Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947195,6340CT37.8% 39.5 / 40.8 47T380T (ACC→ACT) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (14/14);  new base T (11/6);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 3.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAG  >  NC_002947/195495‑195778
                                                                                                                                           |                                                                                                                                                
aGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGACCGCCACCGGCGGTAAATTCGATCACCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGAc                                                                                                                                          <  6:25670/148‑1 (MQ=11)
aGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGAc                                                                                                                                          >  6:42632/1‑148 (MQ=39)
 gACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGAcc                                                                                                                                         >  7:26836/1‑148 (MQ=39)
    cccGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGAAATTTAGCGCAACTGCCCCCGAAGCAGACCCCGGATCAGCCCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTg                                                                                                                                      >  2:94971/1‑148 (MQ=11)
    cccGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTg                                                                                                                                      >  5:83821/1‑148 (MQ=39)
    cccGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATACGCCCCCGGCTCAGACCACGATCAACGACTCGGTCGCCACCACACCCGCGCCCCTg                                                                                                                                      >  1:86231/1‑148 (MQ=14)
                 gTCTACAACAACGGTTCGACCGTCCGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGt                                                                                                                         >  4:72214/1‑148 (MQ=12)
                       aacaacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCgg                                                                                                                      <  3:84149/145‑1 (MQ=37)
                          aacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCCGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCAcc                                                                                                                 >  4:21043/1‑147 (MQ=37)
                                    ccGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt                                                                                                      <  5:157505/148‑1 (MQ=17)
                                          gCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGCGCCCGTCGGTCACCGAAGGCGGCGTGATCAc                                                                                                >  8:161280/1‑148 (MQ=38)
                                          gCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc                                                                                                <  5:42632/148‑1 (MQ=38)
                                                   tCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGAcaccacc                                                                                                                                                  <  3:31745/89‑1 (MQ=18)
                                                   tCGAGAAAGCCACATGCGGTAATTTCGAGCAACTTACACCGAATACTACCCCAACTCATAAAACTATAAAATACAAGGTCGACAACACTAAATCTACAATGACGACGAACAAGTCGGTCAAATAAGGCGGCGTGATCACTTACACCg                                                                                        >  6:67559/1‑147 (MQ=2)
                                                       gaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAcc                                                                                   >  8:27156/1‑148 (MQ=17)
                                                           gCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGa                                                                               <  1:44239/148‑1 (MQ=39)
                                                           gCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACATACACCGTGACCCTGa                                                                               >  5:34155/1‑148 (MQ=37)
                                                           gCCACCGGCGGTAATTTCCAGCAACTCACCCCGAATCCGACCCCGGCTCAGACCACGATCCACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGGCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGa                                                                               <  2:89173/148‑1 (MQ=255)
                                                           gCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGa                                                                               <  6:30706/148‑1 (MQ=17)
                                                                aggcggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACGTACACCGTGACCCTGAGCAAc                                                                          >  3:68691/2‑148 (MQ=17)
                                                                  gcggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAAccc                                                                        >  4:83449/1‑148 (MQ=39)
                                                                    ggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTg                                                                      >  5:63151/1‑148 (MQ=39)
                                                                          ttCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGa                                                                >  5:151513/1‑148 (MQ=21)
                                                                               gCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCg                                                           <  1:121094/148‑1 (MQ=17)
                                                                                    tGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACAACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACATACACCGTGACCCTGAGCaa                                                                           >  4:135383/1‑127 (MQ=25)
                                                                                      aCCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCg                                                    >  3:82877/1‑148 (MQ=40)
                                                                                       ccccGAATCCGACCCCGGCTCAGTCCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGcc                                                            >  3:25879/1‑139 (MQ=21)
                                                                                        cccGAATCCGACCCCGGCTCAGTCCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCg                                                           <  4:25879/139‑1 (MQ=21)
                                                                                        cccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCCCCGCGACCCTGACGGCGAGCCCGTCCGTCACCGAAGGCGGCGGGCTCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGAACCCGTg                                                  >  3:111737/1‑148 (MQ=14)
                                                                                        cccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACGTACACCGTGACCCTGAGCAAACCTGCCCAGACGCCGGTAACAGTg                                                  >  4:56069/1‑148 (MQ=255)
                                                                                         ccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGa                                                 >  5:107769/1‑148 (MQ=12)
                                                                                           gAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAAc                                               <  4:82877/148‑1 (MQ=255)
                                                                                                cGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGGCACCGAAGGCGGCGTTATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTc                                          <  5:158621/148‑1 (MQ=255)
                                                                                                 gACCCCGGCTCAGACCACGATCAACGAATCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCGGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTc                                          >  5:111321/1‑147 (MQ=255)
                                                                                                     ccGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACg                                     <  3:72214/148‑1 (MQ=37)
                                                                                                              aCCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCATGAACCTGTCCAACGGCCAAACGa                            <  6:86010/148‑1 (MQ=255)
                                                                                                                cACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTACCCGTGAACCTGTCCAACGGCCAAACGAtt                          >  5:161704/1‑148 (MQ=255)
                                                                                                                cACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGAtt                          >  5:167089/1‑148 (MQ=255)
                                                                                                                   gATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTAcc                       <  8:26836/148‑1 (MQ=39)
                                                                                                                    aTCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCg                      <  6:151513/148‑1 (MQ=255)
                                                                                                                       aaCGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTa                                                                                             >  4:48808/1‑74 (MQ=34)
                                                                                                                        aCGACTCGGTCGAGCCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGa                  <  5:18119/148‑1 (MQ=255)
                                                                                                                        aCGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGtt                    >  6:9955/1‑146 (MQ=255)
                                                                                                                        aCGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTac                                                                                            <  3:48808/74‑1 (MQ=34)
                                                                                                                           aCTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGc               >  3:107877/1‑148 (MQ=39)
                                                                                                                             tCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg             <  8:114680/148‑1 (MQ=39)
                                                                                                                                   gACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTaaaa       <  6:53449/148‑1 (MQ=39)
                                                                                                                                    acacCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGa                                                                               <  3:146629/75‑1 (MQ=34)
                                                                                                                                        caccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAg  >  6:83376/1‑148 (MQ=39)
                                                                                                                                           caccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGt                     >  5:70788/1‑126 (MQ=40)
                                                                                                                                           |                                                                                                                                                
AGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAG  >  NC_002947/195495‑195778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑