Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 336,125 +T coding (210/237 nt) PP_0278 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947336,1241.T97.3% 119.4 / NA 37D70V (GAT→GTT) PP_0278hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base T (12/24);  minor base G (0/1);  total (12/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGA  >  NC_002947/335980‑336265
                                                                                                                                                 |                                                                                                                                              
aCCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑tta                                                                                                                                             >  6:119383/1‑146 (MQ=255)
 cccATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑tta                                                                                                                                             <  4:29768/147‑3 (MQ=255)
 cccATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑tta                                                                                                                                             <  2:129927/147‑3 (MQ=255)
   cATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttacct                                                                                                                                          <  6:27894/148‑6 (MQ=255)
   cATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttacct                                                                                                                                          <  2:25321/148‑6 (MQ=255)
    aTCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCtg                                                                                                                                          >  5:104327/1‑148 (MQ=255)
                  tACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCaa                                                                                                                            >  7:42675/1‑148 (MQ=255)
                  tACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCaa                                                                                                                            <  5:119383/148‑1 (MQ=255)
                     gcgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAgg                                                                                                                          <  4:115809/147‑1 (MQ=255)
                       gTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGgcgc                                                                                                                       >  7:130082/1‑148 (MQ=255)
                       gTAGAGCCCGGCGTCCCCGGCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGgcgc                                                                                                                       <  5:158943/148‑1 (MQ=255)
                           agCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCtgat                                                                                                                   <  8:130082/148‑1 (MQ=255)
                               cGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAAc                                                                                                               <  7:145574/148‑1 (MQ=255)
                               cGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAAc                                                                                                               <  8:148246/148‑1 (MQ=255)
                                       ccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGCCGCCCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGatat                                                                                                          >  3:28729/1‑145 (MQ=255)
                                           cGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATc                                                                                                   <  2:169482/148‑1 (MQ=255)
                                                cgGCCTACGAGCATGTCTCGATCCTGATTGGCTACATCAAGCACCGGGTGCGCGAGGGCGATATTGAGGACGACCATAAATTCCTGGGGGCAGCCGAGTACCTGTGCGACATGGCCAAGCCGCTGATGAACGATATCGAGATCGCCaa                                                                                              <  3:93551/148‑1 (MQ=255)
                                                     tACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTa                                                                                         <  1:88206/148‑1 (MQ=255)
                                                          gCATGTCTCGATACTGATGGGCTACATCAAGCACCTGGTGCGCGAGTGCGATATGGAGGACTACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGTGTAAGgcg                                                                                    <  8:131885/148‑1 (MQ=255)
                                                                     tCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCAt                                                                          >  2:154768/1‑147 (MQ=255)
                                                                        tGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTCCCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGAc                                                                      >  8:75340/1‑148 (MQ=255)
                                                                         gATGGTCTACATCAAGCACCTGGTGCGCGAGGTCGATATGGAGGACGACCATAAATTCCTGGGGTCAGCCGATTACCTCTGCGACATTGCCAAGGAGCTGATTAACGATATCGAGATCGCCAAGGGTACTGCGCATTAGGCATTGa                                                                       >  5:62936/1‑146 (MQ=255)
                                                                               cTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCa                                                               >  3:155289/1‑148 (MQ=255)
                                                                                tACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCACGGGTAAGGCGCATTAGGCATTGACGCGGTCAt                                                              <  8:51741/148‑1 (MQ=255)
                                                                                   aTCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAttt                                                            <  3:157399/147‑1 (MQ=255)
                                                                                   aTCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtg                                                           >  8:74007/1‑148 (MQ=255)
                                                                                      aaGCACCTTGTGCGCTAGGGAGATATTGATGACGACCATAAATTCCTGGGGGAAGCCGATTAACTGTGCGACATGGCCAAGGCTCTTATGAACTATATCGAGATCGCCAATGGTAAGGCTCATTAGTCATTGACGCTGTCATTtgt                                                          <  5:6607/146‑1 (MQ=255)
                                                                                       aGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTgg                                                        <  3:24919/147‑1 (MQ=255)
                                                                                                   gcgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGc                                           <  3:28249/147‑1 (MQ=255)
                                                                                                       gggCGATATGGAGGACGACCATACATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTcgc                                      <  1:163262/148‑1 (MQ=255)
                                                                                                       gggCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTcgc                                      <  2:135304/148‑1 (MQ=255)
                                                                                                             tatGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTCGCGAATgg                                <  3:29996/148‑1 (MQ=255)
                                                                                                              atGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTCGCGAATggg                               <  6:41637/148‑1 (MQ=255)
                                                                                                                ggaggaCGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGACCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTCGCGAATGGGc                              <  8:63159/147‑1 (MQ=255)
                                                                                                                     acgacCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTCGCGAATGGGCTGCaaa                        <  4:173037/148‑1 (MQ=255)
                                                                                                                         ccATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGGC‑CTTCGGCGGGACTGGGCTGGAAaccac                    >  3:142769/1‑147 (MQ=255)
                                                                                                                          cATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTCGCGAATGGGCTGCAAagcagc                   <  6:170544/148‑1 (MQ=255)
                                                                                                                                ttCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAg                                                                                             >  3:66285/1‑69 (MQ=255)
                                                                                                                                 tCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAgg                                                                                            <  4:66285/69‑1 (MQ=255)
                                                                                                                                         gCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGCGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTCGCGAATGGGCGGCAAAGCAGCCCCGGCGATTTCaga    <  8:3538/148‑1 (MQ=255)
                                                                                                                                         gCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCaga    <  7:142626/148‑1 (MQ=255)
                                                                                                                                           aGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCATTCCGT‑GCGCATGGGCTGCCAAGCACCCCCCGCGCATTTAgaga  >  6:40698/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
ACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCC‑TTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGAGA  >  NC_002947/335980‑336265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑