215645/149‑1 (MQ=255) agcagcGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGt > 3:48823/1‑143 (MQ=255) agcagcGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATc < 4:199060/149‑1 (MQ=255) cagcGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGc > 4:169676/1‑149 (MQ=255) gAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTaaa > 6:218794/1‑149 (MQ=255) cACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAAttt > 6:82278/1‑149 (MQ=255) ccGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCTAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGTCTGACGGGCGCAATAATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTgg < 3:77574/149‑1 (MQ=255) ggAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAg > 3:237822/1‑148 (MQ=255) tcaatcGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACaa < 4:237822/149‑1 (MQ=255) caatcGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGCCGGGGCttg < 8:113552/90‑3 (MQ=255) gTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTg > 7:58777/1‑149 (MQ=255) cAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTgcg > 7:183949/1‑149 (MQ=255) gTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGGATGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATa < 1:51992/86‑1 (MQ=255) gTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATa > 2:51992/1‑85 (MQ=255) tATGTATCGTGTTAGCGAGTTACTACTTTGTAATTCCATGG‑TTTTTTTCCACAAGCCCCGGGGGGGCTGACGGGCGCTATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTg < 4:48823/148‑1 (MQ=255) tGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGa > 5:207669/1‑149 (MQ=255) gAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGa > 1:76598/1‑148 (MQ=255) aTCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACaa > 4:254003/1‑149 (MQ=255) tgtTAGCGAGTAACTACTGGGTAA‑AAAATGG‑TGGTTTTCCACAAGCCCCGGCGGGGAAAACGGGCGCAATAATAAAAAGCTAAAGGTGTAAGATCGGTTAAATTTTTGGCCAGAAAAAAGGTTTGCGGGATAAAGCTGGGAAAACGCGt > 7:168272/1‑149 (MQ=255) ttAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGt > 3:33336/1‑148 (MQ=255) gAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGAAGc > 5:90584/1‑148 (MQ=255) gTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGAAGCTAt < 2:76598/149‑1 (MQ=255) tAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGTGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGAAGCTAt < 5:82278/148‑1 (MQ=255) tAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGAAGCTAt < 2:3504/148‑1 (MQ=255) actactGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGAAGCTTTCAg > 7:225099/1‑149 (MQ=255) actactGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGAAGCTATCa < 4:33336/148‑1 (MQ=255) actactGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGAAGCTATCAg > 3:44478/1‑149 (MQ=255) tCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGAAGCTATCAGTGCGACGAtt > 8:191164/1‑146 (MQ=255) tCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGAAGCTATCAGTGCGACGAtt < 7:191164/146‑1 (MQ=255) tggtTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGAAGCTATCAGTGCGACGATTGTTGTGGATg < 6:90584/149‑1 (MQ=255) gtTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGAAGCTATCAGTGCGACGATTGTTGTGGATgg < 5:218794/148‑1 (MQ=255) tttCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGAAGCTATCAGTGCGACGATTGTTGTGGATGGGa > 8:209550/1‑148 (MQ=255) ccgtcaGCCCCGCCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGAAGCTATCAGTGCGACGATTGTTGTGGATgg < 3:189243/138‑1 (MQ=255) ccgtcaGCCCCGCCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGAAGCTATCAGTGCGACGATTGTTGTGGATgg > 4:189243/6‑143 (MQ=255) cccGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGaa > 6:107982/1‑106 (MQ=255) cccGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGaa < 5:107982/106‑1 (MQ=255) ccGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGAAGCTATCAGTGCGACGATTGTTGTGGATGGGAAAGCACATTTGAt > 8:141162/1‑149 (MQ=255) | TTTTTTAACTTCTACTTCGGTCATAGGCGAACTCCACTCCCAGCAGCGGAGCACGCCGAGGGAATCAATCGGTCAGATCTTTCTGTATGAATCGTGTTAGCGAGTAACTACTGGGTAAGTCCATGG‑TGGTTTTCCACAAGCCCCGGCGGGGCTGACGGGCGCAATCATACACTGCTAAAGCTGTCAGATCGCTTAAATTTTTGGCCAGAACAATCGTTTGCGCGATAAAGCTGGGACAACGCGTGAAGCTATCAGTGCGACGATTGTTGTGGATGGGAAAGCACATTTGAT > NC_002947/5078732‑5079022 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |