Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,090,047 A→G 8.1% L297P (CTG→CCG)  astA‑II ← arginine N‑succinyltransferase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,090,0470AG 8.1% 137.6 / 2.4 49L297P (CTG→CCG) astA‑IIarginine N‑succinyltransferase subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (22/23);  new base G (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

ACGTCTGTTGCCAGACCTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGGCCGCCGTCGAAGATGTCGATG  >  NC_002947/5089900‑5090187
                                                                                                                                                   |                                                                                                                                            
aCGTCTGTTGCCAGACCTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAg                                                                                                                                             <  1:251859/149‑1 (MQ=255)
   tCTGTTGCCAGACCTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGcc                                                                                                                                          >  6:249682/1‑149 (MQ=255)
   tCTGTTGCCAGACCTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGcc                                                                                                                                          <  2:114611/149‑1 (MQ=255)
       ttGCCAGACCTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGtt                                                                                                                                       <  1:86398/148‑1 (MQ=255)
            aGACCTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACGGCCTGTTGcagga                                                                                                                                 <  2:65816/149‑4 (MQ=255)
               ccTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAg                                                                                                                              <  7:101139/149‑1 (MQ=255)
                cTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACAcc                                                                                                                                >  1:171250/1‑146 (MQ=255)
                cTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACAcc                                                                                                                                <  2:171250/146‑1 (MQ=255)
                 tctaaCCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACGGCCTGTTGcagg                                                                                                                                  <  5:96973/140‑3 (MQ=255)
                 tCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACGGCCTGTTGcagg                                                                                                                                  >  6:96973/1‑141 (MQ=255)
                     aCCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACgg                                                                                                                        <  3:150966/149‑1 (MQ=255)
                      ccGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACggg                                                                                                                       >  6:138627/1‑149 (MQ=255)
                       cgcgACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGgc                                                                                                                      >  6:25310/1‑149 (MQ=255)
                       cgcgACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGgc                                                                                                                      >  4:228740/1‑149 (MQ=255)
                              aGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGcgcccgcc                                                                                                               <  2:215509/149‑1 (MQ=255)
                                gcgcACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCtt                                                                                                             >  1:137135/1‑149 (MQ=255)
                                    aCGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGAc                                                                                                         >  7:7991/1‑149 (MQ=255)
                                        tGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCggg                                                                                                     >  7:100538/1‑149 (MQ=255)
                                          gcgcCCTCACCCACGCACAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCCACACCGCGCGGTAGTCGTGCAACAGGCCGTTGCACACCAGGGACGGGCGCCCGCCTTTGACCGGGGc                                                                                                   <  7:217104/149‑1 (MQ=255)
                                              ccTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCctct                                                                                               >  5:109869/1‑149 (MQ=255)
                                                tCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTc                                                                                             >  4:195428/1‑149 (MQ=255)
                                                    ccACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACCCCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATc                                                                                         >  1:45544/1‑149 (MQ=255)
                                                    ccACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTg                                                                                                                                      <  6:32267/104‑1 (MQ=255)
                                                    ccACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTg                                                                                                                                      >  5:32267/1‑104 (MQ=255)
                                                    ccACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATc                                                                                         <  2:45544/149‑1 (MQ=255)
                                                    ccACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATc                                                                                         >  1:38787/1‑149 (MQ=255)
                                                       cGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCggg                                                                                                     <  7:97697/134‑1 (MQ=255)
                                                       cGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCggg                                                                                                     >  8:97697/1‑134 (MQ=255)
                                                         cccAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGTTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGAt                                                                                          <  5:79435/143‑1 (MQ=255)
                                                         cccAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGAt                                                                                          >  6:79435/1‑143 (MQ=255)
                                                             gcgcGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCCTGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCATGTACGGTCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGc                                                                                <  8:253464/149‑1 (MQ=255)
                                                                            ccACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTgg                                                                 >  1:223703/1‑149 (MQ=255)
                                                                             cACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTggg                                                                <  5:25310/149‑1 (MQ=255)
                                                                             cACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTggg                                                                <  8:100538/149‑1 (MQ=255)
                                                                                      gCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGc                                                       <  2:137135/149‑1 (MQ=255)
                                                                                                 tGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCagag                                            >  7:43502/1‑149 (MQ=255)
                                                                                                        cACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGCGCGGATGCCAGAGGTgcgcg                                     >  2:104290/1‑149 (MQ=255)
                                                                                                           ccAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCAt                                  <  6:109869/149‑1 (MQ=255)
                                                                                                                    gtccagCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCg                         >  7:54472/1‑149 (MQ=255)
                                                                                                                     tccagCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCgg                        <  2:38787/149‑1 (MQ=255)
                                                                                                                        agCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGgcc                     <  3:228740/149‑1 (MQ=255)
                                                                                                                         gCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGgccg                    >  4:110751/1‑149 (MQ=255)
                                                                                                                                cgcgcgGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGGCCGCCGTCGa             <  8:54472/149‑1 (MQ=255)
                                                                                                                                  cgcgGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGGCCGCCGTCGAAg           <  1:108107/149‑1 (MQ=255)
                                                                                                                                  cgcgGTAGTCCTGCAACAGGCCGGTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCGCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGGCCGCCGTCGAAg           <  5:138627/149‑1 (MQ=255)
                                                                                                                                         ggtgtGCAACGGCCTGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGGCCGCCGTCGAAGATGTCGa    >  8:164122/5‑149 (MQ=255)
                                                                                                                                            cTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGGCCGCCGTCGAAGATGGCGATg  >  8:243774/1‑148 (MQ=255)
                                                                                                                                             tGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCagag                                            >  6:256875/1‑105 (MQ=255)
                                                                                                                                             tGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCagag                                            <  5:256875/105‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
ACGTCTGTTGCCAGACCTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGGCCGCCGTCGAAGATGTCGATG  >  NC_002947/5089900‑5090187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑