Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,090,049 G→C 7.9% G296G (GGC→GGG) ‡ astA‑II ← arginine N‑succinyltransferase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,090,0490GC 7.9% 144.3 / 2.1 48G296G (GGC→GGG) ‡astA‑IIarginine N‑succinyltransferase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (22/22);  new base C (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

TCTGTTGCCAGACCTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGGCCGCCGTCGAAGATGTCGATG  >  NC_002947/5089903‑5090187
                                                                                                                                                  |                                                                                                                                          
tCTGTTGCCAGACCTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGcc                                                                                                                                          >  6:249682/1‑149 (MQ=255)
tCTGTTGCCAGACCTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGcc                                                                                                                                          <  2:114611/149‑1 (MQ=255)
    ttGCCAGACCTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGtt                                                                                                                                       <  1:86398/148‑1 (MQ=255)
         aGACCTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACGGCCTGTTGcagga                                                                                                                                 <  2:65816/149‑4 (MQ=255)
            ccTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAg                                                                                                                              <  7:101139/149‑1 (MQ=255)
             cTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACAcc                                                                                                                                >  1:171250/1‑146 (MQ=255)
             cTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACAcc                                                                                                                                <  2:171250/146‑1 (MQ=255)
              tctaaCCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACGGCCTGTTGcagg                                                                                                                                  <  5:96973/140‑3 (MQ=255)
              tCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACGGCCTGTTGcagg                                                                                                                                  >  6:96973/1‑141 (MQ=255)
                  aCCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACgg                                                                                                                        <  3:150966/149‑1 (MQ=255)
                   ccGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACggg                                                                                                                       >  6:138627/1‑149 (MQ=255)
                    cgcgACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGgc                                                                                                                      >  4:228740/1‑149 (MQ=255)
                    cgcgACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGgc                                                                                                                      >  6:25310/1‑149 (MQ=255)
                           aGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGcgcccgcc                                                                                                               <  2:215509/149‑1 (MQ=255)
                             gcgcACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCtt                                                                                                             >  1:137135/1‑149 (MQ=255)
                                 aCGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGAc                                                                                                         >  7:7991/1‑149 (MQ=255)
                                     tGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCggg                                                                                                     >  7:100538/1‑149 (MQ=255)
                                       gcgcCCTCACCCACGCACAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCCACACCGCGCGGTAGTCGTGCAACAGGCCGTTGCACACCAGGGACGGGCGCCCGCCTTTGACCGGGGc                                                                                                   <  7:217104/149‑1 (MQ=255)
                                           ccTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCctct                                                                                               >  5:109869/1‑149 (MQ=255)
                                             tCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTc                                                                                             >  4:195428/1‑149 (MQ=255)
                                                 ccACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACCCCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATc                                                                                         >  1:45544/1‑149 (MQ=255)
                                                 ccACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTg                                                                                                                                      <  6:32267/104‑1 (MQ=255)
                                                 ccACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTg                                                                                                                                      >  5:32267/1‑104 (MQ=255)
                                                 ccACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATc                                                                                         >  1:38787/1‑149 (MQ=255)
                                                 ccACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATc                                                                                         <  2:45544/149‑1 (MQ=255)
                                                    cGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCggg                                                                                                     >  8:97697/1‑134 (MQ=255)
                                                    cGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCggg                                                                                                     <  7:97697/134‑1 (MQ=255)
                                                      cccAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGTTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGAt                                                                                          <  5:79435/143‑1 (MQ=255)
                                                      cccAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGAt                                                                                          >  6:79435/1‑143 (MQ=255)
                                                          gcgcGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCCTGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCATGTACGGTCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGc                                                                                <  8:253464/149‑1 (MQ=255)
                                                                         ccACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTgg                                                                 >  1:223703/1‑149 (MQ=255)
                                                                          cACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTggg                                                                <  5:25310/149‑1 (MQ=255)
                                                                          cACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTggg                                                                <  8:100538/149‑1 (MQ=255)
                                                                                   gCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGc                                                       <  2:137135/149‑1 (MQ=255)
                                                                                              tGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCagag                                            >  7:43502/1‑149 (MQ=255)
                                                                                                     cACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGCGCGGATGCCAGAGGTgcgcg                                     >  2:104290/1‑149 (MQ=255)
                                                                                                        ccAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCAt                                  <  6:109869/149‑1 (MQ=255)
                                                                                                                 gtccagCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCg                         >  7:54472/1‑149 (MQ=255)
                                                                                                                  tccagCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCgg                        <  2:38787/149‑1 (MQ=255)
                                                                                                                     agCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGgcc                     <  3:228740/149‑1 (MQ=255)
                                                                                                                      gCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGgccg                    >  4:110751/1‑149 (MQ=255)
                                                                                                                             cgcgcgGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGGCCGCCGTCGa             <  8:54472/149‑1 (MQ=255)
                                                                                                                               cgcgGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGGCCGCCGTCGAAg           <  1:108107/149‑1 (MQ=255)
                                                                                                                               cgcgGTAGTCCTGCAACAGGCCGGTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCGCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGGCCGCCGTCGAAg           <  5:138627/149‑1 (MQ=255)
                                                                                                                                      ggtgtGCAACGGCCTGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGGCCGCCGTCGAAGATGTCGa    >  8:164122/5‑149 (MQ=255)
                                                                                                                                         cTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGGCCGCCGTCGAAGATGGCGATg  >  8:243774/1‑148 (MQ=255)
                                                                                                                                          tGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCagag                                            <  5:256875/105‑1 (MQ=255)
                                                                                                                                          tGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCagag                                            >  6:256875/1‑105 (MQ=255)
                                                                                                                                                  |                                                                                                                                          
TCTGTTGCCAGACCTCAAACCGCGACCAGGCGCACGCTGGCGCCCTCACCCACGCCCAGCGCGTCGGCTGCTGCCACGCTCAGGCTGACCGGCTTGCCAGGCACCCAGTCCAGGTCCAGCAACACCGCGCGGTAGTCCTGCAACAGGCCGTTGCACACCAGGTACGGGCGCCCGCCTTTGACCGGGGCCTCTTCGATCTTCACCGGCACCACCCGGCTCTGGGCGATCGAGCGGATGCCAGAGGTGCGCGCATGCAGGGTCGGGCCGCCGTCGAAGATGTCGATG  >  NC_002947/5089903‑5090187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑