Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,544,501	0	G	C	35.7%	2.7 / 11.5	14	intergenic (+60/+54)	mdfA/ampG	multidrug MFS transporter/muropeptide permease AmpG
Reads supporting (aligned to +/- strand):  ref base G (2/7);  new base C (3/2);  total (5/9)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTA  >  NC_002947/1544361‑1544645                                                                                                                                             |                                                                                                                                                 ggggggGCGGGGGCGTGGGCGCTGGGGCCGCTGTGGTTGGTGGTGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTa                                                                                                                                           <  3:74332/148‑1 (MQ=39)        gggggggggTGGGGCGGTGGCCGGTGTGGTTTGTGGTGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCgg                                                                                                                                    <  1:83364/147‑1 (MQ=17)                     ggtggggcggcTGGGGTTGGTGGGGGGGGGGCGGCGTGGGGACGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTGCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGaa                                                                                                                       <  3:72388/143‑1 (MQ=25)                                                             gAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCATCGCGGGTAAAACCGCGCCGACCCCCGGGCGCGCCGCGGTGTAGGCGCGGGGGTTCCCGCGCAGAGGCCAGCACCGGCCATACCACGCGTCCGGGgcgcgacg                                                                               >  6:130081/1‑144 (MQ=255)                                                                                       atgccgggggCCGGCCTCTGCGCGGGTAAACCCCCGCCAACACCGGCCCGCGCCGCTTTGTCGGCGCGGGTTAACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGc                                                     <  5:126311/142‑1 (MQ=255)                                                                                                             cgGGTAAACCCGCTCCTACACCGGGCCGCGCCGCTTTTTAGCAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGa                                <  8:4510/147‑1 (MQ=255)                                                                                                                    ccccGCGCCTCCACCGGCCCGCGCCGCTTGGTCGGCGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTg                        <  3:73353/147‑1 (MQ=38)                                                                                                                      ccGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCa                      >  6:163775/1‑148 (MQ=255)                                                                                                                        gCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCCCAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCCCTGCAgg                    >  2:107971/1‑148 (MQ=25)                                                                                                                        gCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg                    >  3:60773/1‑148 (MQ=37)                                                                                                                            cTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg                     >  1:120967/1‑143 (MQ=37)                                                                                                                             tACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg                    <  2:120967/143‑1 (MQ=37)                                                                                                                               ccccgggCCGCGCCGCTTTGTCGGCGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCCGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca             <  2:103145/144‑1 (MQ=255)                                                                                                                                acaGGGCCGCGCCGCTTTGTAGGAGCGGG‑TTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca             <  4:39579/146‑1 (MQ=255)                                                                                                                                          gccgcTTTGTCGGCGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTa  <  5:170333/148‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                 GGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATCAGCGTA  >  NC_002947/1544361‑1544645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑