Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,791,9270GT31.0% 38.9 / 13.1 29S638Y (TCC→TAC) pvdInon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (11/9);  new base T (9/0);  total (20/9)
Fisher's exact test for biased strand distribution p-value = 2.70e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTCACGCAGCATGAATGCCATTCGCTGCT  >  NC_002947/4791785‑4792074
                                                                                                                                              |                                                                                                                                                   
cATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGCTCACATAAGCCAGGTTGCCGGAGTGa                                                                                                                                                >  5:74989/1‑148 (MQ=255)
cATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCACGTTCCCGTAGTg                                                                                                                                                 >  2:84637/1‑147 (MQ=255)
               cAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGCCCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGcccc                                                                                                                                 >  1:13520/1‑148 (MQ=255)
                aGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGa                                                                                                                                                    >  5:9072/1‑128 (MQ=255)
                 gATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAg                                                                                                                                                   <  6:9072/128‑1 (MQ=255)
                        aaGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGCCCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGAGCACCCCTGCCTCg                                                                                                                        >  3:27690/1‑148 (MQ=255)
                              aaTCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGACAGGTTAACATATTAAAAATCAACGGGCACCCCTGCCTCGCCACCt                                                                                                                  >  2:81961/1‑148 (MQ=255)
                                     tCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGACACCTTAACTTAAAAAACAAACACGGGCACCACTGCCTCGCCACCTGCcgcg                                                                                                            >  4:124460/1‑147 (MQ=255)
                                           aaGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAgcg                                                                                                     >  7:156491/1‑148 (MQ=255)
                                                                aGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGACACCTTAACATAAAAAAAACCCACGGACACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGa                                                                                >  4:72434/1‑148 (MQ=255)
                                                                   gcCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGACACCTTAACATAAAAAAACGAAACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATgg                                                                             >  2:89975/1‑148 (MQ=255)
                                                                             acacCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGGTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAgg                                                                   <  5:95660/148‑1 (MQ=255)
                                                                                 cgcgACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGACACCTTAACATAAAAAAAAAAAACGGGCAACCCTGCCTCGCCACCTGCCGCGTTCAGTGCCAGGCACTGTACGCCCCCGATTGCCGGGCCAGGCTCg                                                               >  2:99425/1‑148 (MQ=255)
                                                                                     aCCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGACACCTTAACATAAAAAAAAAAAACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCt                                                           >  4:83059/1‑148 (MQ=255)
                                                                                         ccTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCgg                                                                         >  3:15954/1‑130 (MQ=255)
                                                                                          cTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCggg                                                                        <  4:15954/130‑1 (MQ=255)
                                                                                              ggCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCt                                                    <  1:102512/146‑1 (MQ=255)
                                                                                               gCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGa                                                  <  1:97716/147‑1 (MQ=255)
                                                                                               gCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGACACCTTAACATAAAAAAAAAACACAGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCCCTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGa                                                  >  4:3726/1‑147 (MQ=255)
                                                                                                            gCCCGAGGTGTAGATCACATAAGACACCTTAACATAAAAAAAAAAAACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATc                                    >  2:156705/1‑148 (MQ=255)
                                                                                                               cGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGTCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCtgt                                 <  3:113101/148‑1 (MQ=255)
                                                                                                                   gtgtAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGt                             <  4:168454/148‑1 (MQ=255)
                                                                                                                    tgtAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGCTGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTc                            >  5:112048/1‑148 (MQ=255)
                                                                                                                         aTCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTCACgca                       >  5:108242/1‑148 (MQ=255)
                                                                                                                             caTAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTCACGCAGCAt                   <  6:96162/148‑1 (MQ=255)
                                                                                                                              aTAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTCACGCAGCAt                   >  6:127698/1‑147 (MQ=255)
                                                                                                                              aTAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTCACGCAGCAt                   >  4:135710/1‑147 (MQ=255)
                                                                                                                                  gCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTCACGCAGCATGAAt               <  8:156491/147‑1 (MQ=255)
                                                                                                                                          gCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCCGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTCACGCAGCATGAATGCCATTCgc      >  8:122198/1‑148 (MQ=255)
                                                                                                                                              gAGTGAACCGCCACGAGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTCACGCAGCATGAATGCCATTCgctgct  <  4:27690/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
CATGGCGAAGTGTTCCAGATCGCCAAGGGCAATCCGCTCGGACAAGCCTTCAAGGTAATTGGCCAGCGCCCCATGAGACACCGCGACCCCCTTGGGCATACCGGTGGTGCCCGAGGTGTAGATCACATAAGCCAGGTTGCCGGAGTGAACCGCCACGGGCACCCCTGCCTCGCCACCTGCCGCGTTCAGCGCCAGGCACTGTACGCCCCCGATGGCCGGGCCAGGCTCGTCCTCGGCCCTGAGCAAGACCTTGATCTGTGCGTCACGCAGCATGAATGCCATTCGCTGCT  >  NC_002947/4791785‑4792074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑