Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,791,9160CA29.0% 42.2 / 18.2 31A642S (GCT→TCT) pvdInon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (11/11);  new base A (9/0);  total (20/11)
Fisher's exact test for biased strand distribution p-value = 1.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTCACGCAGCATGAAT  >  NC_002947/4791771‑4792061
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gcAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCa                                                                                                                                                 <  2:71141/148‑1 (MQ=255)
gcAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCa                                                                                                                                                 <  4:105311/148‑1 (MQ=255)
  aGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGACAgc                                                                                                                                               >  4:10469/1‑147 (MQ=255)
              cATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGCTCACATAAGCCAGGTTGCCGGAGTGa                                                                                                                                   >  5:74989/1‑148 (MQ=255)
              cATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCACGTTCCCGTAGTg                                                                                                                                    >  2:84637/1‑147 (MQ=255)
                             cAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGCCCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGcccc                                                                                                                    >  1:13520/1‑148 (MQ=255)
                              aGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGa                                                                                                                                       >  5:9072/1‑128 (MQ=255)
                               gATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAg                                                                                                                                      <  6:9072/128‑1 (MQ=255)
                                      aaGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGCCCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGAGCACCCCTGCCTCg                                                                                                           >  3:27690/1‑148 (MQ=255)
                                            aaTCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGACAGGTTAACATATTAAAAATCAACGGGCACCCCTGCCTCGCCACCt                                                                                                     >  2:81961/1‑148 (MQ=255)
                                                   tCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGACACCTTAACTTAAAAAACAAACACGGGCACCACTGCCTCGCCACCTGCcgcg                                                                                               >  4:124460/1‑147 (MQ=255)
                                                         aaGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAgcg                                                                                        >  7:156491/1‑148 (MQ=255)
                                                                              aGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGACACCTTAACATAAAAAAAACCCACGGACACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGa                                                                   >  4:72434/1‑148 (MQ=255)
                                                                                 gcCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGACACCTTAACATAAAAAAACGAAACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATgg                                                                >  2:89975/1‑148 (MQ=255)
                                                                                           acacCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGGTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAgg                                                      <  5:95660/148‑1 (MQ=255)
                                                                                               cgcgACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGACACCTTAACATAAAAAAAAAAAACGGGCAACCCTGCCTCGCCACCTGCCGCGTTCAGTGCCAGGCACTGTACGCCCCCGATTGCCGGGCCAGGCTCg                                                  >  2:99425/1‑148 (MQ=255)
                                                                                                   aCCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGACACCTTAACATAAAAAAAAAAAACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCt                                              >  4:83059/1‑148 (MQ=255)
                                                                                                       ccTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCgg                                                            >  3:15954/1‑130 (MQ=255)
                                                                                                        cTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCggg                                                           <  4:15954/130‑1 (MQ=255)
                                                                                                            ggCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCt                                       <  1:102512/146‑1 (MQ=255)
                                                                                                             gCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGa                                     <  1:97716/147‑1 (MQ=255)
                                                                                                             gCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGACACCTTAACATAAAAAAAAAACACAGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCCCTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGa                                     >  4:3726/1‑147 (MQ=255)
                                                                                                                          gCCCGAGGTGTAGATCACATAAGACACCTTAACATAAAAAAAAAAAACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATc                       >  2:156705/1‑148 (MQ=255)
                                                                                                                             cGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGTCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCtgt                    <  3:113101/148‑1 (MQ=255)
                                                                                                                                 gtgtAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGt                <  4:168454/148‑1 (MQ=255)
                                                                                                                                  tgtAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGCTGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTc               >  5:112048/1‑148 (MQ=255)
                                                                                                                                       aTCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTCACgca          >  5:108242/1‑148 (MQ=255)
                                                                                                                                           caTAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTCACGCAGCAt      <  6:96162/148‑1 (MQ=255)
                                                                                                                                            aTAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTCACGCAGCAt      >  4:135710/1‑147 (MQ=255)
                                                                                                                                            aTAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTCACGCAGCAt      >  6:127698/1‑147 (MQ=255)
                                                                                                                                                gCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTCACGCAGCATGAAt  <  8:156491/147‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GCAGGCGTGGAGACCATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTCACGCAGCATGAAT  >  NC_002947/4791771‑4792061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑