Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,022,6330CT29.6% 34.1 / 16.4 27intergenic (‑328/+340)PP_3547/emrBNAD(P)‑binding oxidoreductase/multidrug transporter permease
Reads supporting (aligned to +/- strand):  ref base C (12/7);  new base T (4/4);  total (16/11)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCC  >  NC_002947/4022490‑4022775
                                                                                                                                               |                                                                                                                                              
cAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGTATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAGGTGGCGCATAGTCACCCTTcc                                                                                                                                            <  1:145008/148‑1 (MQ=255)
            cGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGCGTAGGTATTCATTCGCGCAGGGGGCGCATAGTCACCTTTCCGCCCTTAcggcg                                                                                                                                >  4:96590/1‑148 (MQ=255)
              cAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTAcggcgg                                                                                                                               <  2:87751/147‑1 (MQ=255)
              cAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTAcggcgg                                                                                                                               <  3:45995/147‑1 (MQ=255)
              cAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAGGTGGCGCATAGACACCCTTCCGCCCTTACGGCggg                                                                                                                              >  1:152408/1‑148 (MQ=255)
              cAAACGGACGGTTCGCTGAGGCAAAACAGACGAGTGCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGGGGGCATTCACATCGAGTAGGTATTCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTAcggcgg                                                                                                                               <  7:80714/147‑1 (MQ=255)
                    gacggTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGCGTAGGTATTCATTCGCGCAGGGGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTCACtt                                                                                                                        >  3:88521/1‑148 (MQ=255)
                       ggTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGCGTAGGTATTCATTCGCGCAGGGGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTCACttttt                                                                                                                     <  6:176079/148‑1 (MQ=255)
                       ggTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACttttt                                                                                                                     <  8:146246/148‑1 (MQ=255)
                            gCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCaa                                                                                                                >  6:132009/1‑148 (MQ=255)
                                 gTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGCGTAGGTATTCATTCGCGCAGGGGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAAcgc                                                                                                            <  7:7252/147‑1 (MQ=255)
                                  tCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGGATTCACATCGCGTAGGTATTCATTCGCGCAGGGGGCGCATAGTCAACTTTACGCCCTTAAGGCGTGTCAATTTTTGTCAAAcgcg                                                                                                           >  7:147215/1‑147 (MQ=14)
                                  tCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGCGTAGGTATTCATTCGCGCAGGGGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAAcgcg                                                                                                           >  5:130963/1‑147 (MQ=255)
                                  tCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGCGTAGGTATTCATTCGCGCAGGGGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGa                                                                                                          <  4:88521/148‑1 (MQ=255)
                                      aaCAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACaa                                                                                                       >  3:76871/1‑147 (MQ=255)
                                        cAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGCGTAGGTATTCATTCGCGCAGGGGGCGCATAGTCACCTTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACaaaaa                                                                                                    <  7:134029/148‑1 (MQ=255)
                                                                          ttcttgcttcttgGATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGcc                                                                  >  3:148146/1‑148 (MQ=39)
                                                                             ttgcttcttgGATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCt                                                               >  7:32231/1‑148 (MQ=39)
                                                                                    ttgGATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTAc                                                        >  5:9079/1‑148 (MQ=39)
                                                                                            gggCATTCACATCGAGTAGGTATTCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCggg                                                <  6:70928/148‑1 (MQ=39)
                                                                                               cATTCACATCGAGTAGGTATTCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGtt                                             >  7:83806/1‑148 (MQ=39)
                                                                                                      aTCGAGTAGGTATTCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTACCTCGc                                      >  5:106246/1‑148 (MQ=255)
                                                                                                              ggTATTCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCt                              >  3:96857/1‑148 (MQ=255)
                                                                                                                         aCGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGgga                   >  6:5517/1‑148 (MQ=40)
                                                                                                                         aCGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGgga                   >  4:86407/1‑148 (MQ=40)
                                                                                                                                       tAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAg     <  7:31682/148‑1 (MQ=255)
                                                                                                                                          tCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGGTCCCGGGAGGACCGCGCTGCCGGcc  >  6:160814/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                              
CAGCGTCGCCATCGCAAACGGACGGTTCGCTGAGTCAAAACAGACGAGTTCGCATCTTGCTTCTTGCTTCTTGCTTCTTGCTTCTTGGATGTGGGCATTCACATCGAGTAGGTATTCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCC  >  NC_002947/4022490‑4022775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑