Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,105,065 A→T 8.2% intergenic (+88/+26) yhhS → / ← ydiJ carbohydrate efflux transporter/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,105,0650AT 8.2% 122.6 / 3.2 49intergenic (+88/+26)yhhS/ydiJcarbohydrate efflux transporter/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (25/20);  new base T (2/2);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

AGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGTTGCCAGCGGCTCCCCGTCCTTGTCC  >  NC_002947/5104921‑5105208
                                                                                                                                                |                                                                                                                                               
aGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAag                                                                                                                                              >  8:142687/1‑148 (MQ=255)
  ccTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAagcag                                                                                                                                           >  4:133648/1‑149 (MQ=255)
   ctctATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAagcagc                                                                                                                                          >  7:27209/1‑149 (MQ=255)
    tctATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGACGGGCTGCAAAGCAGcc                                                                                                                                         >  2:56662/1‑149 (MQ=255)
        tttGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCaaa                                                                                                                                               >  4:53903/1‑139 (MQ=255)
        tttGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCaaa                                                                                                                                               <  3:53903/139‑1 (MQ=255)
        tttGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCTTTGCAGccctt                                                                                                                                      <  5:162250/148‑3 (MQ=255)
        tttGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCTTTGCAGccctt                                                                                                                                      >  6:162250/1‑146 (MQ=255)
            tACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGGAGGAGCGGGTTCACCCGCGAAGGGCTGCTTTGCAGcccttcgcgg                                                                                                                                 <  6:159931/149‑8 (MQ=39)
                gCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGCAGGGCTGCAAAGCAGCCCCCAAAGGCCCt                                                                                                                             >  4:75328/1‑149 (MQ=255)
                    aGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAAc                                                                                                                         <  8:27209/149‑1 (MQ=255)
                         gTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAg                                                                                                                    >  1:261939/1‑149 (MQ=255)
                          tAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCACAGCAGCCCCCAAAGGCCAGTCACCTCAgc                                                                                                                   >  2:173357/1‑149 (MQ=38)
                             ggacagggacCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAgc                                                                                                                   >  6:173204/1‑146 (MQ=255)
                             ggacagggacCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAgc                                                                                                                   <  5:173204/146‑1 (MQ=255)
                                     acCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCCGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTAc                                                                                                        >  3:212436/1‑149 (MQ=255)
                                        gTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTg                                                                                                      >  6:98056/1‑148 (MQ=255)
                                               aGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAAcacca                                                                                              >  6:116666/1‑149 (MQ=255)
                                                gAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGCTCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCCGCCCCCAAAGGCCCTGAACCTCAGCCCGGAGCGTACGGcaccaccac                                                                                             >  4:126841/1‑149 (MQ=38)
                                                   gCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCACGCCCCCCTGGCGGGCACCATCCCCCCTAGGAGCGGGAGGACCCGCGAAGGGCAGCAAAGCCGCCCCCAAAGGCCCGGAACCTAAGCGCAGCCCGTACACAAACACCCCCt                                                                                           >  8:169839/1‑148 (MQ=255)
                                                     cTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCaa                                                                                        >  2:176364/1‑149 (MQ=255)
                                                        aaaGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACGCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCg                                                                                     <  7:169839/149‑1 (MQ=255)
                                                           gCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGt                                                                                  <  1:173357/149‑1 (MQ=255)
                                                                  ccGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGc                                                                             <  1:176364/147‑1 (MQ=255)
                                                                  ccGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCa                                                                            <  6:82872/148‑1 (MQ=255)
                                                                            cgcgGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTc                                                                  <  7:142687/148‑1 (MQ=255)
                                                                               gggTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCACAGCAGCCCCCCAAGGCCCGGCACCTCAGCGCTGAGCGGACGGCACCACCACCGCAAGCGGGTGACGCATCGTCCGCTCGGt                                                               >  6:121803/1‑148 (MQ=37)
                                                                                      cccGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCGCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCt                                                       >  6:243376/1‑149 (MQ=255)
                                                                                       ccGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCtt                                                      <  3:126841/149‑1 (MQ=255)
                                                                                        cGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTg                                                     <  2:261939/149‑1 (MQ=255)
                                                                                          ccccccTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGAc                                                   <  5:116666/149‑1 (MQ=255)
                                                                                          ccccccTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGAc                                                   >  7:183815/1‑149 (MQ=255)
                                                                                           cccccTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGAcc                                                  <  5:264084/149‑1 (MQ=255)
                                                                                             cccTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTg                                                >  7:241953/1‑149 (MQ=255)
                                                                                                    gTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACgg                                         >  5:34085/1‑149 (MQ=255)
                                                                                                         cGATCCGCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGTCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGa                                    <  1:243696/149‑1 (MQ=255)
                                                                                                          gATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGaa                                   <  5:121803/149‑1 (MQ=255)
                                                                                                            tCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATa                                 <  8:183815/149‑1 (MQ=255)
                                                                                                               cccGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAAcc                              >  7:86399/1‑149 (MQ=255)
                                                                                                                ccGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCg                             <  6:34085/149‑1 (MQ=255)
                                                                                                                  gTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTGCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGt                           <  1:56662/149‑1 (MQ=255)
                                                                                                                          gggTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCg                             <  4:145338/139‑1 (MQ=255)
                                                                                                                          gggTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCg                             >  3:145338/1‑139 (MQ=255)
                                                                                                                           ggTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGTTGCCAGCgg                  <  8:86399/149‑1 (MQ=255)
                                                                                                                                aCCCGCGAAGGGCTGCAAAGCCGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCAAGCGCTTGACCTGGCTACGGCACGAAAAACCGGTTGCCAGCGGCTccc             >  8:166066/1‑149 (MQ=255)
                                                                                                                                  ccGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGTTGCCAGCGGCTCCCCg           >  2:113545/1‑149 (MQ=255)
                                                                                                                                   cgcgAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGTTGCCAGCGGCTCCCCGt          >  6:273394/1‑149 (MQ=255)
                                                                                                                                        ggggCTGCTTTGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGTTGCCAGCGGCTCCCCGTCCTTg     >  8:222400/2‑149 (MQ=255)
                                                                                                                                          ggCTGCAAAGCAGCCCCGAATGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTGCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCTGTTGCCAGCGGCTCCCCGTCGTTGt    <  7:166066/148‑1 (MQ=255)
                                                                                                                                           gCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGTTGCCAGCGGCTCCCCGTCCTTGTcc  <  4:212436/149‑1 (MQ=255)
                                                                                                                                            cTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGTTGCCAGCGGCTCCCCGTCCTTGTcc  >  8:218133/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
AGCCTCTATTTGTACCGCCAAGGGCGTAGGGACAGGGACCGTACAACAGAAGCCTGAAAGCCGCTGCCGGCCTCTTCGCGGGTAACCCCGCCCCCCTGGCGTGCACGATCCCCCGTAGGAGCGGGTTCACCCGCGAAGGGCTGCAAAGCAGCCCCCAAAGGCCCTGAACCTCAGCGCTGAGCGTACTGCAACACCACCTCAAGCGGGTGACGCATCTTCCGCTCGGTCATGCGCTTGACCTGGCTACGGCACGAATAACCGGTTGCCAGCGGCTCCCCGTCCTTGTCC  >  NC_002947/5104921‑5105208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑