| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,586,030 | 2 bp→TC | 100% | intergenic (+113/+101) | PP_4061 → / ← PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,586,030 | 0 | C | T | 94.8% | 51.6 / ‑4.8 | 19 | intergenic (+113/+102) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (3/15); total (4/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_002947 | 4,586,031 | 0 | T | C | 95.2% | 53.5 / ‑5.6 | 19 | intergenic (+114/+101) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (3/15); minor base G (1/0); total (4/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TCTGC‑GGTAAACCCGCTCCTACCAGGGA‑C‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGA > NC_002947/4585887‑4586112 || gccgAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGC‑gg < 2:79520/149‑1 (MQ=255) aCCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTa < 4:167949/149‑1 (MQ=255) ccGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGC‑gg < 6:154707/133‑1 (MQ=255) ccGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGC‑gg > 5:154707/1‑133 (MQ=255) ggTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCa < 6:117198/149‑1 (MQ=255) gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAggg > 8:190753/1‑149 (MQ=255) aGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGATGATCTTGCATTGCTCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGcc < 4:248827/148‑1 (MQ=38) aGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCg < 6:170166/149‑1 (MQ=39) aGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCAATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCC‑GGTAAACCCGCTCCTACCAGGGACC‑GTGCCg > 5:170166/1‑149 (MQ=39) gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTT‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGc < 1:208872/149‑1 (MQ=39) gTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGccc < 6:96800/149‑1 (MQ=39) tGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGcccc < 5:196042/149‑1 (MQ=39) aTCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAgg < 1:132233/146‑1 (MQ=39) aTCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAgg > 2:132233/1‑146 (MQ=39) cgccgcTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCCGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGtt < 7:18928/149‑1 (MQ=38) cgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGc < 2:38390/144‑1 (MQ=38) gctgctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGCTCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGACC‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGttt < 7:236332/149‑1 (MQ=38) aaaGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAGGGG‑CAGCGCC‑GCATTCAAGGCCGGCACGGTCCCTGGTAGGAGCGGGTTTACCCGCGAaga < 7:190753/148‑1 (MQ=14) ttGGCAATGTGAAGATCTTGCATTGATCTGGCCGGCCTC‑TTCGCGGGTTAACCCGCTCCTTCCAGGGACC‑GTGCCGTCGTTGAATG‑CGGCGCTGCCGCTg < 3:57843/100‑1 (MQ=255) || GCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TCTGC‑GGTAAACCCGCTCCTACCAGGGA‑C‑GTGCCGGCCTTGAATG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGA > NC_002947/4585887‑4586112 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |