| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,126,647 | +C | intergenic (‑101/+48) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 79.2% | 62.0 / 12.8 | 24 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (5/0); new base C (6/13); total (11/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AA‑A‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCA > NC_002947/1126498‑1126784 | tGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATTCGGAGGTATATCCACCATCGCCATAGCGGG‑T‑aa‑a > 7:112284/1‑148 (MQ=255)tGGAGGGGCCGATGCCGATCTTGAACAGGGCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑aa‑a < 7:105859/148‑1 (MQ=255) ggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AA‑A‑cccgct < 2:165073/148‑5 (MQ=255) gccgatgccgatCTTGAACCGGTCGAAGACGTTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AA‑A‑cccgctc < 7:1427/148‑6 (MQ=255) aaCAGGTCGAAGACGCTCAGGGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AA‑ACCCGCTCCTACACAGAGCCCGc < 2:31871/147‑1 (MQ=255) ccgacgaCGCGCAGTGACATGGGGACCTCCGGCCAGGTGGGGGGGTGGACATGCAGCCCTGGAGGCGCGGG‑TTTACCCGCGAAGGCGGAGGTAAAGCCACCACCGCATTCGCGGG‑T‑AA‑ACCCGCTCCTACACAGAGCCCGCCACCCaa < 3:16826/147‑1 (MQ=21) gAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTTACCCCGAAAGGGGGGGTTTAACCCCCAAAGCAGTAGCGGG‑TAAA‑A‑CCCCGCCGACACAGAGCCCCCCCCCCCAg > 4:74653/1‑147 (MQ=255) gACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAg < 3:132305/148‑1 (MQ=255) aCGCGCAGTGACATGTGGACCTCCTGCCAGGTTGTGGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAgg < 7:160887/148‑1 (MQ=255) tggggACCTCCTGCCAGGTTGTGGGGGGCACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGc < 3:62640/145‑1 (MQ=37) ggACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTAt < 6:69813/148‑1 (MQ=255) cAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTcc < 3:146609/146‑1 (MQ=255) gTGGACATTGAGCCCTGTAGGCGCGGG‑TTTTACCCCGAATGGGGGGGTATAGCCACCAACGCATTAGCGGG‑T‑AA‑ACCCCCGCCGACACACAGCCCCCCCCCCCAGCCAGGGGGCGGGGGGGGCGATAAGGCgggcgga > 2:121347/1‑132 (MQ=255) tGGACATTGAGCCCTGTAGGCGCGGGTTTTAACCCCCATGGCGGGGGT‑TAGCCACCCACGCAATAGCGGG‑TGAA‑C‑CCCCGCCGACACACACCCCCCCCACCCACCCCGGTGGGGGGGCGGGGTATCACGCGGCCTGGTAGccggcgca > 8:129290/1‑141 (MQ=255) gggACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGAt < 1:121347/137‑1 (MQ=255) aGGCGGGGG‑TTTACCCGCGAATGGGGCGGGAAACCCCCCCACGCAGTCGCGGG‑T‑AA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGca < 3:128993/146‑1 (MQ=255) aGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGGCCTGATAGCTCTCGATCGACGGGCAGGCGcaca < 8:112284/148‑1 (MQ=255) gcgGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AACC‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCCCACCAg > 1:40850/1‑148 (MQ=255) g‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGGTT‑AA‑C‑CCGCTGCTACCCAGAGCCCGCCACCCACGCCAGGTGGCGGGGAGGGCTCTCAGGCGTCCTGATCGCTCTCGATCGCCGGGCAGGCGCACCCCAGGTTg > 5:703/1‑148 (MQ=255) ccGCGAATGCGGAGGTATAGCCCCCATCGCATTCGCGGG‑T‑AA‑ACCCGCTCCTCCACAGAGCCCGCCCCCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGCCGGGCAGGCGCACACCAGGTTGCGGTCg > 4:41298/1‑148 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCg > 6:103092/1‑148 (MQ=255) cgcgAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AACC‑CCGCTCCTACACAGAGCCCGCCCCCCACGCCAGGGGGCGGGGAGGGCTATCAGGCGTCCTGCTAGCTCTCGAGCGACGGGCAGGCGCCCCCCAGGTTGCGGgcgc > 5:127406/1‑148 (MQ=255) atccccaccaTCCCATGCGCGGT‑T‑AA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGa < 6:20402/144‑1 (MQ=255) ccaccaTCGCATTCGCGGG‑T‑AA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGCCGGGCAGGCGCACACCACGTTGCGGTCGCCGAACACGTAGTCGACCCg > 2:91364/1‑148 (MQ=255) cATTCGCGGG‑T‑AA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACtgg > 3:46154/1‑148 (MQ=255) ttCGCGGG‑T‑AA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGGCGCCGAACACGTTGTCGACCCGGCCGACcggcg < 8:85112/148‑1 (MQ=255) gcgGG‑T‑AA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc < 7:148138/147‑1 (MQ=255) ggg‑T‑AA‑ACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGCCCGTCGGCCa > 8:58148/1‑147 (MQ=255) | TGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGCGGG‑T‑AA‑A‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCA > NC_002947/1126498‑1126784 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |