Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,348,7050AG40.4% 38.4 / 55.6 52intergenic (‑70/+404)PP_3820/galUgroup II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (17/14);  new base G (9/12);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACC  >  NC_002947/4348591‑4348848
                                                                                                                  |                                                                                                                                               
cTGCTTTACGGTTAACAACGAGACGGCGAAGACTACTGGCGGCATAACCTGTTCGGCCCTTGGTGTCGAGGTTAACAGCCACTTACTACGGCTTCGGCTGACTTCTTCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGt                                                                                                                 <  4:125055/147‑1 (MQ=11)
    aTTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCaa                                                                                                            >  3:5290/1‑148 (MQ=17)
    aTTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCaa                                                                                                            >  3:124280/1‑148 (MQ=17)
    aTTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCaa                                                                                                            <  5:127282/148‑1 (MQ=32)
         ggTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTTCGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGt                                                                                                        >  3:19119/1‑147 (MQ=21)
                aaCGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGAtct                                                                                                >  4:29809/1‑148 (MQ=21)
                aaCGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGAtct                                                                                                >  8:75830/1‑148 (MQ=34)
                aaCGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGAtct                                                                                                >  1:41255/1‑148 (MQ=34)
                    agaCGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCa                                                                                            <  7:54661/148‑1 (MQ=21)
                        ggCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGCCGCGCGGTAGCGCAGTGGCAAGCGTACAGATCTCCCAggg                                                                                         >  8:128359/1‑147 (MQ=255)
                          cGACGCCTACTGGCGGCATAACCTGTTCGGCCATTGGTGGAGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTaa                                                                                      >  2:128277/1‑148 (MQ=21)
                               ccTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTcgc                                                                                 <  1:84109/148‑1 (MQ=34)
                               ccTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTcgc                                                                                 <  1:146855/148‑1 (MQ=34)
                                 tACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCTGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTcgcgc                                                                               <  6:43002/148‑1 (MQ=21)
                                 tACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTcgcgc                                                                               <  4:10665/148‑1 (MQ=21)
                                      gcggcATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCCCTTCCTACGGCTTCGGCGGACTTCTGCGCGCTCATCCCGTCGCCTTTCGCCGCGCGGTAGCACAGTGGCAAGCGTCGAGATCTCGCAGGGGAATTCGCGCGAccg                                                                          >  1:83715/1‑147 (MQ=11)
                                      gcggcATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCt                                                                          >  5:159916/1‑148 (MQ=34)
                                          cATAACCTGTTCGGCCCTTGGTGGCGCAGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCt                                                                      <  7:57974/148‑1 (MQ=21)
                                             aaCCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCTCGACCTTCCTGc                                                                    >  6:103677/1‑147 (MQ=21)
                                               ccTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTTCGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTa                                                                 >  4:69559/1‑148 (MQ=21)
                                               ccTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGAcc                                                                           >  1:28155/1‑138 (MQ=21)
                                                cTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCt                                                                          <  2:28155/138‑1 (MQ=21)
                                                   ttCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTcgcgc                                                                               >  1:61389/1‑130 (MQ=35)
                                                    tCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTcgcgcg                                                                              <  2:61389/130‑1 (MQ=35)
                                                       gCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTc                                                         <  1:124577/148‑1 (MQ=34)
                                                       gCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGACAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTc                                                         <  4:105289/148‑1 (MQ=21)
                                                               tggCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTAc                                                 <  6:109639/148‑1 (MQ=21)
                                                               tggCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTAc                                                 <  1:116782/148‑1 (MQ=34)
                                                                  cgcgGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGt                                               <  6:73441/147‑1 (MQ=34)
                                                                            cGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTcc                                    >  3:14543/1‑148 (MQ=21)
                                                                            cGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTcc                                    >  6:68272/1‑148 (MQ=34)
                                                                            cGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCACGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTcc                                    <  1:164089/148‑1 (MQ=21)
                                                                              ccACTTACTACGGCTTAGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCAACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGt                                  >  3:123241/1‑148 (MQ=21)
                                                                                  ttACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCaa                              <  2:123453/148‑1 (MQ=34)
                                                                                         ggCTTCGGCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTgg                       <  8:129026/148‑1 (MQ=21)
                                                                                            ttCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCt                    >  5:37660/1‑148 (MQ=34)
                                                                                                gCTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTg                 <  2:69403/147‑1 (MQ=21)
                                                                                                 cTGACTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGGCACAGCGTTCCGTGCAAGTATTGGGCTTTgg                >  1:136145/1‑147 (MQ=21)
                                                                                                 cTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGc               <  5:35597/148‑1 (MQ=34)
                                                                                                  tGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCg              >  1:108972/1‑148 (MQ=34)
                                                                                                  tGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCg              >  3:35926/1‑148 (MQ=34)
                                                                                                     cTTCTGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGAt            <  4:19119/147‑1 (MQ=21)
                                                                                                      ttCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGAttt          >  5:85011/1‑148 (MQ=34)
                                                                                                         tGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTtgt        <  8:45435/147‑1 (MQ=21)
                                                                                                         tGCACGCTCGTCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTtgt        <  4:124280/147‑1 (MQ=21)
                                                                                                         tGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTtgtg       >  7:43316/1‑148 (MQ=34)
                                                                                                         tGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTtgt        <  7:71084/147‑1 (MQ=34)
                                                                                                         tGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTtgt        <  5:68272/147‑1 (MQ=34)
                                                                                                             cGCTCGTCCCGTCGCCTTTCGACGCGCGGGAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCAc   <  5:94802/148‑1 (MQ=21)
                                                                                                              gCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCAcc  >  6:132846/1‑148 (MQ=34)
                                                                                                              gCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCAcc  >  3:27182/1‑148 (MQ=34)
                                                                                                              gCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCAcc  >  3:75696/1‑148 (MQ=34)
                                                                                                                  |                                                                                                                                               
CTGCATTACGGTTACCAACGAGACGGCGACGCCTACTGGCGGCATAACCTGTTCGGCCCTTGGTGGCGCGGTTAACCGCCACTTACTACGGCTTCGGCTGACTTCTGCACGCTCATCCCGTCGCCTTTCGACGCGCGGTAGCACAGTGGCAAGCGTACAGATCTCCCAGGGTAATTCGCGCGACCTTCCTGCTTATGCCTGTCGGATCTACGTCACAGCGTTCCGTGCAAGTATTGGGCTTTGGCGATTTGTGCCACC  >  NC_002947/4348591‑4348848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑